The efficacy of X-ray doses on murine norovirus-1 (MNV-1) in pure culture, half-shell oyster, salmon sushi, and tuna salad

Wu, Yang; Chang, Sam K. C.; Nannapaneni, Ramakrishna; Coker, R.; Haque, Zee; Mahmoud, Barakat S.M.

doi:10.1016/j.foodcont.2015.12.018

Cited by 11 publications

(6 citation statements)

References 21 publications

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“…(Cox et al, 2009;Gonzalez-Hernandez et al, 2012). The MNV-1 stocks were prepared and stored as previously described (Wu, et al, 2016).…”

Section: Murine Norovirus (Mnv-1)mentioning

confidence: 99%

“…Plaque assays were performed as described by Wu et al, (2016). A series of 10-fold dilutions of sample were inoculated onto the 6-well plates after the formation of a confluent monolayer of BV-2, aspiration of the medium and two cell washes with DMEM-0.…”

Section: Mnv-1 Enumeration (Plaque Assay)mentioning

confidence: 99%

“…Treatment with 3.0 kGy X-ray treatment did not affect the survivability of live oysters. Wu et al, (2016) stated that X-ray is a potential antiviral agent against HuNoVs surrogate murine norovirus (MNV-1) in food products including salmon sushi and tuna salad. We have to rely on proper surrogates to study the survival of human of HuNoV because HuNoV is not cultivable (Ye et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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The effects of X-ray treatments on bioaccumulated murine norovirus-1 (MNV-1) and survivability, inherent microbiota, color, and firmness of Atlantic oysters (Crassostrea virginica) during storage at 5 °C for 20 days

Chang

Nannapaneni

et al. 2017

Food Control

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In this study, we investigated the inactivation of human norovirus (HuNoV) surrogate Murine norovirus (MNV-1) by X-ray in whole-shell Atlantic oysters (Crassostrea virginica). We also investigated the effects of X-ray treatments on the survivability, inherent microbiota, color, and firmness of treated whole-shell Atlantic oysters during storage at 5 o C for 20 days. Plaque assays revealed that X-ray significantly reduced the population of internalized MNV-1 in live oysters from 4.3 ± 0.4 log PFU g-1 to 3.6 ± 0.5, 3.2 ± 0.2, 2.8 ± 0.2, and 2.5 ± 0.1 log PFU g-1 , by 1.0, 2.0, 3.0, and 4.0 kGy X-ray, respectively. The population of MNV-1 was reduced to less than 2.0 log PFU g-1 at 5.0 kGy X-ray. The survivability of live oysters was not significantly affected by treatment with 1.0 and 5.0 kGy X-ray, in comparison with the control, for up to 15 and 10 days, respectively, during storage at 5°C. Treatment of whole-shell oysters by 5.0 kGy X-ray significantly reduced the inherent microbiota and kept them lower than the control until day 20. No significant effect of X-ray treatment on oyster color or firmness was detected.

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“…(Cox et al, 2009;Gonzalez-Hernandez et al, 2012). The MNV-1 stocks were prepared and stored as previously described (Wu, et al, 2016).…”

Section: Murine Norovirus (Mnv-1)mentioning

confidence: 99%

Section: Mnv-1 Enumeration (Plaque Assay)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effects of X-ray treatments on bioaccumulated murine norovirus-1 (MNV-1) and survivability, inherent microbiota, color, and firmness of Atlantic oysters (Crassostrea virginica) during storage at 5 °C for 20 days

Chang

Nannapaneni

et al. 2017

Food Control

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“…In what may be considered the most prescient of irradiation applications was the use in NASA’s space program. The U.S. FDA approves the use of irradiation as a PHP for oysters and has been validated by a number of researchers [ 19 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”

Section: Irradiationmentioning

confidence: 99%

Food Safety Impacts from Post-Harvest Processing Procedures of Molluscan Shellfish

Baker

2016

Foods

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Post-harvest Processing (PHP) methods are viable food processing methods employed to reduce human pathogens in molluscan shellfish that would normally be consumed raw, such as raw oysters on the half-shell. Efficacy of human pathogen reduction associated with PHP varies with respect to time, temperature, salinity, pressure, and process exposure. Regulatory requirements and PHP molluscan shellfish quality implications are major considerations for PHP usage. Food safety impacts associated with PHP of molluscan shellfish vary in their efficacy and may have synergistic outcomes when combined. Further research for many PHP methods are necessary and emerging PHP methods that result in minimal quality loss and effective human pathogen reduction should be explored.

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“…Therefore, it is necessary to establish an efficient NoV enrichment method to minimize the influence of the inhibitors. Traditional enrichment methods include direct treatment, proteinase K digestion and proteinase K digestion combined with polyethylene glycol precipitation ( Lee and Lee, 1981 ; Wu et al, 2016 ). However, these methods are non-specific and cannot fully remove the interference of complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Efficiency Immunomagnetic Enrichment Method for Detection of Human Norovirus via PAMAM Dendrimer/SA-Biotin Mediated Cascade-Amplification

et al. 2021

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Human norovirus is a common cause of acute gastroenteritis worldwide, and oysters have been found to be the main carriers for its spread. The lack of efficient pre-treatment methods has been a major bottleneck limiting the detection of viruses in oysters. In this study, we established a novel immunomagnetic enrichment method using polyamidoamine (PAMAM) dendrimer/SA-biotin-mediated cascade amplification for reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) detection. We compared the capture efficiency of traditional immunomagnetic enrichment, biotin-amplified immunomagnetic enrichment, and PAMAM dendrimer/SA-biotin-mediated cascade-amplification immunomagnetic enrichment. The optimal capture efficiency of the novel method was 44.26 ± 1.45%, which increased by 183.17% (P < 0.01) and 18.09% (P < 0.05) compared with the first two methods, respectively. Three methods were all applied in detecting norovirus in 44 retail oysters, the detection rate of the PAMAM dendrimer/SA-biotin-mediated method was 25.0%, which was higher than those of traditional IME (15.90%) and SA-biotin-amplified IME (18.80%) by 9.1 and 6.2%, respectively. In conclusion, the novel method can be applied for the rapid detection of norovirus in oysters, which can help reduce the cost and time of detection and improve detection rates.

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The efficacy of X-ray doses on murine norovirus-1 (MNV-1) in pure culture, half-shell oyster, salmon sushi, and tuna salad

Cited by 11 publications

References 21 publications

The effects of X-ray treatments on bioaccumulated murine norovirus-1 (MNV-1) and survivability, inherent microbiota, color, and firmness of Atlantic oysters (Crassostrea virginica) during storage at 5 °C for 20 days

The effects of X-ray treatments on bioaccumulated murine norovirus-1 (MNV-1) and survivability, inherent microbiota, color, and firmness of Atlantic oysters (Crassostrea virginica) during storage at 5 °C for 20 days

Food Safety Impacts from Post-Harvest Processing Procedures of Molluscan Shellfish

Development of a High-Efficiency Immunomagnetic Enrichment Method for Detection of Human Norovirus via PAMAM Dendrimer/SA-Biotin Mediated Cascade-Amplification

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