The effects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specific for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occurring canine transmissible venereal tumor

ÖZYİĞİT, Musa Özgür; NAK, Deniz; AKŞİT, Hasan; ÖZTÜRKOĞLU, Sevda İNAN; ŞİMŞEK, Gözde; UZABACI, Ender; NAK, Yavuz; SEYREK, Kamil

doi:10.3906/vet-1311-46

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…Lokesh et al 2014 reported that they could not detect the Bcl-2 reaction in TVT cases. Similar to previous studies (Özyiğit et al, 2014;Setthawongsin et al, 2019), we observed that Bcl-2 expression was more severe in progressive TVT cases and Bax expression was more severe in regressive TVT cases.…”

Section: Discussionsupporting

confidence: 92%

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Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

Karakurt

Kuru

Dağ

et al. 2020

Journal of Istanbul Veterinary Sciences

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In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin & Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells.

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Section: Discussionsupporting

confidence: 92%

“…Transmissible venereal tumor (TVT) also knowns as infectious sarcoma, venereal granuloma, transmissible lymphosarcoma or Sticker tumor, is the most common genital tumor in dogs (Özyiğit et. al., 2014;Paramjeet et.…”

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confidence: 99%

Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

Karakurt

Kuru

Dağ

et al. 2020

Journal of Istanbul Veterinary Sciences

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In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line

et al. 2022

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The canine transmissible venereal tumor (CTVT) is the most common malignity in dogs. Because there are reports that this tumor is resistant to vincristine sulfate, the chemotherapeutic options are scarce, and the development of new therapeutic approaches is necessary. In this study, we evaluated the cytotoxic activity of vincristine, doxorubicin, temozolomide, panobinostat, toceranib, gemcitabine, cisplatin, fluorouracil, cyclophosphamide, and methotrexate on a CTVT cell line, determining that all drugs decreased the viability in a dose-dependent manner. Furthermore, they inhibit cellular migration in a time- and drug-dependent manner, as evaluated by the wound healing assay. On the other hand, vincristine, panobinostat, gemcitabine, toceranib, cyclophosphamide, and methotrexate increased the percentage of cells in the subG1 phase, and doxorubicin, temozolomide, gemcitabine, toceranib, and methotrexate decreased the percentage of cells in the synthesis phase. To efficientize the use of vincristine, only toceranib increased the cytotoxic effect of vincristine in a synergistic manner. Our results confirm the use of vincristine as the gold standard for CTVT treatment as monotherapy and suggest the use of a combinatorial and sequential treatment with toceranib.

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The effects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specific for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occurring canine transmissible venereal tumor

Cited by 2 publications

References 23 publications

Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line

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