The effects of synthesized silver nanowires on the structure and esterase-like activity of human serum albumin and their impacts on human endometrial stem cells

Hekmat, Azadeh; Hatamie, Shadie; Saboury, Ali Akbar

doi:10.1080/24701556.2022.2034859

Cited by 4 publications

(11 citation statements)

References 64 publications

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“…As demonstrated in the insets of Figure 3e,f, the experimental Stern‐Volmer plot for HA and FA displayed a positive deviation. Consequently, the mechanism of binding of HA and FA to tyrosinase was initiated mainly through the formation of the nonfluorescence complex (static quenching) (Hekmat et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…By adding portions of PBS, the concentration of tyrosinase and the sample volume were maintained constant. For inner filter impact correction affected by the emission and excitation signals attenuation generated from the quencher absorption, Equation 1 was applied (Hekmat et al, 2021):

{F}_{corr}={F}_{obs}\cdotp {10}^{\left({Ab}_{ex}+{Ab}_{em}\right)/2},

where Ab em , Ab ex , F obs , and F corr are the mixture absorption at emission as well as excitation wavelengths, the observed fluorescence intensity, and the corrected fluorescence intensity, respectively (Pashah et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Section: The Intrinsic Fluorescence Measurementsmentioning

confidence: 99%

“…In this equation, F, F 0 K sv , and [Q] are the fluorescence intensity with a quencher (HA or FA), the fluorescence intensity without a quencher, the Stern-Volmer quenching constant, and the HA or FA concentrations, respectively. Applying Equation 5, the plots for of HA and FA to tyrosinase was initiated mainly through the formation of the nonfluorescence complex (static quenching) (Hekmat et al, 2021).…”

Section: Mechanism Of Fluorescence Quenchingmentioning

confidence: 99%

“…to molecular interactions in the formation of HA or FA complex with tyrosinase (Hekmat et al, 2022). K A is an indication of the presence of binding sites in a biomacromolecule whose occupation relies on the small molecular substrate affinity to a receptor (Hekmat et al, 2022).…”

Section: (F)mentioning

confidence: 99%

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Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Taherkhani

Hekmat

Piri

et al. 2022

Journal of Food Biochemistry

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Inhibition of tyrosinase activity can control fruit browning and preserve the flavor and nutritional value of food. The impacts of fulvic acid (FA) and humic acid (HA) on tyrosinase activity were investigated utilizing circular dichroism (CD) and fluorescence spectroscopy, molecular docking (MD), and molecular dynamics simulations. HA and FA demonstrated a mixed type of inhibition with K i 2.02 and 5.2 μM, respectively. The thermodynamic parameters displayed that the hydrogen bond and hydrophobic force play a major role in the FA-tyrosinase and HA-tyrosinase interaction, respectively.Fluorescence experiments demonstrated changes in tyrosinase tertiary structures.HA could not destroy the tyrosinase secondary structure significantly, however, FA has a significant influence on the tyrosinase secondary structure. The molecular dynamics findings demonstrated the minimal fluctuations and the lowest flexibility in the complex amino acids in the HA-tyrosinase and FA-tyrosinase interaction. Altogether, HA and FA could be utilized in food industries as an accessible natural source for tyrosinase inhibition. Practical applicationsRecently, the investigation of tyrosinase inhibitors from the biosphere for hindrance of undesired browning in the food industry has increased considerably. Mushroom tyrosinase is a suitable model for kinetic research owing to its availability as well as close conformational similarity to tyrosinase in a mammal. Natural sources and their effective compounds could have wonderful potential on tyrosinase activity and structure, thus, in this study, the interactions between tyrosinase and fulvic acid (FA) and Humic acid (HA) were investigated. Previously, it has been shown that HA and FA have antioxidant properties and they can improve the quality of food via retarding lipid oxidation. Altogether, further investigations are warranted to draw firm conclusions, HA and FA could be utilized in food industries not only as antioxidant agents but also as an accessible natural source for tyrosinase inhibition.

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Section: Resultsmentioning

confidence: 99%

{F}_{corr}={F}_{obs}\cdotp {10}^{\left({Ab}_{ex}+{Ab}_{em}\right)/2},

Section: Methodsmentioning

confidence: 99%

Section: The Intrinsic Fluorescence Measurementsmentioning

confidence: 99%

Section: Mechanism Of Fluorescence Quenchingmentioning

confidence: 99%

Section: (F)mentioning

confidence: 99%

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Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Taherkhani

Hekmat

Piri

et al. 2022

Journal of Food Biochemistry

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Exploring the binding mechanism and adverse toxic effects of chiral phenothrin to human serum albumin: Based on multi-spectroscopy, biochemical and computational approach

Gao

Bian

et al. 2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Wound Healing Accelerated by Silver Nanowire–Doped Methacryloyl Gelatin Dressings

Wang,

Zhu,

Hou

et al. 2024

ACS Appl. Nano Mater.

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Developing multifunctional hydrogel dressings with good biocompatibility and mechanical compatibility can accelerate skin healing and alleviate pain. Although hydrogel dressings can achieve a wound closure rate of nearly 90% in 15 days, there is room for improving their healing efficiency. Herein, we synthesized a novel hydrogel dressing by doping methacrylated gelatin (GelMA) with silver nanowires (AgNWs) via mechanical mixing and ultraviolet light curing. We abbreviated the GelMA/AgMWs hydrogels as GMAN x , with "x" indicating the initial amount of AgNWs (w/v). We found that GMAN 5 , with an AgNWs content of 0.5 mg/mL, had potent antibacterial activity (nearly 100% against Staphylococcus aureus and Escherichia coli), displayed good biocompatibility, and achieved a wound closure rate of 99.6% within 14 days (circular skin wounds with a diameter of 10 mm). The AgNWs not only improved the mechanical property of the hydrogel wound dressing but also made it conductive. Thanks to its excellent strain electrical response (gage factor (GF) = 2.3 under low strain conditions of 0−70%) and temperature electrical response (temperature coefficient of resistance (TCR) = 3.68%/°C within 30−40 °C), the GelMA 5 hydrogel dressing has potential applications for sensing changes in the wound.

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The effects of synthesized silver nanowires on the structure and esterase-like activity of human serum albumin and their impacts on human endometrial stem cells

Cited by 4 publications

References 64 publications

Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Exploring the binding mechanism and adverse toxic effects of chiral phenothrin to human serum albumin: Based on multi-spectroscopy, biochemical and computational approach

Wound Healing Accelerated by Silver Nanowire–Doped Methacryloyl Gelatin Dressings

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