The effects of transformation and ZnT-1 silencing on zinc homeostasis in cultured cells

Sankavaram, Kavitha; Freake, Hedley C.

doi:10.1016/j.jnutbio.2011.03.006

Cited by 12 publications

(6 citation statements)

References 31 publications

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“…Because MT has a similar response, ZNT1 and MT may cooperatively manage cellular responses against zinc toxicity (13). To perform this func-tion efficiently, ZNT1 has been speculated to accumulate on the cell surface (33); however, this idea has not been proven experimentally. We show that ZNT1 protein accumulation on the A, Dox-dependent expression of WT ZNT1 and ZNT1 N299A mutant in MDCK FLp-In T-Rex (MDCK) cells.…”

Section: Zinc-responsive Znt1 Expressionmentioning

confidence: 99%

Zinc transporter 1 (ZNT1) expression on the cell surface is elaborately controlled by cellular zinc levels

Nishito¹,

Kambe

2019

Journal of Biological Chemistry

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Section: Zinc-responsive Znt1 Expressionmentioning

confidence: 99%

Zinc transporter 1 (ZNT1) expression on the cell surface is elaborately controlled by cellular zinc levels

Nishito¹,

Kambe

2019

Journal of Biological Chemistry

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“…This demonstrates the importance of intracellular zinc for rapidly dividing cancer cells. Experiments using shRNA against Znt-1, the only currently recognized plasma membrane zinc efflux transporter, have more recently demonstrated the requirement of Znt-1 for these effects [37]. …”

Section: The Role Of Zinc In Cancermentioning

confidence: 99%

Zinc and Cancer: Implications for LIV-1 in Breast Cancer

Grattan

Freake

2012

Nutrients

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Zinc is a trace mineral which is vital for the functioning of numerous cellular processes, is critical for growth, and may play an important role in cancer etiology and outcome. The intracellular levels of this mineral are regulated through the coordinated expression of zinc transporters, which modulate both zinc influx as well as efflux. LIV-1 (ZIP6) was first described in 1988 as an estrogen regulated gene with later work suggesting a role for this transporter in cancer growth and metastasis. Despite evidence of its potential utility as a target gene for cancer prognosis and treatment, LIV-1 has received relatively little attention, with only three prior reviews being published on this topic. Herein, the physiological effects of zinc are reviewed in light of this mineral’s role in cancer growth with specific attention being given to LIV-1 and the potential importance of this transporter to breast cancer etiology.

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“…Slc30a1/Znt1 is well-known as a crucial regulator of zinc absorption and transport [ 64 ]. Znt1 reduces glial and neuronal zinc levels, protecting these cells from zinc toxicity and reducing their deaths [ 65 , 66 ]. The proteomics data herein also confirmed that Znt1 levels are significantly increased in VD mice treated with JTD, further confirming the neuroprotective effects of JTD.…”

Section: Discussionmentioning

confidence: 99%

Integrated Network Pharmacology and Proteomic Analyses of Targets and Mechanisms of Jianpi Tianjing Decoction in Treating Vascular Dementia

Liu

Gong

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Background. Vascular dementia (VD), associated with cerebrovascular injury, is characterized by severe cognitive impairment. Jianpi Tianjing Decoction (JTD) has been widely used to treat VD. However, its molecular targets and mechanisms of action in this treatment remain unclear. This study integrated network pharmacology and proteomics to identify targets and mechanisms of JTD in the treatment of VD and to provide new insights and goals for clinical treatments. Methods. Systematic network pharmacology was used to identify active chemical compositions, potential targets, and mechanisms of JTD in VD treatment. Then, a mouse model of VD was induced via transient bilateral common carotid artery occlusion to verify the identified targets and mechanisms of JTD against VD using 4D label-free quantitative proteomics. Results. By screening active chemical compositions and potential targets in relevant databases, 187 active chemical compositions and 416 disease-related compound targets were identified. In vivo experiments showed that JTD improved learning and memory in mice. Proteomics also identified 112 differentially expressed proteins in the model and sham groups and the JTD and model groups. Integrating the network pharmacology and proteomics results revealed that JTD may regulate expressions of cytochrome c oxidase subunit 7C, metabotropic glutamate receptor 2, Slc30a1 zinc transporter 1, and apolipoprotein A-IV in VD mice and that their mechanisms involve biological processes like oxidative phosphorylation, regulation of neuron death, glutamate secretion, cellular ion homeostasis, and lipoprotein metabolism. Conclusions. JTD may suppress VD development via multiple components, targets, and pathways. It may thus serve as a complementary treatment option for patients with VD.

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The effects of transformation and ZnT-1 silencing on zinc homeostasis in cultured cells

Cited by 12 publications

References 31 publications

Zinc transporter 1 (ZNT1) expression on the cell surface is elaborately controlled by cellular zinc levels

Zinc transporter 1 (ZNT1) expression on the cell surface is elaborately controlled by cellular zinc levels

Zinc and Cancer: Implications for LIV-1 in Breast Cancer

Integrated Network Pharmacology and Proteomic Analyses of Targets and Mechanisms of Jianpi Tianjing Decoction in Treating Vascular Dementia

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