The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro

Gentleman, Eileen; Fredholm, Yann C.; Jell, Gavin; Lotfibakhshaiesh, Nasrin; O’Donnell, Matthew; Hill, Robert G.; Stevens, Molly M.

doi:10.1016/j.biomaterials.2010.01.121

Cited by 537 publications

(395 citation statements)

References 36 publications

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“…When compared to growth medium (CTL), silicon ions were present in BG-conditioned media, and calcium content was increased. The reduced amount of phosphorous in the medium was likely due to the formation of a calcium phosphate layer on the BG surface, as previously described for several BG compositions [10]. No differences in ionic contents were found between the two BG, with the exception of the cobalt concentration.…”

Section: Incubation Of Cobalt-containing Bg Into Cell Culture Medium supporting

confidence: 73%

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Noor

Azevedo

Mohamad

et al. 2016

AIP Conference Proceedings

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Abstract.Vascularization is an important aspect of tissue regeneration. Hypoxia, low oxygen concentration, is a known stimulus for the release of vascular endothelial growth factors (VEGF) which play important roles in vascularization. The current study aimed to assess the effect of a cobalt-containing bioactive glass (BG) in stimulating hypoxia and promoting vascularization. To incorporate cobalt into BG, 1 mol% of calcium was substituting with cobalt, and this formulation was compared to the one without cobalt. Both BGs were processed via melt-derived method. The BG powders with particle size less than 38 μm were incubated with cell culture medium for 4 hours at 37°C on continuous rolling, and then the medium was filtered using 0.22 μm syringe filters. Prior to use, the BG-conditioned media were supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) antibiotic-antimycotic, and were allowed to equilibrate overnight inside a CO2 incubator. The conditioned media were used on human dental stem cells (stem cells from permanent (DPSC) and deciduous (SHED) teeth) and assessed for their capacity to stimulate the release of angiogenic factors from the cells. The results showed that cobalt ions were released from the cobalt-containing BG, following partial dissolution of the glasses in cell culture medium, and promoted VEGF release from the cells. In conclusion, the incorporation of cobalt in BG may have potential to be used for tissue regeneration by promoting vascularization through the activation of hypoxia pathway and the release of VEGF.

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Section: Incubation Of Cobalt-containing Bg Into Cell Culture Medium supporting

confidence: 73%

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Noor

Azevedo

Mohamad

et al. 2016

AIP Conference Proceedings

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“…under the trade name of NovaBone for orthopaedic or PerioGlas for periodontal applications. Strontium-substituted BGs have recently been shown to combine the known bone regenerative properties of BG with the anabolic and anticatabolic effects of Sr 2þ cations in vitro, making them of interest for bone regeneration in patients suffering from osteoporosis [5]. An ideal bone cement for treatment of vertebral compression fractures would combine the benefits of strontium-substituted BGs with handling properties allowing for minimally invasive delivery, and GICs based on strontium-containing BGs are of great interest for such applications.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal strontium-releasing injectable bone cements based on bioactive glasses

Brauer

Karpukhina

Kedia

et al. 2013

J. R. Soc. Interface.

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Strontium-releasing injectable bone cements may have the potential to prevent implant-related infections through the bactericidal action of strontium, while enhancing bone formation in patients suffering from osteoporosis. A meltderived bioactive glass (BG) series (SiO 2 -CaO-CaF 2 -MgO) with 0-50% of calcium substituted with strontium on a molar base were produced. By mixing glass powder, poly(acrylic acid) and water, cements were obtained which can be delivered by injection and set in situ, giving compressive strength of up to 35 MPa. Strontium release was dependent on BG composition with increasing strontium substitution resulting in higher concentrations in the medium. Bactericidal effects were tested on Staphylococcus aureus and Streptococcus faecalis; cell counts were reduced by up to three orders of magnitude over 6 days. Results show that bactericidal action can be increased through BG strontium substitution, allowing for the design of novel antimicrobial and bone enhancing cements for use in vertebroplasty or kyphoplasty for treating osteoporosis-related vertebral compression fractures.

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“…Bioglass ® has Federal Drug Administration (FDA) approval and is commercially available for several applications in hard tissue restoration, in products such as NovaBone ® , NovaMin ® , Perioglas ® and Vitoss-BA ® 21 . Several modified versions of 45S5 have been reported and different ions, such as strontium, magnesium or zinc, have been added to the initial composition with the aim of improving bone bonding and regeneration [22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of hypoxia-mimicking bioactive glasses for skeletal regeneration

et al. 2010

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The cellular response to hypoxia is vital for skeletal tissue development and regeneration. Numerous processes, including progenitor cell recruitment, proliferation and differentiation, are activated through the hypoxia pathway in a low oxygen pressure environment. Novel materials-based strategies designed to activate the hypoxia pathway are therefore of great interest for orthopaedic tissue engineering.Here resorbable bioactive glasses (BG) were designed to activate the hypoxia pathway by substitution of Co 2+ for Ca 2+ in the BG and substitution of Co 2+ and Ca 2+ /+2Na + for Si 4+ . This allowed for the controlled release of cobalt, whilst controlling BG bioactivity (apatite-forming ability). Two series of soda-lime-phosphosilicate glasses were manufactured with increasing concentrations of cobalt. Compositions were calculated to maintain constant network connectivity (2.13) by considering cobalt as an intermediate oxide in the first series, and as a network modifier in the second series. Mg 2+ and Zn 2+ were added to one of the Co 2+ -containing glasses to inhibit HCA formation since delay or inhibition of HCA formation is essential for the use of BG in soft tissues, as is the case of cartilage.Cobalt was present in both the silicate and phosphate phases of the BG. In addition, evidence was found for it playing a dual role in the silicate phase, acting as both an intermediate oxide and taking part in the network as well as being a network modifying oxide. Consistent with an intermediate oxide role, the presence of cobalt in the BG was shown to decrease ion release. HCA formation was delayed with cobalt additions. Mg 2+ and Zn 2+ further delayed HCA formation.Cobalt release was found to be proportional to cobalt content of the BGs enabling the controlled delivery of cobalt in therapeutic active doses.

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The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro

Cited by 537 publications

References 36 publications

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Hypoxia-mimicking bioactive glass regenerative effects on dental stem cells

Bactericidal strontium-releasing injectable bone cements based on bioactive glasses

Synthesis and characterization of hypoxia-mimicking bioactive glasses for skeletal regeneration

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