The Efficacy of Diaspirin Crosslinked Hemoglobin Solution Resuscitation in a Model of Uncontrolled Hemorrhage

Schultz, Scot C.; Powell, Craig C.; Burris, David; Nguyen, Huy B.; Jaffin, Jonathan H.; Malcolm, Diana

doi:10.1097/00005373-199409000-00013

Cited by 46 publications

(19 citation statements)

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“…Modified, cell‐free hemoglobin oxygen‐carrying solutions may eventually overcome the limitations of homologous blood transfusions and are currently undergoing clinical trials (1). Maintenance of cardiovascular function and oxygen transport has been demonstrated after partial and complete exchange transfusion with cell‐free hemoglobin solutions (2–4). Hemoglobin solutions have been considered a superior resuscitative fluid because of their pharmacological actions, which cause increases in arterial pressure and blood flow, even in small doses (1–5).…”

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confidence: 99%

Effects of Hemoglobin‐Based Blood Substitutes on Vasoactivity of Rat Aortic Rings

et al. 2001

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Our objective is to characterize the vasoactive properties of a 10% alphaalpha diaspirin cross-linked human hemoglobin (alphaalphaHb) and to test the hypothesis that sodium nitroprusside (SNP)-induced relaxation is inhibited in the presence of alphaalphaHb. Experiments were performed on aortic rings from 18 Wistar rats; the rings were suspended in aerated Krebs solution. Changes in isometric tension were measured to increasing concentrations of alphaalphaHb (1.8 x 10(-9) to 10(-4) M) on phenylephrine (PE)-induced contraction (3 x 10(-7) M), on acetylcholine (ACh)-induced relaxation (10(-8) to 10(-6) M), on SNP-induced relaxation (10(-9) and 10(-8) M), and on PE-induced contraction with an endothelin-1 (ET1) receptor antagonist, BQ123 (10(-5) M). Control rings received no alphaalphaHb. A concentration-dependent increase of the PE-precontraction (1.3%, 6.8%, 17.4%, and 34%, respectively) as well as the inhibition and reversal of ACh-induced relaxation was observed after alphaalphaHb. The presence of alphaalphaHb decreased the SNP-induced relaxation in the presence or absence of endothelium. The relaxation induced by SNP was reduced with time in the presence, but not in the absence, of alphaalphaHb. In conclusion, although pharmacological modulation of the vasoconstriction is possible with nitric oxide donors, our findings suggest that in the clinical setting, large sustained donor doses may be required.

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Effects of Hemoglobin‐Based Blood Substitutes on Vasoactivity of Rat Aortic Rings

et al. 2001

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“…Several other investigators used various animal models of hemorrhage to document similar hemodynamic responses following treatment with DCLHb [113][114][115][116]. Other endpoints of effective resuscita-tion such as base de cit correction [117], intestinal mucosal oxygen tension [118], and central venous oxygen saturation [119] responded favorably with DCLHb compared with traditional resuscitative uids.…”

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confidence: 99%

Hemoglobin Solutions as Blood Substitutes

Friedman

DeVenuto²,

Kerwin³

et al. 2000

Journal of Investigative Surgery

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“…Measuring hemodynamic parameters that correlate with cardiac work can assess the physical stress to the cardiovascular system. Diaspirin crosslinked hemoglobin (DCLHb, Baxter Healthcare, Irvine, CA, USA) is a stable, covalently crosslinked hemoglobin derivative that has been extensively tested and found to be effective as a resuscitative fluid following hemorrhage [4][5][6]. The Ps0 of oxygen binding by DCLHb is approximately 32 +_ 3mmHg, resembling the Ps0 of swine blood in vivo [7].…”

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confidence: 99%

Effects of diaspirin crosslinked hemoglobin infusion in treadmill-exercised swine

1999

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The hemodynamic and metabolic effects of diaspirin crosslinked hemoglobin (DCLHb) were investigated using graded treadmill exercise in swine (n = 5/group). Swine received DCLHb (10% solution, 5 ml/kg) or oncotically-matched human serum albumin (HSA, 5ml/kg). Baseline metabolic and hemodynamic data were similar. In both groups exercise increased hemodynamic parameters. Exercise increased heart rate (HR) from 139 +/- 12 to 293 +/- 28 bpm with DCLHb and from 136 +/- 13 to 314 +/- 13 bpm with HSA. Exercise increased cardiac output (CO) from 5.7 +/- 0.75 to 15.6 +/- 2.01/min in the DCLHb group and from 5.3 +/- 0.48 to 15.7 +/- 0.881/min in the HSA group. However, CO returned to baseline faster with DCLHb upon stopping exercise. The DCLHb-treated group demonstrated a significantly higher oxygen extraction during exercise (12.04 +/- 0.38 vs 9.48 +/- 0.99 ml O2/100 ml blood) and a lower oxygen delivery throughout recovery (74.6 +/- 6.6 vs 102.2 +/- 7.21 O2/min), indicating enhanced oxygen delivery during exercise in the treatment group. DCLHb infusion did not impair metabolic or hemodynamic functions. These data indicate that DCLHb may increase oxygen delivery to working tissue more efficiently than HSA during treadmill exercise in swine.

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The Efficacy of Diaspirin Crosslinked Hemoglobin Solution Resuscitation in a Model of Uncontrolled Hemorrhage

Cited by 46 publications

References 0 publications

Effects of Hemoglobin‐Based Blood Substitutes on Vasoactivity of Rat Aortic Rings

Effects of Hemoglobin‐Based Blood Substitutes on Vasoactivity of Rat Aortic Rings

Hemoglobin Solutions as Blood Substitutes

Effects of diaspirin crosslinked hemoglobin infusion in treadmill-exercised swine

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