The Effects of the Ergosteroid 7-Oxo-dehydroepiandrosterone on Mitochondrial Membrane Potential: Possible Relationship to Thermogenesis

Bobyleva, Valentina; Bellei, Monica; Kneer, Nancy; Lardy, Henry A.

doi:10.1006/abbi.1997.9955

Cited by 26 publications

(14 citation statements)

References 36 publications

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“…It is also shown that succinate and CSA were unable to restore Ψ to a high level. Bobyleva et al [25] have shown that DHEA induces mitochondrial proton leakage, Thus, a question arises about the requirement for Ca 2+ to achieve the effect of DHEA on membrane permeability. To answer such a question DHEA was added previously to Ca 2+ addition.…”

Section: Resultsmentioning

confidence: 99%

Dehydroepiandrosterone as an inducer of mitochondrial permeability transition

Correa

Garcı́a

García

et al. 2003

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Resultsmentioning

confidence: 99%

Dehydroepiandrosterone as an inducer of mitochondrial permeability transition

Correa

Garcı́a

García

et al. 2003

The Journal of Steroid Biochemistry and Molecular Biology

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“…It should be considered that although these polyclonal antibodies were raised against a highly purified 32 kDa PUMP, the presence of a low quantity of contaminating ATP/ADP antiporter cannot be totally excluded. In this regard, we must recall that the ATP/ADP carrier, which may also mediate FA uncoupling [5] and be induced by cold acclimation in animals [20–22], could be contributing to the increase in the protein bands observed in Fig. 3 and in the mitochondrial uncoupling observed in Figs.…”

Section: Resultsmentioning

confidence: 99%

Low temperature and aging‐promoted expression of PUMP in potato tuber mitochondria

et al. 1999

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In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28³C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4³C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential (v v8 8). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on v v8 8, the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.z 1999 Federation of European Biochemical Societies.

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“…DHEA may exert its pleiotropic effects following its conversion into several biologically-active metabolites, including 7α-hydroxy-DHEA (7α-OH-DHEA), 7β-hydroxy-DHEA (7β-OH-DHEA) and 7-oxo-DHEA [11,20]. For example, 7-oxo-DHEA, as well as 7α-and 7β-OH-DHEA, have been proposed to have biological actions in rodent and men [21][22][23][24] with higher effectiveness than the parent sterol DHEA [25,26].…”

Section: Discussionmentioning

confidence: 99%

Interactions between dehydroepiandrosterone and glucocorticoid metabolism in pig kidney: Nuclear and microsomal 11β-hydroxysteroid dehydrogenases

Robinzon

Prough

2005

Archives of Biochemistry and Biophysics

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The 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) activates glucocorticoids (GC) by reversibly converting 11-keto-GC to 11-hydroxy-GC, while 11βHSD2 and 11βHSD3 only catalyzes the reverse reaction. Recently, rat and human 11βHSDs were shown to interconvert 7α-and 7β-hydroxy-dehydroepiandrosterone (7α-or 7β-OH-DHEA) with 7-oxo-DHEA. We report that pig kidney microsomes (PKMc) and nuclei (PKN) oxidize 7α-OH-DHEA to 7-oxo-DHEA at higher rates with NAD + , than with NADP + . Corticosterone (CS), dehydrocoticosterone (DHC), 11α-and 11β-hydroxyprogesterone, and carbenoxolone completely inhibited these reactions, while 7-oxo-DHEA only inhibited the NAD + -dependent reaction. Conversely, CS oxidation was not inhibited by 7α-OH-DHEA or 7-oxo-DHEA. PKMc and PKN did not convert 7-oxo-DHEA to 7-OH-DHEA with either NADPH or NADH. Finally, PKN contained a high affinity, NADPH-dependent 11βHSD that reduces DHC to CS. The GC effects on interconversion of DHEA metabolites may have clinical significance, since DHEA and its 7-oxidized derivatives have been proposed for treatment of human autoimmune and inflammatory disorders.

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The Effects of the Ergosteroid 7-Oxo-dehydroepiandrosterone on Mitochondrial Membrane Potential: Possible Relationship to Thermogenesis

Cited by 26 publications

References 36 publications

Dehydroepiandrosterone as an inducer of mitochondrial permeability transition

Dehydroepiandrosterone as an inducer of mitochondrial permeability transition

Low temperature and aging‐promoted expression of PUMP in potato tuber mitochondria

Interactions between dehydroepiandrosterone and glucocorticoid metabolism in pig kidney: Nuclear and microsomal 11β-hydroxysteroid dehydrogenases

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