The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis

Kim, Young Ock; Hong, Seung Jae; Yim, Sung-Vin

doi:10.4196/kjpp.2010.14.4.199

Cited by 24 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The expression levels of IL-6 were also inhibited. More recently, Kim et al [22] published a study on the efficacy of shikonin in protecting the cartilage in a murine model of rheumatoid arthritis, also induced by collagen. In this case, the authors used a dose of 2 mg/kg of shikonin administered orally daily for 35 days.…”

Section: Shikonin In Autoimmune-mediated Inflammatory Diseasesmentioning

confidence: 99%

Pharmacological Properties of Shikonin – A Review of Literature since 2002

et al. 2013

View full text Add to dashboard Cite

!The naphthoquinone shikonin is the main active principle of Zicao, a traditional Chinese herbal medicine made from the dried root of Lithospermum erythrorhizon. Studies carried out over the past 30 years have provided a scientific basis for the use of Zicao which has been long employed in folk medicine to treat a variety of inflammatory and infectious diseases. In particular, shikonin has been shown to possess many diverse properties, including antioxidant, anti-inflammatory, antithrombotic, antimicrobial, and wound healing effects. The fact that shikonin shows so many beneficial properties has increased the interest in this molecule dramatically, especially in the past few years. The aim of this review is to provide an update of the new data published on shikonin, whose wide spectrum of pharmacological effects as well as pharmacokinetic properties and toxicity make it a highly interesting target molecule.

show abstract

Section: Shikonin In Autoimmune-mediated Inflammatory Diseasesmentioning

confidence: 99%

Pharmacological Properties of Shikonin – A Review of Literature since 2002

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In this study, we examined the effects of LES, as a novel osteogenic material, on osteoblast differentiation. In previous studies, shikonin, from the root of Lithospermum erythrorhizon, has been shown to protect cartilage in rheumatoid arthritis, but also to induce osteoclastogenesis in vitro (38,39). However, hydrophobic shikonin and its derivatives are not extracted in the water-soluble EtOH extract, LES.…”

Section: Discussionmentioning

confidence: 99%

“…Shikonin plays a dual role in the regulation of the early and late stages of collagen type II arthritis (37). Shikonin exerts protective effects on cartilage in rheumatoid arthritis (38), but also induces osteoclast differentiation in vitro (39). However, the function of LR in osteoblast differentiation remains unknown.…”

Section: Ethanol Extract Of Lithospermum Erythrorhizonmentioning

confidence: 99%

Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix

Choi

Kim

Choi

et al. 2016

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Bone remodeling and homeostasis are largely the result of the coordinated action of osteoblasts and osteoclasts. Osteoblasts are responsible for bone formation. The differentiation of osteoblasts is regulated by the transcription factors, Runx2 and Osterix. Natural products of plant origin are still a major part of traditional medicinal systems in Korea. The root of Lithospermum erythrorhizon Sieb. et Zucc. (LR), the purple gromwell, is an herbal medicine used for inflammatory and infectious diseases. LR is an anti-inflammatory and exerts anticancer effects by inducing the apoptosis of cancer cells. However, the precise molecular signaling mechanisms of osteoblastogenesis as regards LR and osteoblast transcription are not yet known. In this study, we investigated the effects of ethanol (EtOH) extract of LR (LES) on the osteoblast differentiation of C2C12 myoblasts induced by bone morphogenetic protein 4 (BMP4) and the potential involvement of Runx2 and Osterix in these effects. We found that the LES exhibited an ability to induce osteoblast differentiation. LES increased the expression of the osteoblast marker, alkaline phosphatase (ALP), as well as its activity, as shown by ALP staining and ALP activity assay. LES also increased mineralization, as shown by Alizarin Red S staining. Treatment with LES increased the protein levels (as shown by immunoblotting), as well as the transcriptional activity of Runx2 and Osterix and enhanced osteogenic activity. These results suggest that LES modulates osteoblast differentiation at least in part through Runx2 and Osterix.

show abstract

“…CIA is an autoimmune-mediated polyarthritis that shares important similarities with RA in histology and immunology [16,17]. The immune reaction of CIA rats is regulated by complex mechanisms, which are incompletely understood, but regulatory T cells are able to suppress the activation of CD4+ T cells [18].…”

Section: Discussionmentioning

confidence: 99%

Dynamic Frequency of Blood CD4+CD25+ Regulatory T Cells in Rats with Collagen-induced Arthritis

Wang

Jin

et al. 2015

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs)have been shown to play a regulatory or suppressive role in the immune response and are possibly relevant to the pathogenesis of autoimmune diseases. In the present study, we attempted to investigate the frequency of CD4+CD25+ Tregs in peripheral blood (PB) of collagen-induced arthritis (CIA) rats during the development of arthritis, to determine whether their frequency is involved in the immunoregulation of this disease. The results showed that normal rats had similar frequencies of CD4+CD25+ Tregs in PB during the experiment time, expressed as a percentage of CD4+CD25+Foxp3+ T cells among the CD4+ T lymphocyte population. In contrast, the frequency of CD4+CD25+Foxp3+ T cells in CIA rats was found to change during the development of arthritis. In CIA rats, there is a significant negative correlation between the frequency of CD4+CD25+Foxp3+ T cells and paw swelling (r=− 0.786, p＜ 0.01). The relationship between the frequency of CD4+CD25+Foxp3+ T and immune activation was not found in normal rats. During the time course, the frequency of CD4+CD25+Foxp3+ T was lower in CIA rats than in normal ones. The data suggest that the frequency of PB CD4+CD25+ Tregs may be a promising marker for arthritis activity.

show abstract

The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis

Cited by 24 publications

References 25 publications

Pharmacological Properties of Shikonin – A Review of Literature since 2002

Pharmacological Properties of Shikonin – A Review of Literature since 2002

Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix

Dynamic Frequency of Blood CD4+CD25+ Regulatory T Cells in Rats with Collagen-induced Arthritis

Contact Info

Product

Resources

About