The Effects of Syzygium aromaticum extract Spread on the Allergic Contact Dermatitis induced by DNCB

Abstract

Objectives : The purpose of this study is to investigate effects of Syzygium aromaticum (SAE) spread on the allergic contact dermatitis caused by 2,4-dinitro-chlorobezene (DNCB). Methods : Forty-two mice were divided into six groups ; normal, negative control (DNCB-treated), positive control (DNCB + 1% pimecrolimus), experimental group Ⅰ, Ⅱ and Ⅲ. control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group Ⅰ(DNCB + 0.2% SAE), Ⅱ(DNCB + 1% SAE) and Ⅲ(DNCB + 5% SAE) were spread SAE and positive control was spread the 1% pimecrolimus. In this study, effect of SAE on clinical aspects on the skin, histopathological change, the blood level of IgE, cytokines, histamine were investigated. In addition, effect of SAE on spleen CD4