THE EFFECTS OF TIME AND INDOMETHACIN ON CONTRACTILE RESPONSES OF THE GUINEA‐PIG GALL BLADDER <i>in vitro</i>

Doggrell, Sheila A; Scott, G. W.

doi:10.1111/j.1476-5381.1980.tb10955.x

Cited by 12 publications

(9 citation statements)

References 10 publications

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“…Thus it seems likely that in the absence of antagonists, (-)noradrenaline and (--adrenaline act at both a-and (-adrenoceptors, the overall contractile response observed being the net effect of contraction and relaxation. The responses to continuous field stimulation of gall bladder strips were abolished and reduced, respectively, by tetrodotoxin and atropine (Doggrell & Scott, 1979). This confirms the excitatory cholinergic innervation to the guinea-pig gall bladder (Davison & Fosel, 1975 …”

Section: Effects Of Phentolamine and Propranololsupporting

confidence: 70%

“…The contractile responses to (-)-noradrenaline, 10-5 M, were converted to relaxations in the presence of 10-6 M phentolamine (Figure 1 (Doggrell & Scott, 1979) are observed. The contractile responses to field stimulation (in the absence or presence of atropine), to (-)-noradrenaline, to acetylcholine and to ATP increase in magnitude with time.…”

Section: Effects Of Phentolamine and Propranololmentioning

confidence: 99%

“…have been demonstrated clearly. Strips of guinea-pig gall bladder contract in response to (--noradrenaline and relax to (-)-isoprenaline (Doggrell & Scott, 1979). However, the adrenoceptors in the guinea-pig gall bladder have not been characterized.…”

Section: Introductionmentioning

confidence: 99%

“…The magnitude of the relaxant responses to (--isoprenaline initially decreases and ** then increases with time. Doggrell & Scott (1979) have suggested that these time-dependent changes are prostaglandin-mediated and emphasized the necessity of carefully controlling experiments using this prep-2 3…”

Section: Effects Of Phentolamine and Propranololmentioning

confidence: 99%

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The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

Doggrell

Scott

1980

British J Pharmacology

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I Guinea-pig gall bladder strips were contracted by (--noradrenaline, 10-5 M, and by field stimulation at 5 Hz (in the absence or presence of 10-6 M atropine) and relaxed to 10-s M (--isoprenaline. (-)-Adrenaline, 10-5 M, predominantly contracted, but sometimes relaxed, this preparation. 2 In the presence of 10-6 M phentolamine, contractions to (--noradrenaline and to (-)-adrenaline were reversed to relaxations. The relaxations produced by (-)-isoprenaline were unaltered. In the presence of 10'6 M propranolol, contractions to (-)-noradrenaline increased in magnitude, relaxations to (-)-adrenaline were reversed to contractions, and relaxations to (-)-isoprenaline were abolished. These results demonstrate the presence of postsynaptic ax-adrenoceptors which mediate contractions, and postsynaptic ,B-adrenoceptors which initiate relaxations, in the guinea-pig gall bladder. 3 The contractile responses to continuous field stimulation for 5 min at 5 Hz in Krebs solution alone were reduced in magnitude by propranolol, 10-6 M. In the presence of 10-6 M atropine (added to eliminate the cholinergic component of the response), propranolol, 1lo' M, had no effect on responses to stimulation at 5 Hz. Thus propranolol reduced the response to cholinergic stimulation in this tissue; the basis of this effect is unclear. In the absence or presence or atropine (10-6 M), the responses to 5 Hz were smaller in magnitude in the presence than absence of phentolamine, 10-6 M. This suggests that the responses to field stimulation of the guinea-pig gall bladder may, in part, be due to the release of endogenous noradrenaline which acts at postsynaptic a-adrenoceptors.

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Section: Effects Of Phentolamine and Propranololsupporting

confidence: 70%

Section: Effects Of Phentolamine and Propranololmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Effects Of Phentolamine and Propranololmentioning

confidence: 99%

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The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

Doggrell

Scott

1980

British J Pharmacology

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“…There is ample evidence in the basic science literature, demonstrating the effects of COX-inhibitors on micturition and/or bladder contractile activity in rats [64], rabbits [65], guinea-pigs [66] and cats [67]. However, clinical studies demonstrating efficacy of these agents in the treatment of voiding dysfunction are limited.…”

Section: Discussionmentioning

confidence: 99%

Potential for control of detrusor smooth muscle spontaneous rhythmic contraction by cyclooxygenase products released by interstitial cells of Cajal

Collins

Klausner

Herrick

et al. 2009

J Cellular Molecular Medi

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Interstitial cells of Cajal (ICCs) have been identified as pacemaker cells in the upper urinary tract and urethra, but the role of ICCs in the bladder remains to be determined. We tested the hypotheses that ICCs express cyclooxygenase (COX), and that COX products (prostaglandins), are the cause of spontaneous rhythmic contraction (SRC) of isolated strips of rabbit bladder free of urothelium. SRC was abolished by 10 μM indomethacin and ibuprofen (non-selective COX inhibitors). SRC was concentration-dependently inhibited by selective COX-1 (SC-560 and FR-122047) and COX-2 inhibitors (NS-398 and LM-1685), and by SC-51089, a selective antagonist for the PGE-2 receptor (EP) and ICI-192,605 and SQ-29,548, selective antagonists for thromboxane receptors (TP). The partial agonist/antagonist of the PGF-2α receptor (FP), AL-8810, inhibited SRC by ∼50%. Maximum inhibition was ∼90% by SC-51089, ∼80–85% by the COX inhibitors and ∼70% by TP receptor antagonists. In the presence of ibuprofen to abolish SRC, PGE-2, sulprostone, misoprostol, PGF-2α and U-46619 (thromboxane mimetic) caused rhythmic contractions that mimicked SRC. Fluorescence immunohistochemistry coupled with confocal laser scanning microscopy revealed that c-Kit and vimentin co-localized to interstitial cells surrounding detrusor smooth muscle bundles, indicating the presence of extensive ICCs in rabbit bladder. Co-localization of COX-1 and vimentin, and COX-2 and vimentin by ICCs supports the hypothesis that ICCs were the predominant cell type in rabbit bladder expressing both COX isoforms. These data together suggest that ICCs appear to be an important source of prostaglandins that likely play a role in regulation of SRC. Additional studies on prostaglandin-dependent SRC may generate opportunities for the application of novel treatments for disorders leading to overactive bladder.

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Time-dependent augmentation of the contractile responses to adrenaline and noradrenaline of the guinea-pig esophageal muscularis mucosae in vitro

Uchida

Kamikawa

Shimo

1983

Naunyn-Schmiedeberg's Arch. Pharmacol.

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THE EFFECTS OF TIME AND INDOMETHACIN ON CONTRACTILE RESPONSES OF THE GUINEA‐PIG GALL BLADDER in vitro

Cited by 12 publications

References 10 publications

The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

Potential for control of detrusor smooth muscle spontaneous rhythmic contraction by cyclooxygenase products released by interstitial cells of Cajal

Time-dependent augmentation of the contractile responses to adrenaline and noradrenaline of the guinea-pig esophageal muscularis mucosae in vitro

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