The effects of vitamin E on NK cell activity and lymphocyte proliferation in treated mice by 2,3,7,8-tetrachlorodibenzo-p-dioxin

Abstract

The study was conducted to investigate the effects of vitamin E on NK cell activity and lymphocyte proliferation in tr eated female mice by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). In chronic TCDD trial, 45 mice were divided into 5 groups, and the levels of TCDD and vitamin E were 0 and 0, 100 and 0, 100 and 20, 100 and 100, and 100 ng/kg/d and 500 mg/kg/d, respectively. In acute TCDD trial, 24 mice were divided into three groups, and the levels of TCDD and vitamin E were 0 and 0, 30 and 0, and 30 microg/kg and 100 mg/kg, respectively. The results showed chronic TCDD-treatment caused decrease tendencies of spleen NK cell activity and lymphocyte proliferation, and vitamin E 100mg/kg alleviated the decreases tendencies caused by chronic TCDD-treatment, and the lymphocyte proliferation in the group given vitamin E 100mg/kg was significantly higher than that of the chronic TCDD-treated group. Acute TCDD-treatment suppressed the NK cell activity and lymphocyte proliferation, and vitamin E 100mg/kg significantly alleviated the decrease caused by acute TCDD-treatment. These results suggested that TCDD resulted in immunotoxicity, and the toxicity of acute TCDD-treatment was severe compared with chronic TCDD, while vitamin E alleviated the immunotoxicity from TCDD.

Effect of tocopherol on biochemical blood parameters in pleuritis-induced rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin

“…An observed increase in the IL-6 level at the 24th hour of inflammation in group treated with TCDD 3 weeks before the pleuritis injection (group A) and decrease in the IL-6 level at the 24th hour of inflammation in group treated with TCDD 3 weeks before the pleuritis initiation and daily supplementation with tocopherol (group B) suggest the anti-inflammation vitamin E action in an early phase of the inflammation. Other studies prove that tocopherol application for a few weeks can also drop oxidative stress (Ahmad et al, 2005;Norazlina et al, 2007;Wang et al, 2009). Our results show that daily tocopherol application in rats within 3 weeks before the pleuritis initiation contributes to the significant drop in the IL-1, IL-6 and TNF concentrations during the inflammation.…”

Insight into innate immune response in “Yusho”: The impact of natural killer cell and regulatory T cell on inflammatory prone diathesis of Yusho patients

“…In a previous study of murine NK cells, activation of AhR using the endogenous tryptophan derivative 6-formylindolo[3,2-b]carbazole (FICZ) promoted IFN-γ production, whereas AhR-deficient NK cells had reduced cytolytic activity (Shin et al, 2013). Conversely, some previous studies reported that AhR activation suppressed NK cell activation (Wang et al, 2009). In the current study, the proportion of NK cells was significantly higher in Yusho patients than in healthy controls, which contrasts with the results of a study of adolescents in Flanders, which found that the percentage of NK cells in blood was negatively correlated with serum PCB concentration (Van Den Heuvel et al, 2002).…”