The Efficacy of Colistin Combined with Amikacin or Levofloxacin against Pseudomonas aeruginosa Biofilm Infection

Wang, Yuhang; Li, Chunsun; Wang, Jin; Bai, Nan; Zhang, Huan; Chi, Yulong; Cai, Yun

doi:10.1128/spectrum.01468-22

Cited by 5 publications

(2 citation statements)

References 28 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, Gómez-Juyent, J et al reported that these combinations were the most appropriate treatments for biofilm-related infections [ 54 ] and Armengol, E et al showed how COL enhances rifampicin’s antimicrobial activity in COL-resistant biofilms [ 55 ]. Wang Y et al validated the effectiveness of the AMK–COL combination not only in vitro but also in an animal biofilm infection model [ 56 ]. Other antibiotic combinations have also been reported to be more effective than monotherapy [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotics against Pseudomonas aeruginosa on Human Skin Cell Lines: Determination of the Highest Non-Cytotoxic Concentrations with Antibiofilm Capacity for Wound Healing Strategies

Quiñones-Vico,

Fernández-González,

Ubago-Rodríguez

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

Pseudomonas aeruginosa is one of the most common microorganisms causing infections of severe skin wounds. Antibiotic or antiseptic treatments are crucial to prevent and curb these infections. Antiseptics have been reported to be cytotoxic to skin cells and few studies evaluate the impact of commonly used antibiotics. This study evaluates how clinical antibiotics affect skin cells’ viability, proliferation, migration, and cytokine secretion and defines the highest non-cytotoxic concentrations that maintain antibacterial activity. Cell proliferation, viability, and migration were evaluated on cell monolayers. Cytokines related to the wound healing process were determined. The minimum inhibitory concentrations and the impact on bacterial biofilm were assessed. Results showed that 0.02 mg/mL ciprofloxacin and 1 mg/mL meropenem are the highest non-cytotoxic concentrations for fibroblasts and keratinocytes while 1.25 mg/mL amikacin and 0.034 mg/mL colistin do not affect fibroblasts’ viability and cytokine secretion but have an impact on keratinocytes. These concentrations are above the minimum inhibitory concentration but only amikacin could eradicate the biofilm. For the other antibiotics, cytotoxic concentrations are needed to eradicate the biofilm. Combinations with colistin at non-cytotoxic concentrations effectively eliminate the biofilm. These results provide information about the concentrations required when administering topical antibiotic treatments on skin lesions, and how these antibiotics affect wound management therapies. This study set the basis for the development of novel antibacterial wound healing strategies such as antibiotic artificial skin substitutes.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibiotics against Pseudomonas aeruginosa on Human Skin Cell Lines: Determination of the Highest Non-Cytotoxic Concentrations with Antibiofilm Capacity for Wound Healing Strategies

Quiñones-Vico,

Fernández-González,

Ubago-Rodríguez

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The carbapenem-resistant and bioluminescent strain PAO1 (CRPAO1) was cultured as previously described. 14 PA ATCC 27853 was used as a quality control strain.…”

Section: Methodsmentioning

confidence: 99%

The Potentiation Activity of Azithromycin in Combination with Colistin or Levofloxacin Against Pseudomonas aeruginosa Biofilm Infection

Wang,

Li,

Zhang

et al. 2024

IDR

Self Cite

View full text Add to dashboard Cite

Objective Pseudomonas aeruginosa (PA) often displays drug resistance and biofilm-mediated adaptability. Here, we aimed to evaluate the antibiofilm efficacy of azithromycin-based combination regimens. Methods Minimum inhibitory concentrations (MICs), minimal biofilm eradication concentrations (MBECs), and MBEC-combination of azithromycin, colistin, amikacin, and levofloxacin to bioluminescent strain PAO1 and carbapenem-resistant PAO1 (CRPAO1) were assessed. An animal biofilm infection model was established and detected using a live animal bio-photonic imaging system. Results In vitro, PAO1 and CRPAO1 were susceptible to colistin, amikacin, and levofloxacin, while they were unsusceptible to azithromycin. The combinations based on azithromycin have no synergistic effect on biofilm in vitro. In vivo, azithromycin plus colistin or levofloxacin could shorten the PAO1 biofilm eradication time, which totally eradicates the biofilm in all mice on the 8 th or 6 th day, while monotherapy only eradicate biofilm in 70% or 80% mice on the 8 th day. For CRPAO1 biofilm, only azithromycin–colistin combination and colistin monotherapy eradicated the bacteria in 60% and 40% of mice at the 6 th day. Conclusion Azithromycin-based combinations containing levofloxacin or colistin had no synergistic effect in vitro, and they are promising for clinical applications due to the good synergistic activity against PAO1 biofilms in vivo.

show abstract

The use of combination therapy for the improvement of colistin activity against bacterial biofilm

Jalil,

Alrawe,

Al-Saffar

et al. 2023

Braz J Microbiol

View full text Add to dashboard Cite

The Efficacy of Colistin Combined with Amikacin or Levofloxacin against Pseudomonas aeruginosa Biofilm Infection

Abstract: Infections associated with PA biofilm formation are extremely challenging. When monotherapy fails to achieve optimal efficacy, combination therapy becomes the last option.

Cited by 5 publications

References 28 publications

Antibiotics against Pseudomonas aeruginosa on Human Skin Cell Lines: Determination of the Highest Non-Cytotoxic Concentrations with Antibiofilm Capacity for Wound Healing Strategies

Antibiotics against Pseudomonas aeruginosa on Human Skin Cell Lines: Determination of the Highest Non-Cytotoxic Concentrations with Antibiofilm Capacity for Wound Healing Strategies

The Potentiation Activity of Azithromycin in Combination with Colistin or Levofloxacin Against Pseudomonas aeruginosa Biofilm Infection

The use of combination therapy for the improvement of colistin activity against bacterial biofilm

Contact Info

Product

Resources

About