The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Jandera, Pavel; Staňková, Magda

doi:10.1007/s10337-015-2848-4

Cited by 7 publications

(2 citation statements)

References 19 publications

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“…different instrumentation) may skew resulting efficiency, especially on instrumentation where extra‐column contribution is not optimized. When extra‐column volumes are minimized, column efficiency improves significantly .…”

Section: Discussionmentioning

confidence: 99%

Current trends in the development of porous polymer monoliths for the separation of small molecules

Urban

2015

J of Separation Science

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Since their introduction, the main application area of porous polymer monoliths has been in the fast gradient separation of synthetic and natural polymers. On the other hand, it has proven to be difficult to prepare polymer monoliths providing column efficiency comparable with particulate and monolithic silica-based stationary phases. During this decade, several experimental approaches were performed that aimed to improve this property of polymer monoliths. These protocols include variation in a polymerization time and preparation of monolithic stationary phases at limited conversion of the polymerization reaction, application of novel, highly ordered, nanomaterials, and/or hypercross-linking surface modification controlling the cross-link density of prepared monoliths. By using some of these approaches, monolithic stationary phases with column efficiency reaching 200,000 plates/m for low-molecular-weight compounds have been prepared. This review deals with preparation of polymer monoliths for the separation of small molecules and summarizes recent development in this field. At first, it focuses on monolithic columns morphology and repeatability of their preparation. Then, recent results in individual experimental protocols are discussed. Finally, possible future steps leading to the preparation of more efficient monolithic stationary phases are outlined.

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Section: Discussionmentioning

confidence: 99%

Current trends in the development of porous polymer monoliths for the separation of small molecules

Urban

2015

J of Separation Science

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“…Akan tetapi, untuk senyawa yang memiliki sifat kepolaran yang sangat tinggi, pemisahan dengan menggunakan metode RPLC tidak cukup efisien karena senyawa akan tertahan lebih lama di dalam kolom pemisah yang memilik sifat hidrofobik yang tinggi. Sehingga pada kondisi tersebut, pemisahan dengan menggunakan metode yang dikenal dengan Hidrophilic Interaction Liquid Chromatography (HILIC) menjadi pilihan [6][7][8]. Meskipun demikian, pemisahan sampel yang lebih kompleks dengan melibatkan kombinasi senyawa hidrofobik dan hidrofilik, dan hanya menggunakan satu kolom pemisah menjadi tantangan pada analisa dengan HPLC metode tunggal.…”

Section: Pendahuluanunclassified

Pembuatan Kolom Monolit Mixed-Mode untuk Pemisahan Fenol dalam Kromatografi Cair Sistem Kapiler

Rahayu

Fajri

Lim

et al. 2018

CHEMICA: Jurnal Teknik Kimia

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Dual Retention Mechanism in Two-Dimensional LC Separations of Barbiturates, Sulfonamides, Nucleic Bases and Nucleosides on Polymethacrylate Zwitterionic Monolithic Micro-Columns

Staňková

Jandera

2016

Chromatographia

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The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Cited by 7 publications

References 19 publications

Current trends in the development of porous polymer monoliths for the separation of small molecules

Current trends in the development of porous polymer monoliths for the separation of small molecules

Pembuatan Kolom Monolit Mixed-Mode untuk Pemisahan Fenol dalam Kromatografi Cair Sistem Kapiler

Dual Retention Mechanism in Two-Dimensional LC Separations of Barbiturates, Sulfonamides, Nucleic Bases and Nucleosides on Polymethacrylate Zwitterionic Monolithic Micro-Columns

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