The Effects of Wound Lavage Solutions on Canine Fibroblasts: An In Vitro Study

Buffa, Eugene A.; Lubbe, A. M.; Verstraete, Frank J.M.; Swaim, Steven F.

doi:10.1111/j.1532-950x.1997.tb00517.x

Cited by 52 publications

(14 citation statements)

References 11 publications

(3 reference statements)

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“…Physiological saline showed better wound healing effect than non-physiological tap water and an antiseptic in this study as is shown in table two. This corroborates the work of Buffa et al (1997) who showed that canine fibroblast had more damage with tap water than with normal saline. Other workers have documented this effect of normal saline on healing wounds but the mechanism of this effect is presently not known (Jacobson and Lineaweaver et al, 1985).…”

Section: Discussionsupporting

confidence: 91%

A Comparison of the Effect of Chlorhexidine, Tap Water and Normal Saline on Healing Wounds

2006

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Many researchers have advocated in recent times that antiseptic use in healing wounds should be discouraged. Antiseptics have been found to retard healing of wounds. Innocuous solutions like normal saline and tap water are being considered as better alternatives to antiseptics. Chlorhexidine, a commonly used antiseptic, is known to be less toxic on granulation cells. We decided to compare the effect of chlorhexidine, normal saline and tap water on healing wounds. Three groups of wistar rats had 2 by 2cm full thickness wounds made on their right dorsolateral flanks. The wounds were dressed with either chlorhexidine, normal saline or tap water. Wounds were examined at three-day intervals and measurements of area covered were taken on the first day of study and the ninth day. Wound contraction on the ninth day and number of days for healing to take place in the different groups was taken and statistical analysis using student t-test was done to compare the values. Gross morphology of the wounds was also observed. The result showed an inhibitory effect of chlorhexidine on healing wounds. The wound contraction in the antiseptic group was less than the tap water and saline group on day nine. The average number of days for wound healing to be completed in the antiseptic group was more than the other two. These results were statistically significant when compared with the other two groups. There was no statistical difference in the wound contraction values and rate of healing of the saline and tap water dressed wounds. Grossly, all the wounds dressed with antiseptic also had greenish exudates on their surface by day nine with pale looking granulation tissue, and there was greater mortality in this group.

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Section: Discussionsupporting

confidence: 91%

A Comparison of the Effect of Chlorhexidine, Tap Water and Normal Saline on Healing Wounds

2006

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“…There appears to be growing evidence to suggest that a completely sterile wound is at a disadvantage (Inflammation and Wound Healing, 2004), supporting the concept that physical removal to a level below the threshold for infection (around 10 5 bacteria per cm 2 depending upon virulence) is our goal. Removal of excessive bacterial burden rather than complete annihilation using a toxic solution would appear to be the preferential approach (Buffa et al, 1997).…”

Section: Back To the Dark Ages: Cleansing Wounds From The Sensible Tomentioning

confidence: 99%

Wound cleansing: Have we learned anything?

Hollis

2012

Companion Animal

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: Wound cleansing has been practised for centuries and despite our advances in the medical field there is still much confusion about which solution to use for cleansing wounds in practice. From boiled water to cytotoxic chemicals the annihilation of bacteria has become our biggest obsession but it should not be at the expense of the healing process. As multi‐resistant organisms begin to reduce our options for antibiotic use it appears that dilution remains the solution to pollution.

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“…Lavage [11][12][13] Die Wundspülung ist ein wichtiger Aspekt der Wundbehandlung. Sie hilft, die bakterielle Kontamination zu verringern und entfernt Fremdkörper sowie anderes loses Material aus der Wunde.…”

Section: Offenes Wundmanagementunclassified

“…Die Spülung sollte mit körperwarmer Ringer-Lösung oder Polyhexaniden (Prontovet ® ) durchgeführt werden. Eine In-vivo-Studie konnte zeigen, dass Leitungswasser und sterile Kochsalzlösung zytotoxisch für Fibroblasten sind [11]. Antiseptika sollten vermieden werden, da sie ebenfalls zytotoxisch für das Wundbett sind und den Heilungsprozess behindern [12].…”

Section: Offenes Wundmanagementunclassified