The Effects of Varying Degrees of Allograft Decalcification on Cultured Porcine Osteoclast Cells

Abstract

In this in vitro model, porcine osteoclasts show significantly more resorptive activity as measured on calcium phosphate-coated disks in the presence of 2.41% residual calcium in DFDBA than in other DFDBA residual calcium levels.

Principles of demineralization: Modern strategies for the isolation of organic frameworks

“…In conclusion, demineralization is an ongoing, challenging group of mechanisms and aspects applicable and used by different scientific disciplines with their diverse directions including the following: evolutionary paleontology (Gould, 1970); astrobiology (McLoughlin et al, 2007); biomolecular deterioration and survival of organic matter (Collins et al, 2002); geomycology (Gadd, 2007); paleoclimatology (Schöne and Giere, 2005); seawater chemistry (Porter, 2007); material chemistry (Dujardin and Mann, 2002); geomedicine (Sahai, 2005); paleobathymetry (Wisshak and Rüggeberg, 2006); exobiology (Onofri et al, 2004); osteoarchaeology (Davis, 1997); archaeology and bioarcheology (Larsen, 2002); histology and histotechnology (Callis and Sterchi, 1998); forensic dentistry and forensic science (Rogers, 1988); demineralized bone allografts (Herold et al, 2002); dental anthropology (Alt et al, 1998); mineral dissolution (Nancollas, 1982;Wang et al, 2005bWang et al, , 2006a; bio-and chemical weathering (Benzerara et al, 2005); medical geology (Skinner and Berger, 2003); calcibiocavitology and endolithic microborings (Carriker and Smith, 1969); bioerosion (Lazar and Loya, 1991;Garcia-Pichel, 2006); remineralization of biomaterials ; shell repair and regeneration (Meenakshi et al, 1974;Palmer, 1983); sclerochronological studies ; bioremediation (Singh and Ward, 2004); bone diseases, osteoporosis, osteopenia, osteoclasia (Skinner, 2000); biodeterioration (Morton, 1987); taphonomy (Child, 1995); archeological bone chemistry (Pate, 1994); paleohistory, paleoecology, paleontology (Pate, 1994;…”

Nicotine Stimulates Osteoclast Resorption in a Porcine Marrow Cell Model

“…# Porcine marrow cell cultures were established according to modifications of previously published procedures. 25,26 S-DMEM contained Dulbecco's modified Eagle medium without phenol red, sodium pyruvate, or sodium bicarbonate; 1 × MEM non-essential amino acids; 10% heat-inactivated fetal bovine serum (FBS);** 1 × 10 −8 M 1,25-dihydroxy vitamin-D 3 ; † † 100 U penicillin and 0.1 mg streptomycin per ml; 50 µg/ml gentamicin; 1.25 µg/ml fungizone; 0.38% sodium bicarbonate;** 15 mM HEPES;** 0.11 mg/ml sodium pyruvate;** and 10 −5 M β-mercaptoethanol. ** The suspension of bone fragments and marrow was agitated for 1 to 2 minutes to aid in the detachment and suspension of the cells.…”

“…24 The well-established and high-yielding porcine marrow cell culture was selected to model osteoclast/odontoclast activity in this study. 25,26 The goal of this study was to evaluate the effects of nicotine concentrations, relevant to tobacco use, on osteoclastic activity, namely bone resorption.…”

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

“…use of strong acids for demineralization) and technique of sterilization (Zhang et al 1997;Schwartz et al 1998Schwartz et al , 2007Herold et al 2002;Takikawa et al 2003;Bormann et al 2010;Katz 2010;Wei et al 2013). Thus, further in vitro and in vivo studies are required to assess the efficacy of DFDBA and FDBA and factors influencing their physical, chemical and bioactive properties.…”