The effects of the initial treatment phase and of adjunctive low-dose doxycycline therapy on clinical parameters and MMP-8, MMP-9, and TIMP-1 levels in the saliva and peripheral blood of patients with chronic periodontitis

Górska, Renata; Nędzi-Góra, Małgorzata

doi:10.1007/s00005-006-0047-6

Cited by 41 publications

(47 citation statements)

References 39 publications

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“…A presença da placa bacteriana e seus metabólitos estimulam as células do periodonto a produzirem citocinas pró-inflamatórias, tais como: TGF-β, IL-4, -10 e -12 (Page 1991) que estimulam a liberação de outras citocinas (IL-1, IL-6, TNF-α e PGE 2 ) e enzimas neste tecido (Pozo, Valenzuela et al 2005). Assim, fibroblastos, células do infiltrado inflamatório e outras células do periodonto passam a produzir MMPs que degradam a ECM do tecido conjuntivo periodontal, levando a formação dos bolsos periodontais e conseqüente perda de massa óssea (Gorska and Nedzi-Gora 2006).…”

Section: Doença Periodontalunclassified

“…Também é possível observar uma alteração da liberação dos TIMPs, principalmente o TIMP-1, que é produzido pelas células estacionárias do periodonto, macrófagos e monócitos (Gorska and Nedzi-Gora 2006).…”

Section: Doença Periodontalunclassified

“…Enquanto alguns autores não encontraram diminuição da MMP-9 após o tratamento não cirúrgico (Sorsa, Ding et al 1994;Gorska and Nedzi-Gora 2006), trabalhos mais recentes tem observado um aumento de MMP-9 e TIMP-1 dosados por ELISA em pacientes com DP (Isaza-Guzman, Arias-Osorio et al 2011). Ainda, outros artigos mostram a diminuição da MMP-9 apenas após 12 meses o tratamento de periodontite moderada (Kinney, Morelli et al 2011).…”

Section: Steunclassified

“…Os pacientes com DP apresentam maiores concentrações de MMP-8 na saliva em relação aos controles, e ocorre a diminuição dessa colagenase após o tratamento da doença em questão (Gorska and Nedzi-Gora 2006;Buduneli, Mantyla et al 2010). …”

Section: Steunclassified

“…Também tem sido mostrado que a MMP-8 analisada pela técnica de ELISA diminui após o tratamento periodontal não cirúrgico, assim como no tratamento com doxyciclina (Sorsa, Ding et al 1994;Gorska and Nedzi-Gora 2006;Sexton, Lin et al 2011). …”

Section: Steunclassified

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Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Meschiari¹

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Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases known to cleave components of the connective tissue in physiological and pathological processes. The regulation of MMP activities is done by the tissue inhibitors of metalloproteinases (TIMPs).Periodontal disease (PD) is a chronic inflammation with excessive activity of MMPs, which degrade the tooth supporting tissues.The saliva components are derived from the local blood supply, and this fluid may therefore reflect the plasma. So, the objectives of this study were: 1) to compare the levels of MMPs, TIMPs and MPO in stimulated whole saliva (SWS) and plasma of PD patients before (PB) and 3 months after (PT) the non-surgical periodontal treatment, and in healthy volunteers at baseline (CB) and 3 months after baseline (CT), and 2) to evaluate the correlations between the results found.Measurements of MMP-8, MMP-9, TIMP-1 and TIMP-2 were performed by ELISA. Gelatinolitic activity of MMP-9 forms were determined by zymography, and the MPO activity was determined by colorimetric assay.There was lower gelatinolitic activity, and lower concentrations of MMP-8 and TIMP-2 in STE on PT group compared with PB group (p <0.05). MPO activity was higher in PB compared to CB (p <0.05).Statistically significant moderate correlations were observed in all associations between MMP-9 performed by ELISA in plasma and gelatinolitic activity bands of STE: MMP-9 molecular form of 92 kDa (r = 0,37, p = 0,0017), MMP-9 molecular form of 130 kDa (r = 0,35, p = 0,003), the sum of its gelatinase activity (130 +92 kDa) (r = 0,43, p = 0,0002), gelatinase of 180 kDa (r = 0,35, p = 0,003), and the sum of total gelatinase activity (180+130+92 kDa) (r = 0,37, p = 0,002). Circulating MMP-8 levels correlate with salivary gelatinase activity bands of 92 kDa (r = 0,30, p = 0,01) and the sum of gelatinase activity (130 +92 kDa) (r = 0,24, p = 0,04). In addition, a weak correlation between gelatinase activity of plasmatic 92 kDa MMP-9 and gelatinase of 180 kDa in SWS (r= 0,26 p = 0,03) was found, and a moderate correlation between plasmatic TIMP-2 and TIMP-2 of SWS (r = 0,32, p = 0,004).The results show that the gelatinase activity of SWS may reflect the concentrations of systemic inflammatory markers such as MMP-8 and MMP-9, also the concentration of TIMP-2 in the SWS may reflect the circulating concentration of TIMP-2. The evaluation of these results suggests that there is an attenuation of some inflammatory markers analyzed in the SWS and in plasma after treatment of PD. Furthermore, there are correlations between salivary and plasma levels in some of these markers. Because saliva sampling is less invasive, more studies about the correlations between these markers in these two fluids are necessary. Key words: saliva, plasma, matrix metalloproteinases, periodontal disease. LISTA DE ABREVIATURAS, SIGLAS E SÍMBOLOS BSA -

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Section: Doença Periodontalunclassified

Section: Steunclassified

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Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Meschiari¹

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Salivary MMP‐13 gender differences in periodontitis: A cross‐sectional study from Sweden

Virtanen

Yakob

Tervahartiala

et al. 2017

Clinical & Exp Dental Res

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We investigated serum and saliva concentrations of matrix metalloproteinases, MMP‐8, MMP‐9, and MMP‐13, and their tissue inhibitor TIMP‐1, in a group of patients with and without periodontitis from Sweden. The hypothesis was that these biomarkers are higher in the periodontitis patients. Ninety patients participated in this cross‐sectional study. Fifty‐one patients had periodontitis whereas 39 were periodontally healthy. Saliva and serum samples were analyzed with immunofluorometric, enzyme‐linked immunosorbent assay and western blot. Results were statistically analyzed with independent t test, Mann–Whitney U test, Bonferroni corrections, and regression analyses. MMP‐13 was not detected in serum, but in saliva, higher values were found among the periodontally healthy compared with periodontitis subjects (0.32 ± 0.26 vs. 0.21 ± 0.23 ng/ml, p < .05). Female gender and clinical attachment loss were the explanatory factors for higher salivary MMP‐13 values with odds ratio 3.08 (95% confidence interval [1.17, 8.11]) and 3.57 (95% confidence interval [1.08, 11.82]), respectively. No statistically significant differences between groups were found in serum and saliva values of MMP‐8, MMP‐9, and TIMP‐1. Contrary to our hypothesis, no statistically significant differences between patients with and without periodontitis were seen in MMP‐8, MMP‐9, and TIMP‐1 values. However, higher MMP‐13 concentrations in saliva were associated with female gender and higher clinical attachment loss. Metabolism of MMP‐13 may thus have some gender implications in periodontitis.

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Particular Aspects of Periodontal Disease Pathogenesis

Dumitrescu¹,

Tanaka²

2010

Etiology and Pathogenesis of Periodontal Disease

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The effects of the initial treatment phase and of adjunctive low-dose doxycycline therapy on clinical parameters and MMP-8, MMP-9, and TIMP-1 levels in the saliva and peripheral blood of patients with chronic periodontitis

Abstract: The study confirmed the modulating effect of doxycycline on the host response in chronic periodontitis.

Cited by 41 publications

References 39 publications

Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Salivary MMP‐13 gender differences in periodontitis: A cross‐sectional study from Sweden

Particular Aspects of Periodontal Disease Pathogenesis

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