The efficacy and safety of heat‐killed Lactobacillus paracasei for treatment of perennial allergic rhinitis induced by house‐dust mite

Abstract

Live Lactobacillus paracasi 33 (LP33) may effectively improve the quality of life for patients with perennial allergic rhinitis. It has been demonstrated that heat-killed lactic acid bacteria (LAB) suppress specific immunoglobulin E synthesis and stimulate interleukin-12 production in animals. The aim of this study was, therefore, to evaluate the efficacy of heat-killed LP33 in the treatment of allergic rhinitis induced by house-dust-mite in human subjects. A total of 90 patients were enrolled in a randomized, double blind, placebo-controlled trial and assigned to three treatment groups. Patients in groups A and B received two capsules per day of live or heat-killed LAB (5 x 10(9) colony-forming units/capsule), respectively, over a period of 30 days while those in Group C received placebo capsules. A modified questionnaire on pediatric rhinoconjunctivitis-related quality of life was administered to all subjects or their parents during each clinical visit. The overall quality of life score decreased for groups A and B, as compared with the placebo group, in terms of both frequency (9.47 +/- 2.89, 6.30 +/- 2.19, vs. -3.47 +/- 1.53, respectively; p < 0.0001) and level of bother (5.91 +/- 3.21, 6.04 +/- 2.44, vs. -2.80 +/- 1.64, respectively; p = 0.004) after the 30-day treatment. The efficacy of the heat-killed LP33 was not inferior to the live variant. No obvious side effects were reported for either active treatment group during the study period. Our results suggest that heat-killed LP33 can effectively improve the overall quality of life for patients with allergic rhinitis, and that it may be efficacious as an alternative treatment.

Pediatric Allergic Rhinitis and Asthma: Can the March be Halted?

“…Some controlled trials reported overall positive results with the administration of lactobacillus in children with AR [108,109], and a small study reported clinical and anti-inflammatory effects provided by Bacillus clausii [110]. Data showing that probiotic supplementation modulates immune responses in AR and may potentially alleviate the AR symptom severity [111] are encouraging, but their primary preventive effect on atopic diseases, probably by enhancing the T helper type 1 (TH1) response, is still unconfirmed [112].…”

Suppressive Effects of Bifidobacterium breve Strain M-16V on T-Helper Type 2 Immune Responses in a Murine Model

“…The organisms were lyophilized and suspended in PBS at 10 mg/ml and then killed by heating the solution to 100°C for 30 min. The stock suspensions which contained approximately 2ϫ10 10 , 1ϫ10 10 , and 3ϫ10 10 cells/ml for M-16V, LGG and STH, respectively, The Bifidobacterium breve M-16V strain has previously been shown to be effective in infants in improving the symptoms of allergic hypersensitivity to cow's milk and atopic dermatitis. In the current study, we investigated the effect of an oral administration of M-16V on immunoglobulin (Ig) E production in BALB/c mice.…”

“…In numerous human trials, the intake of probiotics, such as lactobacilli and bifidobacteria, has been reported to prevent several allergic diseases. [6][7][8][9][10][11] The potential benefit of probiotic bacteria in the modulation and regulation of the immune response has also been reported in both in vitro and in animal studies. [12][13][14][15][16][17][18][19] Bifidobacterium breve M-16V is a probiotic strain that was isolated from fecal sample of a healthy baby.…”

Potential of Selected Strains of Lactic Acid Bacteria to Induce a Th1 Immune Profile

“…9) Moreover, it has been proposed that DCs localized in the gut-associated lymphoid tissue can sample bacteria directly from the intestinal lumen and modify the immune homeostasis. 10) Clinical studies conducted in humans have indicated that LAB can alleviate the symptoms of atopy [11][12][13][14] and perennial allergic rhinitis [15][16][17] and enhance the anti-carcinogenic activity of the immune system. [18][19][20][21] Since the bacterial properties required for the different applications are clearly distinct, selection of the LAB strain is very important for a more rational use of these organisms.…”