The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-Producing Escherichia coli from Pig and Turkey Farms

Skaradzińska, Aneta; Śliwka, Paulina; Kuźmińska-Bajor, Marta; Skaradziński, Grzegorz; Rząsa, Anna; Friese, Anika; Roschanski, Nicole; Murugaiyan, Jayaseelan; Roesler, Uwe

doi:10.3389/fmicb.2017.00530

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…The host ranges of the phages were determined on a diverse E. coli strain collection including clinical ESBL-producing E. coli , ESBL-producing E. coli isolated from poultry carcasses and APEC strains. As shown by others, phages show similar lytic activity on ESBL-producing bacteria and bacteria without ESBL production [ 60 , 61 , 62 ]. The morphologically T4-like phages TB49 and G28 exhibited broad spectra of lytic activity, whereas the other phages of the cocktail had relatively narrow spectra that targeted E28 very specifically.…”

Section: Discussionmentioning

confidence: 62%

In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli

Korf

Kittler

Bierbrodt³

et al. 2020

Viruses

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Worldwide, poultry industry suffers from infections caused by avian pathogenic Escherichia coli. Therapeutic failure due to resistant bacteria is of increasing concern and poses a threat to human and animal health. This causes a high demand to find alternatives to fight bacterial infections in animal farming. Bacteriophages are being especially considered for the control of multi-drug resistant bacteria due to their high specificity and lack of serious side effects. Therefore, the study aimed on characterizing phages and composing a phage cocktail suitable for the prevention of infections with E. coli. Six phages were isolated or selected from our collections and characterized individually and in combination with regard to host range, stability, reproduction, and efficacy in vitro. The cocktail consisting of six phages was able to inhibit formation of biofilms by some E. coli strains but not by all. Phage-resistant variants arose when bacterial cells were challenged with a single phage but not when challenged by a combination of four or six phages. Resistant variants arising showed changes in carbon metabolism and/or motility. Genomic comparison of wild type and phage-resistant mutant E28.G28R3 revealed a deletion of several genes putatively involved in phage adsorption and infection.

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Section: Discussionmentioning

confidence: 62%

In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli

Korf

Kittler

Bierbrodt³

et al. 2020

Viruses

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“…[ 18 ] reported that phages isolated from poultry and pig farms could be used in the biocontrol of specific poultry and pig pathogens, while another study demonstrated that 17 specific E. coli phages isolated from pig farms were active against both related and unrelated ESBL/AmpC E. coli isolates. Interestingly, 14/17 phages were more effective against ESBL/AmpC E. coli isolated from turkey farms than that from pig farms [ 19 ]. This indicated that not the site of isolation but rather phage–host interaction determines the infectibility of phages.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study

et al. 2022

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Background and Aim: Swine enteric colibacillosis caused by Escherichia coli is a major problem in the swine industry, causing diarrhea among swine and resulting in substantial financial losses. However, efforts to counter this disease are impeded by the increase in antimicrobial resistance (AMR) worldwide, so intensive research is being conducted to identify alternative treatments. This study isolated, characterized, and evaluated the efficacy of bacteriophages to control pathogens causative of swine enteric colibacillosis. Materials and Methods: Five sewage samples were collected from different areas of a swine farm in Suphanburi province, Thailand and the bacteriophages were enriched and isolated, followed by purification by the agar overlay method using E. coli RENR as the host strain. The selected phages were characterized by evaluating their morphology, while their specificity was verified by the host range test. The efficiency of plating and multiplicity of infection (MOI) were also determined. Results: Four selected phages, namely, vB_Eco-RPNE4i3, vB_Eco-RPNE6i4, vB_Eco-RPNE7i1, and vB_Eco-RPNE8i3, demonstrated different patterns of host range and phage efficiency. They significantly decreased E. coli concentration at the tested MOIs (0.01–100) from 1 h onward. However, bacterial regrowth was observed in all phage treatments. Conclusion: This study shows the potential of using phages as an alternative treatment for swine enteric colibacillosis. The obtained results demonstrated that the selected phages had a therapeutic effect against pathogens causative of swine enteric colibacillosis. Therefore, phages could be applied as an alternative treatment to control specific bacterial strains and reduce AMR arising from the overuse of antibiotics.

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“…Moreover, in our previous research, we hypothesized that the role of phages may not be limited to directly target the pathogens, but bacterial viruses may also be applied against the spread of bacteria with antibiotic-resistance genes in animal production [ 16 ]. In that study, we isolated 17 E. coli -specific bacteriophages from samples taken on Polish pig farms.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment

Śliwka

Weber‐Dąbrowska

Żaczek

et al. 2023

IJMS

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The emerging global crisis of antibiotic resistance demands new alternative antibacterial solutions. Although bacteriophages have been used to combat bacterial infections for over a century, a dramatic boost in phage studies has recently been observed. In the development of modern phage applications, a scientific rationale is strongly required and newly isolated phages need to be examined in detail. In this study, we present the full characterization of bacteriophages BF9, BF15, and BF17, with lytic activity against extended-spectrum β-lactamases (ESBLs)- and AmpC β-lactamases (AmpC)-producing Escherichia coli, the prevalence of which has increased significantly in livestock in recent decades, representing a great hazard to food safety and a public health risk. Comparative genomic and phylogenetic analysis indicated that BF9, BF15, and BF17 represent the genera Dhillonvirus, Tequatrovirus, and Asteriusvirus, respectively. All three phages significantly reduced in vitro growth of their bacterial host and retained the ability to lyse bacteria after preincubation at wide ranges of temperature (−20–40 °C) and pH (5–9). The results described herein indicate the lytic nature of BF9, BF15, and BF17, which, along with the absence of genes encoding toxins and bacterial virulence factors, represents an undoubted asset in terms of future phage application.

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The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-Producing Escherichia coli from Pig and Turkey Farms

Cited by 13 publications

References 33 publications

In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli

In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli

Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study

Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment

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