The Effects of Yunjogyosim-tang on Stress and Sleep-related Substance of Rats Induced by Chronic Mild Stress

Abstract

Objectives: The study aims were to evaluate the Anti-stress and Sleep-inductive effects of Yunjogyosim-tang (YJGS) in rats induced by Chronic Mild Stress (CMS). Methods: 25 healthy Rats were randomly divided into 5 groups; Normal, CMS (Control), YJGS 50, YJGS 100, and YJGS 200. All rats except the normal group were exposed to random stress conditions like water deprivation, empty bottles, forced runs on the treadmill, etc. according to the timetable of CSM for 3 weeks. After a week starting the experiment, Rats in YJGS 50, YJGS 100, and YJGS 200 groups were fed orally once a day for 2 weeks. Blood samples were taken afterward from each rat for analysis of Complete blood count, AST, ALT, and glucose levels. Corticosterone, serotonin, and melatonin were measured by ELISA kit. BDNF and TNF-α were measured by RT-PCT. Results: 1. Corticosterone levels were lower in YJGS 50, and YJGS 200 groups compared to the control group. 2. Serotonin levels were lower in the YJGS 100 and YJGS 200 groups compared to the control group. 3. Melatonin levels were higher in the YJGS 50 group compared to the control group. 4. Activity levels were higher in the BDNF, and YJGS 50 groups compared to the control group. 5. In the Activity of TNF-α , YJGS 50 group showed a significant decrease compared to the control group. 6. In the Glucose content, YJGS 50, YJGS 100, and YJGS 200 groups showed a significant decrease compared to the control group. 7. When analyzing leukocytes, monocytes were found to be increased in YJGS 100 and YJGS 200 groups compared to the control group. 8. When analyzing red blood cells, more changes occurred to erythrocytes and thrombocytes in the YJGS 50 and YJGS 200 groups than in the control group. Hemoglobin was significantly increased in the YJGS 100 group and platelet levels were significantly increased in all experimental groups compared with the control group. Conclusions: These results suggest that Yunjogyosim-tang has anti-stress and sleep-inductive effects on rats induced by CMS.

Effects of Banhabaekchulcheonma-Tang on Brain Injury and Cognitive Function Impairment Caused by Bilateral Common Carotid Artery Stenosis in a Mouse Model

“…This difference in results may be due to differences in the surgical procedure and the degree of brain damage caused by ischemia. Additionally, in a study [13] using MCAO model rats, BBCT administration improved working memory deficits in an 8-arm radial maze. The types of rodents, cerebral ischemia models, and mazes used to evaluate working memory function may have contributed to this difference.…”

“…Moreover, BBCT has been reported to possess antihypertensive, anti-oxidant, and cardioprotective effects by reducing IL-1, IL-6, inducible nitric oxide synthase, and tumor necrosis factor-α (TNF-α) levels and suppressing the activity of the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) pathway in hypertensive rats [10,18]. In MCAO-induced VD model rats, BBCT improved cognitive dysfunction in an 8-arm radial maze task, exhibited neuroprotective effects, and increased acetylcholinesterase in the hippocampus [13]. Among the components of BBCT, gastrodin of Gastrodiae Rhizoma attenuated amyloid-β (Aβ) deposition and glial activation in the brain of Alzheimer's disease model mice and inhibited abnormal phosphorylation of Aβ and Tau in the hippocampus of VD model rats [19,20].…”

“…In clinical studies, BBCT was found to improve the activities of daily living in patients with cerebral infarction [11], and cognitive function in patients with VD [12]. A study on a rat model of middle cerebral artery occlusion (MCAO) revealed that BBCT had neuroprotective effects on hippocampal neurons and increased acetylcholinesterase expression [13].…”