The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion

Wang, He; Wang, Kun; Liu, Bo; Bian, Xiaoqian; Tan, Xiaojie; Jiang, Haitao

doi:10.1016/j.heliyon.2023.e15585

Cited by 2 publications

(2 citation statements)

References 43 publications

(52 reference statements)

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“…Wang et al 10.3389/fcimb.2023.1288666 Frontiers in Cellular and Infection Microbiology frontiersin.org lymphocytes predominate (approximately 80%), are key sites for assessing changes in intestinal immune function (Liu et al, 2021). For example, ischaemia-reperfusion-induced damage to the intestinal mucosal barrier has been associated with an increase in CD8+ T lymphocytes (Wang et al, 2023). Interleukin serves as an early inflammatory mediator, stimulating inflammatory responses, cell proliferation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury

Wang,

Zhang,

Zhang

et al. 2024

Front. Cell. Infect. Microbiol.

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IntroductionColorectal cancer (CRC) is the third most common malignant tumor, and neoadjuvant chemo-radiotherapy is usually recommended for advanced stage colorectal cancer. Radiotherapy can cause damage to intestinal mucosal barrier, which may be related to perioperative complications. Intestinal microbiota is one of the constituents of the intestinal mucosal biological barrier, and literature reports that patients with CRC have changes in corresponding oral microbiota. This study aims to analyze the levels of immunoglobulin SIgA, inflammatory factors, lymphocyte subsets quantity, and proportion in surgical specimens of intestinal mucosa at different time intervals after radiotherapy, in order to seek investigation for the optimal surgical time after radiotherapy and to provide evidence for finding probiotics or immunomodulators through high-throughput sequencing of bacterial 16s rRNA in patients' saliva microbiota. Ultimately, this may provide new ideas for reducing perioperative complications caused by radiotherapy-induced intestinal damage.MethodsWe selected intestinal mucosal tissue and saliva samples from over 40 patients in our center who did not undergo radiotherapy and underwent surgery at different time intervals after radiotherapy. Detection of SIgA was performed using ELISA assay. Western Blotting was used to detect IL-1β, IL-6, and IL-17 in the intestinal mucosal tissue. Flow cytometry was used to detect CD4 and CD8. And the microbial community changes in saliva samples were detected through 16s rRNA sequencing.ResultsAfter radiotherapy, changes in SIgA, various cytokines, CD4CD8 lymphocyte subsets, and oral microbiota in the intestinal mucosal tissue of rectal cancer patients may occur. Over time, this change may gradually recover.DiscussionIn colorectal cancer, oncological aspects often receive more attention, while studies focusing on the intestinal mucosal barrier are less common. This study aims to understand the repair mechanisms of the intestinal mucosal barrier and reduce complications arising from radiotherapy-induced damage. The relationship between oral microbiota and systemic diseases has gained interest in recent years. However, the literature on the oral microbiota after radiotherapy for rectal cancer remains scarce. This study addresses this gap by analysing changes in the salivary microbiota of rectal cancer patients before and after radiotherapy, shedding light on microbiota changes. It aims to lay the groundwork for identifying suitable probiotics or immunomodulators to alleviate perioperative complications and improve the prognosis of CRC.

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Section: Discussionmentioning

confidence: 99%

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury

Wang,

Zhang,

Zhang

et al. 2024

Front. Cell. Infect. Microbiol.

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“…As a prominent member of the stem cell family, MSCs find wide application in various clinical diseases ( 116 ). Research has demonstrated the protective effect of bone marrow-derived mesenchymal stem cells (BMSCs) on II/R-ALI ( 117 ). The mechanism primarily involves two categories: (1) exogenous MSCs migrate to locally damaged intestinal tissues and differentiate into IEC, thereby ensuring the integrity of the intestinal barrier, reducing bacterial translocation, and mitigating distal lung injury; (2) exogenous MSCs exert anti-inflammatory, anti-oxidative, anti-apoptotic effects, promote cell proliferation, and angiogenesis through the release of cytokines.…”

Section: Treatment Approachmentioning

confidence: 99%

Advancements in the study of acute lung injury resulting from intestinal ischemia/reperfusion

Lv,

Zhao,

et al. 2024

Front. Med.

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Intestinal ischemia/reperfusion is a prevalent pathological process that can result in intestinal dysfunction, bacterial translocation, energy metabolism disturbances, and subsequent harm to distal tissues and organs via the circulatory system. Acute lung injury frequently arises as a complication of intestinal ischemia/reperfusion, exhibiting early onset and a grim prognosis. Without appropriate preventative measures and efficacious interventions, this condition may progress to acute respiratory distress syndrome and elevate mortality rates. Nonetheless, the precise mechanisms and efficacious treatments remain elusive. This paper synthesizes recent research models and pertinent injury evaluation criteria within the realm of acute lung injury induced by intestinal ischemia/reperfusion. The objective is to investigate the roles of pathophysiological mechanisms like oxidative stress, inflammatory response, apoptosis, ferroptosis, and pyroptosis; and to assess the strengths and limitations of current therapeutic approaches for acute lung injury stemming from intestinal ischemia/reperfusion. The goal is to elucidate potential targets for enhancing recovery rates, identify suitable treatment modalities, and offer insights for translating fundamental research into clinical applications.

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The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion

Cited by 2 publications

References 43 publications

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury

Advancements in the study of acute lung injury resulting from intestinal ischemia/reperfusion

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