The Efficacy of Viral Capsid Inhibitors in Human Enterovirus Infection and Associated Diseases

Li, Chin; Wang, Hongtao; Shih, Shin‐Ru; Chen, Tzu‐Chun; Li, Meiling

doi:10.2174/092986707780363032

Cited by 33 publications

(22 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Li et al 2007; Vaddiraju et al 2010) Miniaturization, however, stands as a major impediment for amperometric techniques, since reduction in the area of the working electrode generally leads to weaker signal intensity that rapidly approaches noise level. To mitigate this challenge, miniaturized amperometric biosensors are constantly advancing the following four research frontiers:

Enhancement of surface area of the working electrodes by increasing nanoporosity using metal etching and decorating techniques,(Erlebacher et al 2001),(You et al 2003) conducting polymers (Singh et al 2009), carbon nanotubes,(Yu et al 2006) and various nanostructured semiconductors.

…”

Section: Introductionmentioning

confidence: 99%

Edge-plane microwire electrodes for highly sensitive H2O2 and glucose detection

Qiang

Vaddiraju

Patel

et al. 2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

The promise of implantable electrochemical sensors is often undermined by the critical requirement of device miniaturization that inadvertently degrades sensor performance in terms of sensitivity and selectivity. Herein, we report a novel miniaturized and flexible amperometric sensor grown at the ‘edge plane’ of a 25-µm gold wire. Such geometry affords extreme miniaturization along with ease of fabrication, minimal iR drop and 3-D diffusion for effective mass transfer. This together with electrochemical rebuilding of the Au working electrode and subsequent Pt nanoparticles deposition resulted in the highest H2O2 sensitivity (33 mA·mM−1·cm−2), reported thus far. Concurrent electrodeposition of o-phenylenediamine with glucose oxidase afforded glucose detection at these edge-plane microsensors with a six fold improvement in sensitivity (1.2 mA·mM−1·cm−2) over previous reports. In addition, these sensors exhibit low operation potential (0.3 V), high selectivity (more than 95%) against in vivo interferences, and an apparent Michealis-Menten constant (Kmitalicapp) of 17 and 75 mM of glucose in the absence and presence of an outer polyurethane coating, respectively. These features render the edge-plane sensor architecture as a powerful platform for next-generation implantable biosensors.

show abstract

…”

Section: Introductionmentioning

confidence: 99%

Edge-plane microwire electrodes for highly sensitive H2O2 and glucose detection

Qiang

Vaddiraju

Patel

et al. 2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…VP1, VP2 and VP3 each contain 270 amino acid residues and are located in the exterior of the viral particle. VP1 plays critical roles in adsorption and the uncoating process of the virus [29]. Previous studies showed that the VP1 capsid protein can bind three human proteins including ornithine decarboxylase (ODC1), gene trap ankyrin repeat (GTAR) and KIAA0697.…”

Section: Discussionmentioning

confidence: 99%

GITRL as a genetic adjuvant enhances enterovirus 71 VP1 DNA vaccine immunogenicity

et al. 2015

View full text Add to dashboard Cite

VP1 protein is the immunodominant capsid protein of enterovirus 71 (EV71) which is responsible for large outbreaks of hand, foot and mouth disease. It has been reported that glucocorticoid-induced tumor necrosis factor receptor-related protein (GITR) and its ligand (GITRL) are involved in modulating both innate and adaptive immune responses. In this study, a DNA vaccine vector encoding EV71 VP1 gene and mGITRL gene (pIRES/VP1/mGITRL) was constructed. And female Balb/c mice were immunized intramuscularly with the DNA vaccine. Compared with the groups immunized with pIRES, pIRES/VP1, pIRES/mGITRL and PBS, the inoculation of pIRES/VP1/mGITRL induced a higher levels of EV71 VP1-specific antibody and specific antibody-forming cells. However, significantly higher levels of CD4(+)Th1, Th2 and CD8(+)IFN-γ(+)T cells were found in the pIRES/VP1/mGITRL group compared with control groups. Our results demonstrate that a novel DNA vaccine, expressing VP1 and mGITRL, could effectively elicit both humoral and cell-mediated immune responses against EV71 VP1 in mice. Thus, the mGITRL may be used as molecular adjuvant for EV71 DNA vaccine.

show abstract

“…45 If enteroviruses are found to be a major contributor to its development, then renewed efforts at developing an effective polyvalent enterovirus vaccine, 46,47 and antiviral agents such as pleconaril [48][49][50][51] are certain to follow. This will enable the protection against and/or treatment of enterovirus infections during childhood, to prevent, or at least reduce, both the short-term and long-term complications of these viruses.…”

Section: 8smentioning

confidence: 99%