The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio)

Küçükoğlu, Melek; Binokay, Uğur Seçil; Pekmezekmek, Ayper Boğa

doi:10.3906/biy-1203-27

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…Meanwhile, embryos in batik liquid waste treatment above 5% did not hatch. Slow developments due to exposure to toxicants, as suggested by Küçükoǧlu et al (2013), on zebrafish (D. rerio) embryos on exposure to zinc chloride began to hatch on the 7 th day after fertilization, compared to the control embryos hatched on the 4 th day after fertilization. Moreover, Nisha et al (2020) found that toxic material (chromium) in water caused abnormalities in zebrafish embryos, such as eye and tail development.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in the shape of the vertebrae can characterize the form of larval abnormalities. Researcher Küçükoǧlu et al (2013) informed that one of the mechanisms that cause vertebral damage is disruption during early vertebral development caused by inhibition of collagen synthesis. Toxic substances contained in batik liquid waste inhibited collagen synthesis during the development of vertebrae of Nilem Fish larvae during the study.…”

Section: Discussionmentioning

confidence: 99%

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Embryo and larvae development of Nilem Fish, Osteochilus vittatus reared in batik liquid waste

NUGRAHESTHI,

WIJAYANTI,

HABIBAH

2023

Nusantara Biosci

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Abstract. Nugrahesthi GH, Wijayanti GE, Habibah AN. 2023. Embryo and larvae development of Nilem Fish, Osteochilus vittatus reared in batik liquid waste. Nusantara Bioscience 15: 105-112. The embryonic and larval stages are critical phases of an organism's development. For aquatic organisms, development is affected by environmental factors such as liquid waste. A batik liquid waste is a waste product of the textile industry usually streamed directly into the aquatic environment. This study aimed to observe the effects of exposure to batik liquid waste effects on developing Nilem (Osteochilus vittatus Valenciennes, 1842) fish embryos and larvae. The research was conducted using a completely randomized design. One hundred two-celled embryos were kept in batik liquid waste with dilution concentrations of 0% (the control), 5%, 10%, 15%, and 20% of water until the age of 4 days after hatching with five replications. The time of embryo evaluation was 60th minutes, 120th minutes, and 180th minutes after fertilization; the larval stage evaluation time was the 24th, 48th, 72nd, and 96th hour after fertilization; five embryos were evaluated for each replication. The results showed that embryo exposure to batik liquid waste affected the height of the blastoderm embryo, accumulation of waste in the chorion of the embryo, deceleration of embryonic development, increased larval abnormalities, decreased the survival rate of larvae, and acceleration of yolk absorption of fish larvae. Embryos were successfully hatched and produced larvae only in the control and 5% batik liquid waste medium. Batik liquid waste interfered with O. vittatus embryo development and generated mortality above 5%.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Embryo and larvae development of Nilem Fish, Osteochilus vittatus reared in batik liquid waste

NUGRAHESTHI,

WIJAYANTI,

HABIBAH

2023

Nusantara Biosci

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Interference with zinc homeostasis and oxidative stress induction as probable mechanisms for cadmium-induced embryo-toxicity in zebrafish

Chouchene

Kessabi

Guéguen

et al. 2022

Environ Sci Pollut Res

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Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users

et al. 2023

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Despite the increasing exploitation of nanomaterials (NMs) in an array of consumer products, there are uncertainties regarding their potential adverse impact on human health. Investigation of whether NMs activate a pro-inflammatory response is routinely used to assess their toxicity in in vitro and in vivo (rodent) studies. The use of zebrafish (Danio rerio) to investigate inflammatory responses to chemicals, pathogens and injury has increased considerably over recent years. Zebrafish have also been used to investigate the role of inflammation in disease pathogenesis and for drug discovery. Availability of transgenic strains which express fluorescent proteins in immune cells (e.g. macrophages and neutrophils) enables the visualization and quantification of immune cell accumulation in the target site(s) of interest. We therefore propose that transgenic zebrafish have great utility for screening the toxicity of NMs via investigation of inflammatory responses. Indeed, we have successfully used non-protected life stages of transgenic zebrafish with fluorescent neutrophils (Tg(mpx:EGFP114) to investigate inflammatory responses to NMs. The more widespread use of transgenic zebrafish in nanotoxicology could reduce the reliance placed on rodents and thereby enhance the implementation of the 3Rs principles. As zebrafish continue to grow in popularity it is timely to offer guidance to new users on their use. Here we will reflect on: exposure routes that can adopted to mimic human/rodent exposure, what transgenic strains and life stages are best suited to investigate inflammatory responses, selection criteria for zebrafish embryos/larvae, the inclusion of appropriate controls, the importance of dose selection and sample size, and how the (inflammatory) response can be quantified. It is hoped that our recommendations will support the development of standard protocols that can be used to assess whether NMs activate inflammatory responses. Importantly, the themes discussed are not restricted to NMs but relevant also to zebrafish application in ecotoxicology or human health focused studies.

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The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio)

Cited by 8 publications

References 29 publications

Embryo and larvae development of Nilem Fish, Osteochilus vittatus reared in batik liquid waste

Embryo and larvae development of Nilem Fish, Osteochilus vittatus reared in batik liquid waste

Interference with zinc homeostasis and oxidative stress induction as probable mechanisms for cadmium-induced embryo-toxicity in zebrafish

Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users

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