The effects of the amino acid canavanine on Pythium

Barron, James A.; Weaks, T. E.

doi:10.1016/0048-4059(77)90073-x

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…In addition, Barron and Weaks, (1977) [ 46 ] reported a canavanine-mediated enhancement of growth in several isolates of Pythium spp. The underlying mechanism of action appears to be the same as reported for the toxic effect of canavanine in insects, i.e., it is falsely incorporated into the microbial proteins [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli

et al. 2023

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In this study, the allelopathic properties of Medicago sativa L. (alfalfa) seedling exudates on the germination of seeds of various species were investigated. The compounds responsible for the allelopathic effects of alfalfa were identified and characterized by employing liquid chromatography ion mobility high-resolution mass spectrometry. Crude exudates inhibited the germination of seeds of all various plant species tested. Overall, nine compounds in alfalfa were identified and quantified. The most predominant compounds were a hyperoside representing a flavonoid glucoside, the non-proteinogenic amino acid canavanine, and two dipeptides, identified as H-Glu-Tyr-OH and H-Phe-Glu-OH. The latter corresponds to the first finding that dipeptides are exuded from alfalfa seedlings. In addition, the antibacterial and antibiofilm activities of alfalfa exudate and its identified compounds were elucidated. Both hyperoside and canavanine revealed the best antibacterial activity with minimum inhibitory concentration (MIC) values that ranged from 8 to 32 and 32 to 256 µg/mL, respectively. Regarding the antibiofilm action, hyperoside and canavanine caused a decline in the percentage of E. coli isolates that possessed a strong and moderate biofilm-forming potential from 68.42% to 21.05% and 31.58%, respectively. Studies on their inhibiting effects exhibit that these major substances are predominantly responsible for the allelopathic and antimicrobial effects of the crude exudates.

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Section: Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli

et al. 2023

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“…The toxic effect of canavanine on phytophagous insects has been well-established (28), but the effect of canavanine on soil microorganisms has been largely ignored except for studies in which inhibition by canavanine in culture was examined (2,33,34). This is the first study of the effect of canavanine on microorganisms under soil conditions.…”

Section: Discussionmentioning

confidence: 99%

Effect of Canavanine from Alfalfa Seeds on the Population Biology of Bacillus cereus

Emmert

Milner

Lee

et al. 1998

Appl Environ Microbiol

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Bacillus cereus UW85 suppresses diseases of alfalfa seedlings, although alfalfa seed exudate inhibits the growth of UW85 in culture (J. L. Milner, S. J. Raffel, B. J. Lethbridge, and J. Handelsman, Appl. Microbiol. Biotechnol. 43:685–691, 1995). In this study, we determined the chemical basis for and biological role of the inhibitory activity. All of the alfalfa germ plasm tested included seeds that released inhibitory material. We purified the inhibitory material from one alfalfa cultivar and identified it as canavanine, which was present in the cultivar Iroquois seed exudate at a concentration of 2 mg/g of seeds. Multiple lines of evidence suggested that canavanine activity accounted for all of the inhibitory activity. Both canavanine and seed exudate inhibited the growth of UW85 on minimal medium; growth inhibition by either canavanine or seed exudate was prevented by arginine, histidine, or lysine; and canavanine and crude seed exudate had the same spectrum of activity against B. cereus, Bacillus thuringiensis, and Vibrio cholerae. The B. cereus UW85 populations surrounding canavanine-exuding seeds were up to 100-fold smaller than the populations surrounding non-canavanine-exuding seeds, but canavanine did not affect the growth of UW85 on seed surfaces. The spermosphere populations of canavanine-resistant mutants of UW85 were larger than the spermosphere populations of UW85, but the mutants and UW85 were similar in spermoplane colonization. These results indicate that canavanine exuded from alfalfa seeds affects the population biology of B. cereus.

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Raman Optical Activity

Barron

1979

Optical Activity and Chiral Discrimination

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The effects of the amino acid canavanine on Pythium

Cited by 3 publications

References 18 publications

Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli

Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli

Effect of Canavanine from Alfalfa Seeds on the Population Biology of Bacillus cereus

Raman Optical Activity

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