The Effects of Varying Combinations of Dietary Selenium, Vitamin E, and Zinc Supplements on Antioxidant Enzyme Activity, and Developmental and Histological Traits in Testicular Tissues of 1-Year-Old Native Turkish Ganders

Abstract

The aim of this study was to determine the effects of varying combinations of dietary selenium (Se), vitamin E (Vit E), and zinc (Zn) supplements on antioxidant enzyme activity, and developmental and histological traits in testicular tissues of 1-year-old native Turkish ganders. A total of 48 animals were used and randomly assigned to 8 treatment groups (control, Se, Vit E, Zn, Se + Vit E, Se + Zn, Vit E + Zn, and Se + Vit E + Zn), with 6 birds in each group. In addition to the control (basic) diet, specific levels of supplements (0.3 mg/kg Se, 100 mg/kg Vit E, and 100 mg/kg Zn) were added to the diet of each treatment group. Antioxidative enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase activities, and malondialdehyde level) were more advantageous in the testicular tissue of ganders fed with Se + Vit E + Zn. Malondialdehyde (MDA), which is an important indicator of lipid peroxidation, was not significantly affected by the dietary treatments. However, it was negatively correlated with the seminiferous tubule area (−0.34) and diameter (−0.35). Compared to the control, the highest seminiferous tubule area and germinative epithelial thickness were determined as being fed with Se + Vit E + Zn. The lowest seminiferous tubule diameter was determined in the control and Zn groups, while the highest was in the group fed with Se + Vit E + Zn and Se + Vit E. This study showed that the simultaneous supplementation of Se + Vit and E + Zn into the diet of native Turkish ganders had positive effects on the testicular tissue, by reducing oxidative damage and improving histological parameters without affecting their physiological status.

The Effects of Varying Combinations of Dietary Selenium, Vitamin E, and Zinc Supplements on Semen Characteristics and Antioxidant Enzyme Activity of Spermatozoa in 1-Year-Old Native Turkish Ganders

“…Compared to the control group, the combined supplementation of Se + Vit E + Zn to diet, rather than using each separately, had a synergistic effect on sperm quality characteristics and increased semen volume, sperm concentration, sperm motility, and SQF values by 40%, 15.5%, 16.2%, and 79%, respectively. This improvement in semen quality is quite consistent with our results in Baş et al [26], the first study in which we revealed the histological parameters and oxidative enzyme activity in the testicular tissue of 1-year-old native Turkish ganders.…”

“…The antioxidant enzyme activity of the testicular tissue found in Baş et al's [26] study and the enzyme activity levels of the sperm cells determined in this study were quite parallel. Thus, by reducing oxidative damage in testis tissues with antioxidative feeding (Se, Vit E, Zn) in ganders, seminiferous tubule area and diameter and germinal cell layer thickness increased and the relative area of interstitial tissue decreased, which promoted the increase of sperm production and quality, which was also consistent with Malaniuk and Lukaszewicz [40], Edens and Sefton [41] and Baş et al [26]. The variation of sperm quality and antioxidant enzyme activity parameter values obtained according to treatments in this study is also consistent with previous studies [9,29].…”

“…This study consisted of 8 dietary treatments (Control, Se, Vit E, Zn, Se + Vit E, Se + Zn, Vit E + Zn, Se + Vit E + Zn), each with 6 ganders. Ganders were randomly assigned to each treatment, and their average initial body weight was 3976.5 g. In this study, while the semen characteristics of ganders were examined, testicular histology was investigated in our previous study [26], and these two studies were complementary.…”

Dietary composition influences sperm quality and testis damage via endoplasmic reticulum stress in lambs

“…Bas et al. ( 2023 ) reported that vitamin E, Zn and Se minerals added to the feed of Turkish geese at different ratios positively improved SOD, catalase, GSH peroxidase and GSH‐ S ‐transferase activities in testicular tissues but had no effect on MDA levels. Jiang et al.…”

Evaluation of Salvia hispanica as a Therapeutic Agent against Sodium Arsenic-Induced Testicular Toxicity in a Male Rats Model

“…The levels of glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) were measured [40].…”