The Effects of Ultra-high Dose Rates on Survival and Sublethal Repair in Chinese-hamster Cells

Schulz, Robert; Nath, Ravinder; Testa, Joseph R.

doi:10.1080/09553007714551521

Cited by 11 publications

(7 citation statements)

References 9 publications

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“…If the calculated D o values ( Table 1) and the actual cell survival for doses greater than 4 Gy are used as criteria, both pulsed electrons and pulsed X rays (bremsstrahlung) from the LINAC a more efficient in killing mammalian cells than continuous exposure to Cesium v-rays at moderate and lower dose rates. This finding appears to be in contrast to studies by other investigators, who have compared mammalian cell survival after exposure to ultrahigh dose rate, pulsed electrons (30 MeV) versus exposure to 280 kVp X rays; they reported that X rays were more effective for cell lethality (3,4). In tht latter expeyimencs (4), the average dose rate per electron pulse was 5 x 10…”

contrasting

confidence: 60%

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Survival of Chinese Hamster Ovary Cells Following Ultrahigh Dose Rate Electron and Bremsstrahlung Radiation

Holahan¹,

Meltz²

1990

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Ila. REPORT SEC'.jRITY CLASS;FICATON 16. RESTRiCTIVE MARKINGS Unclassified 2a. SECURITY CLASSIFICATION AUTHORITY 3 DISTRIBUTION!AVAILABILITY OF REPORT j Approved for public release; distribution , 2b DECLASSIFiCATIoNDOWNGROING SC.-DUL-is unl imited. 4. PERFOR'ING ORGANZATjON aC.. NUM,8L,(S) 5 MONITORINQ ORGANIZATIV.i :EPcORT NUM .S-'SI USAFSAM-TR-90-4 6,3 NAME CF PERFORMING ORGANZA'!0% 15 OFFICE SYMBOL 7a NAME OF MONITORING ORGAN:ZA~iON

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contrasting

confidence: 60%

“…A variety of studies have been conducted using pulsed electron radiation in nanosecond (ns) to microsecond (As) pulses (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

Survival of Chinese Hamster Ovary Cells Following Ultrahigh Dose Rate Electron and Bremsstrahlung Radiation

Holahan¹,

Meltz²

1990

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“…Further studied failed to clarify the influence of UHDR and pulsed irradiation modality on cellular response. Schulz et al [22] found a pulsed 30-MeV electron beam at 0.5 -5 10 8 Gy/s to be less effective at cell killing compared to 250 kV X-rays, but they could not relate their results to oxygen consumption. Purdie et al [23] found opposite results using similar experimental conditions.…”

Section: Figurementioning

confidence: 99%

The radiobiology of laser-driven particle beams: focus on sub-lethal responses of normal human cells

et al. 2017

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A: Accelerated proton beams have become increasingly common for treating cancer. The need for cost and size reduction of particle accelerating machines has led to the pioneering investigation of optical ion acceleration techniques based on laser-plasma interactions as a possible alternative. Laser-matter interaction can produce extremely pulsed particle bursts of ultra-high dose rates (≥ 10 9 Gy/s), largely exceeding those currently used in conventional proton therapy. Since biological effects of ionizing radiation are strongly affected by the spatio-temporal distribution of DNA-damaging events, the unprecedented physical features of such beams may modify cellular and tissue radiosensitivity to unexplored extents. Hence, clinical applications of laser-generated particles need thorough assessment of their radiobiological effectiveness. To date, the majority of studies have either used rodent cell lines or have focussed on cancer cell killing being local tumour control the main objective of radiotherapy. Conversely, very little data exist on sub-lethal cellular effects, of relevance to normal tissue integrity and secondary cancers, such as premature cellular senescence. Here, we discuss ultra-high dose rate radiobiology and present preliminary 1Corresponding author.

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“…Therefore, the biological effectiveness of laser-accelerated particles has to be characterized in appropriate experiments. Previous studies have been performed with ultrashort pulsed electron [17][18][19] and soft x-ray [20][21][22] beams delivered with maximum pulse dose rates of 10 9 Gy/ s ͑Ref. 19͒ for electrons and 10 13 Gy/ s ͑Ref.…”

Section: Introductionmentioning

confidence: 99%

“…20͒ for photons, respectively. In all of these studies the cellular survival was investigated, revealing either no influence 18,19,21 or a reduction 17,20,22 in the cytotoxicity by means of the ultrahigh dose rate.…”

Section: Introductionmentioning

confidence: 99%

Establishment of technical prerequisites for cell irradiation experiments with laser-accelerated electrons

et al. 2010

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Basic technical prerequisites for future cell irradiation experiments with ultrashort pulsed laser-accelerated electrons were established at the JETI laser system. The implemented online control system is suitable to compensate beam intensity fluctuations and the achieved accuracy of dose delivery to the cells is sufficient for radiobiological cell experiments. Hence, systematic in vitro cell irradiation experiments can be performed, being the first step toward clinical application of laser-accelerated particles. Further steps, including the transfer of the established methods to experiments on higher biological systems or to other laser-based particle accelerators, will be prepared.

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The Effects of Ultra-high Dose Rates on Survival and Sublethal Repair in Chinese-hamster Cells

Cited by 11 publications

References 9 publications

Survival of Chinese Hamster Ovary Cells Following Ultrahigh Dose Rate Electron and Bremsstrahlung Radiation

Survival of Chinese Hamster Ovary Cells Following Ultrahigh Dose Rate Electron and Bremsstrahlung Radiation

The radiobiology of laser-driven particle beams: focus on sub-lethal responses of normal human cells

Establishment of technical prerequisites for cell irradiation experiments with laser-accelerated electrons

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