The effects of verapamil and heparin co‐administration on sperm parameters and oxidative stress in prevention of testicular torsion/detorsion damage in rats

Abstract

In this research, the impacts of combined administration of verapamil and heparin on testicular torsion damage were examined. In this experimental study, 30 sexually mature male Wistar albino rats were divided into five equal groups haphazardly (n = 6): Group 1 was the sham group. In group 2, a 2‐hr testicular torsion was induced, and thereafter, detorsion was done. Rats in group 3 and group 4 experienced an identical surgical procedure like group 2, but verapamil and heparin were administered in 0.3 mg/kg and 800 IU/kg doses respectively, and in group 5, a combination of verapamil and heparin were administered. Intraperitoneal drug injection in all treatment groups was done 30 min before testicular detorsion. Testicular torsion significantly changed sperm parameters, oxidative stress biomarkers and Cosentino's histological score compared to the sham group (p < .05). All treatment groups reduced testicular damage by decreasing oxidative stress and improving sperm parameters, but heparin and co‐administration of verapamil and heparin were significantly better than verapamil injection alone. However, heparin injected group was more effective than other treatment groups (p < .05). Overall, an anticoagulant like heparin is more effective than a calcium channel blocker such as verapamil, and it is more likely to reduce testicular torsion injuries.

Investigation of the protective role of chrysin within the framework of oxidative and inflammatory markers in experimental testicular ischaemia/reperfusion injury in rats

“…In our study, CAT values increased in the torsion/detorsion group compared to the control group, but this increase was not statistically significant. Our GSH findings support previous study results (Davoodi et al., 2020; Moradi‐Ozarlou et al., 2020; Sekerci et al., 2017). Chrysin, which we believe has therapeutic power, increased GSH and CAT values in T‐2h/D‐24h + CR group compared to T‐2h/D‐24h group.…”

“…A decrease in catalase and GSH levels has been reported in many of the torsion/detorsion studies (Davoodi, Raisi, Rajabzadeh, Hablolvarid, & Zakian, 2020; Moradi‐Ozarlou et al., 2020; Sekerci et al., 2017). In our study, CAT values increased in the torsion/detorsion group compared to the control group, but this increase was not statistically significant.…”

Co‐administration ofSalvia miltiorrhizaand verapamil inhibits detrimental effects of torsion/detorsion on testicular tissue in rats

“…In an experiment on the protective effects of Salvia miltiorrhiza extract on renal ischaemia–reperfusion, it was concluded that this herbal remedy could significantly reduce MDA and increase levels of GPx, GSH, CAT and SOD (Chen et al., 2012). In a study conducted by Davoodi, Raisi, et al (2020) regarding the verapamil and heparin co‐administration effects on the prevention of testicular torsion/detorsion injury, it was found that the combination of verapamil and heparin could significantly reduce oxidative stress and detrimental effects of testicular torsion (Davoodi, Raisi, et al, 2020). In our previous research on the effects of Salvia hydroalcoholic extract on testicular TD damage, results indicated that SM administration before detorsion significantly decreased MDA and increased GPx, CAT and TAC levels (Davoodi, Taheri, et al, 2020).…”

“…Herein, in the TD group, the level of MDA was increased considerably and levels of GPx, CAT and TAC, decreased. Pretreatment with SMV could noticeably increase the levels of GPx, CAT and TAC (Chen et al., 2012; Davoodi, Raisi, et al, 2020; Davoodi, Taheri, et al, 2020; Sertkaya et al., 2014).…”

“…In view of the susceptibility of sperm cells to ischaemia/reperfusion injury, numerous studies have evaluated sperm parameters following testicular T/D. It has been concluded that verapamil is not effective in improving sperm quality and maintaining its normal characteristics following I/R (Davoodi, Raisi, et al, 2020; Juneja et al., 1990; Moger, 1983). In a study conducted in 2018, it was found that verapamil has no concrete effect on sperm quality (Ameli et al., 2018).…”