“…However, high levels of chlorantraniliprole resistance in DBM are reported in China, Thailand, the Philippines and Brazil . Despite this developing resistance, chlorantraniliprole and other diamide insecticides continue to play a very important role in the management of P. xylostella and other pest insects worldwide . Therefore, the mechanisms of resistance of DBM to chlorantraniliprole must be clearly understood.…”
“…However, high levels of chlorantraniliprole resistance in DBM are reported in China, Thailand, the Philippines and Brazil . Despite this developing resistance, chlorantraniliprole and other diamide insecticides continue to play a very important role in the management of P. xylostella and other pest insects worldwide . Therefore, the mechanisms of resistance of DBM to chlorantraniliprole must be clearly understood.…”
“…Recent outbreaks of M. separata have been observed in several regions of China, especially Jilin, Hebei, Heilongjiang, Liaoning, and Shanxi, and this organism poses a severe threat to corn production ( Yun et al, 2017 ). In an attempt to restrict crop damage by M. separata , high doses of synthetic insecticides are often applied ( Liu Y. et al, 2016 ), but this causes serious environmental problems and cropland deterioration, stimulates insect resistance, and has a negative impact on non-target organisms ( Younas et al, 2016 ). Population outbreaks of M. separata present a great challenge in terms of protecting crops worldwide ( Liu et al, 2017 ).…”
Chemosensory proteins (CSPs) play important roles in chemosensation in insects, but their exact physiological functions remain elusive. In order to investigate the functions of CSPs in the oriental armyworm Mythimna separata, in the present study we explored expression patterns and binding characteristics of the CSP, MsepCSP8. The distinctive functions of MsepCSP8 were also validated by RNAi. The results showed that MsepCSP8 shares high sequence similarity with CSPs of other insect family members, including the characteristic four-cysteine signature motif. MsepCSP8 mRNA was specifically expressed in antennae of females at levels well above those in other tissues. Competitive binding assays confirmed that 20 out of 56 ligands bound more strongly to MsepCSP8 at pH 7.4 than at pH 5.0. Protein structure modeling and molecular docking analyses identified amino acid residues involved in binding volatile compounds, and behavioral response experiments showed that M. separata elicited significant responses to five volatiles from compounds displaying high binding affinity to MsepCSP8. MsepCSP8 transcript abundance was decreased by dsMsepCSP8 injection, which affected the behavioral responses of M. separata to representative semiochemicals. Our findings demonstrate that MsepCSP8 likely contributes to mediating responses of M. separata adults to plant volatiles.
“…Despite this, chlorantraniliprole and other diamide insecticides, such as flubendiamide and cyantraniliprole, remain very important for the management of P. xylostella and other pest insects worldwide . To make good use of these novel insecticides and increase their service life, an in‐depth understanding of resistance mechanisms is necessary.…”
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