The following article describes a case study about decontamination of a 65-room new animal research facility located in the Northeast. The decontamination took place during the cold winter month of January, and all equipment used to run the facility was in place prior to beginning the decontamination. This facility had an essential need for complete decontamination because a lot of its equipment was procured from other facilities and crosscontamination was a concern. Chlorine dioxide gas was used due to the inherent properties of a gas, such as excellent distribution and penetration which were required due to the numerous rooms in the facility. The target concentration was not reached, but since photometric measurement was utilized, the exposure was extended ac cordingly and the end result was successful. All biological indicators were eradicated, and no residues and no material degradation were observed.
Facility DescriptionThe end-user of a new 65-room, 18,000 sq ft (180,000 cu ft / 5097 cu m) life science research facility required decontamination prior to the opening and occupying of the facility. The entire area was to be decontaminated prior to moving in the animals, since some of the equipment was previously used at another facility and the possibility of cross-contamination needed to be eliminated.Chlorine dioxide gas was used to fully decontaminate the area. The facility contained a variety of rooms such as a chemistry lab, animal holding rooms, procedure rooms, a cage wash room, bathrooms/showers, etc. No offices were located within the decontamination area. All the equipment needed to run the facility was in place prior to the decontamination. Various types of equipment, such as rodent racks and cages, bedding changing stations, biological safety cabinets, various plastics (for example, cages and water bottles), microscopes, video cameras, circuit-breaker panels, bathrooms and showers, smoke detectors, temperature and relative humidity (RH) sensors, as well as various analytical and electronic equipment used in a typical chemistry lab, were stored within the facility. All surfaces in the area were clean and non-porous, including stainless steel, epoxy-painted walls, solid flooring, painted steel cabinets, and plastic light fixtures. The facility was cleaned prior to the decontamination.
Background Information: Determine What Method to UseTo perform a facility decontamination, a choice had to be made to determine which decontamination agent to utilize. In this case three were considered: formaldehyde gas, vapor phase hydrogen peroxide (VPHP), and chlorine dioxide (CD) gas. All were known to be effective decontaminants for spore and non-spore forming bacteria under ideal laboratory conditions (i.e., clean flat surfaces lacking porous materials or potential dead-legs with which fumigant penetration might be retarded).
Formaldehyde GasFormaldehyde gas is the agent that is probably used most frequently when compared to CD gas and VPHP . It is used most often because it is effective (gets good kill)...