The efficacy and potential mechanism of cnidium lactone to inhibit osteoclast differentiation

Liang, Junyang; Wu, Wenliang; Chen, Yunxia; Liu, Haichun

doi:10.1080/21691401.2019.1637881

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…In addition, studies have shown that garcinol can reduce RANKL-induced osteoclastogenesis by regulating the PI3K/AKT/mTOR signaling pathway and the downstream factor c-FOS [ 40 ]. Cnidium lactone regulated osteoporosis by regulating c-FOS/NFATc1 signaling pathways through p38 MAPK and PI3K-Akt [ 41 ]. Further, we investigated its effect on MC3T3-E1 cells by overexpressing FOS.…”

Section: Discussionmentioning

confidence: 99%

Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology

Zhu

Linhua

et al. 2023

J Orthop Surg Res

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Background Liuwei Dihuang Pill (LP) was verified to alleviate postmenopausal osteoporosis (PMOP) development. Nevertheless, the major constituent of LP and the related network pharmacology study remain unexplored. Methods Protein–protein interaction was established to identify the downstream target of LP in PMOP, and the related signaling pathway was investigated by bioinformatics analysis. MC3T3-E1 cells were added to ferric ammonium citrate (FAC) to mimic osteoporosis in vitro. The osteoblasts were identified by Alizarin red staining. Western blot was applied to evaluate protein levels. In addition, Cell Counting Kit-8 (CCK8) assay was applied to assess cell viability, and cell apoptosis was assessed by flow cytometry. Results Quercetin was the major constituent of LP. In addition, quercetin significantly reversed FAC-induced inhibition of osteogenic differentiation in MC3T3-E1 cells. In addition, quercetin notably abolished the FAC-induced upregulation of Bax, Caspase-3, FOS, JUN, TGFB1 and PPARD. In contrast, Bcl-2, p-mTOR/mTOR, p-AKT/AKT and p-PI3K/PI3K levels in MC3T3-E1 cells were reduced by FAC, which was restored by quercetin. Meanwhile, FAC notably inhibited the viability of MC3T3-E1 cells via inducing apoptosis, but this impact was abolished by quercetin. Furthermore, quercetin could reverse pcDNA3.1-FOS-mediated growth of FAC-treated osteoblasts by mediating PI3K/AKT/mTOR signaling. Conclusion Quercetin alleviated the progression of PMOP via activation of PI3K/AKT/mTOR signaling. Hence, this study would shed novel insights into discovering new methods against PMOP.

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Section: Discussionmentioning

confidence: 99%

Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology

Zhu

Linhua

et al. 2023

J Orthop Surg Res

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“…The murine macrophage cell line RAW 264.7 was used in the current study as it is widely used in osteoclastic experiments (Liang et al, 2019;Zeng et al, 2019). RAW 264.7 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).…”

Section: Cell Linesmentioning

confidence: 99%

Protective Effects of Punicalagin on Osteoporosis by Inhibiting Osteoclastogenesis and Inflammation via the NF-κB and MAPK Pathways

et al. 2020

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Postmenopausal osteoporosis is a worldwide disease characterized by reduced bone mineral density and increased fracture risk. Inflammatory bone loss due to excessive osteoclast bone resorption is significant in the pathogenesis and development of osteoporosis. Punicalagin (PUN) is a pomegranate fruit derivative and has potential antiinflammatory effects. However, the effect of PUN on osteoporotic bone loss has yet to be clarified. In this study, we investigated the effect of PUN on RANKL-induced osteoclast formation and bone resorption in vitro, as well as its potential therapeutic effect on ovariectomized-induced bone loss in vivo. PUN was demonstrated to suppress osteoclast formation and bone resorptive function dose-dependently, while osteoclastspecific genes were also downregulated by PUN. In vivo micro-CT and histopathological staining showed that the OVX procedure led to significant bone loss characterized by decreased bone parameters and increased osteoclast numbers, while PUN treatment dramatically prevented these changes. Furthermore, PUN treatment effectively inhibited proinflammatory cytokine expression in vitro. Mechanistically, PUN maintained bone mass via suppressing nuclear factor kB (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathway activation. Collectively, our observations provide evidence that PUN is a potential candidate for the treatment of osteoporosis.

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“…Cnidium lactone is a potent herbal remedy that can prevent bone loss in a model of ovariectomyinduced bone loss in rats (Wang et al, 2020). Liang et al (2019) found in a study of RANKL-induced differentiation of RAW264.7 cells that Cnidium lactone could inhibit the differentiation of TRAP-positive multinucleated osteoclasts and reduce the bone resorption of osteoclasts. In molecular mechanism, Cnidium lactone is able to inhibit the expression of c-Fos and NFATc1, the main transcription factors of osteoclast differentiation, by inhibiting the levels of phosphorylation of p38 and AKT and reducing the expression of p38 MAPK and PI3K-Akt signaling pathways.…”

Section: Natural Plant Derivatives and Compounds Modulate The Akt Sig...mentioning

confidence: 99%

Receptor activator of nuclear factor-κB ligand-mediated osteoclastogenesis signaling pathway and related therapeutic natural compounds

Zhang

Kong

et al. 2022

Front. Pharmacol.

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Osteoclast is a hematopoietic precursor cell derived from the mononuclear macrophage cell line, which is the only cell with bone resorption function. Its abnormal activation can cause serious osteolysis related diseases such as rheumatoid arthritis, Paget’s disease and osteoporosis. In recent years, the adverse effects caused by anabolic anti-osteolytic drugs have increased the interest of researchers in the potential therapeutic and preventive effects of natural plant derivatives and natural compounds against osteolytic diseases caused by osteoclasts. Natural plant derivatives and natural compounds have become major research hotspots for the treatment of osteolysis-related diseases due to their good safety profile and ability to improve bone. This paper provides an overview of recent advances in the molecular mechanisms of RANKL and downstream signaling pathways in osteoclast differentiation, and briefly outlines potential natural compounds with antiosteoclast activity and molecular mechanisms.

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The efficacy and potential mechanism of cnidium lactone to inhibit osteoclast differentiation

Cited by 6 publications

References 19 publications

Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology

Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology

Protective Effects of Punicalagin on Osteoporosis by Inhibiting Osteoclastogenesis and Inflammation via the NF-κB and MAPK Pathways

Receptor activator of nuclear factor-κB ligand-mediated osteoclastogenesis signaling pathway and related therapeutic natural compounds

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