The effects of stress, cortisol administration and cortisol inhibition on black sea bass (Centropristis striata) sex differentiation

Miller, Kelsie A.; Kenter, Linas W.; Breton, Timothy S.; Berlinsky, David L.

doi:10.1016/j.cbpa.2018.10.009

Cited by 28 publications

(12 citation statements)

References 64 publications

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“…The hypothalamic–pituitary–adrenal (HPA) axis in reptiles, birds and mammals or inter‐renal (HPI) axis in fish and amphibians has a role in sex determination in a range of taxa [see reviews in Goikoetxea, Todd & Gemmell () and Geffroy & Douhard ()]. Among gonochoristic (single‐sex) fish, cortisol‐mediated sex determination in response to temperature is well supported by experimental application of cortisol (Hattori et al ., ; Hayashi et al ., ; Castañeda Cortés et al ., ; Miller et al ., ). Cortisol has not yet been experimentally demonstrated to be a mediator of sex change in sequentially hermaphroditic teleost fish, but transcriptomic evidence suggests cortisol upregulation, supporting a role for the HPI axis in the repression of aromatase and the regulation of downstream epigenetic effectors of gene regulation (Fernandino et al ., ; Solomon‐Lane, Crespi & Grober, ; Goikoetxea et al ., ; Todd et al ., ).…”

Section: Connections Between Care Status and Sex Determinationmentioning

confidence: 97%

Cellular calcium and redox regulation: the mediator of vertebrate environmental sex determination?

et al. 2020

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Many reptiles and some fish determine offspring sex by environmental cues such as incubation temperature. The mechanism by which environmental signals are captured and transduced into specific sexual phenotypes has remained unexplained for over 50 years. Indeed, environmental sex determination (ESD) has been viewed as an intractable problem because sex determination is influenced by a myriad of genes that may be subject to environmental influence. Recent demonstrations of ancient, conserved epigenetic processes in the regulatory response to environmental cues suggest that the mechanisms of ESD have a previously unsuspected level of commonality, but the proximal sensor of temperature that ultimately gives rise to one sexual phenotype or the other remains unidentified. Here, we propose that in ESD species, environmental cues are sensed by the cell through highly conserved ancestral elements of calcium and redox (CaRe) status, then transduced to activate ubiquitous signal transduction pathways, or influence epigenetic processes, ultimately to drive the differential expression of sex genes. The early evolutionary origins of CaRe regulation, and its essential role in eukaryotic cell function, gives CaRe a propensity to be independently recruited for diverse roles as a 'cellular sensor' of environmental conditions. Our synthesis provides the first cohesive mechanistic model connecting environmental signals and sex determination pathways in vertebrates, providing direction and a framework for developing targeted experimentation.

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Section: Connections Between Care Status and Sex Determinationmentioning

confidence: 97%

Cellular calcium and redox regulation: the mediator of vertebrate environmental sex determination?

et al. 2020

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“…Exogenous cortisol feed has also been shown to affect sex change in sequential protogynous hermaphroditic species. In black sea bass, Centropristis striata , females were fed a diet containing 300 mg kg −1 cortisol for 84 days, which resulted in significantly fewer females and more intersex fish than control treatments (Miller, Kenter, Breton, & Berlinsky, 2019). In the three‐spot wrasse, female fish (with a terminal phase male present to block natural sex change) were fed 200 μg g −1 and 1,000 μg g −1 cortisol diets for 6 weeks, and the 1,000 μg g −1 dosage resulted in a significant masculinization of the populations (Nozu & Nakamura, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary cortisol administration on sex ratios in dwarf gouramis and rosy barbs

Ramee

Lipscomb

DiMaggio

2020

J World Aquaculture Soc

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“…In addition, treatment with the stress hormone CORT was predicted to induce oocyte degeneration through the inhibition of aromatase (15), and it was hypothesised that this effect alone may trigger the female-to-male gonadal transformation. A strong effect of CORT, the main glucocorticoid in fish, has been linked to fish sex determination and sex change in several studies (16–20). This is the first study to evaluate the effects of CORT in an in vitro gonadal culture system of a sex-changing fish.…”

Section: Introductionmentioning

confidence: 99%

Anin vitroovarian explant culture system to examine sex change in a hermaphroditic fish

Goikoetxea

Damsteegt

Todd

et al. 2020

Preprint

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Many teleost fishes undergo natural sex change, and elucidating the physiological and molecular controls of this process offers unique opportunities not only to develop methods of controlling sex in aquaculture settings, but to better understand vertebrate sexual development more broadly. Induction of sex change in some sequentially hermaphroditic or gonochoristic fish can be achieved in vivo through social manipulation, inhibition of aromatase activity, and steroid treatment. However, the induction of sex change in vitro has been largely unexplored. In this study, we established an in vitro culture system for ovarian explants in serum-free medium for a model sequential hermaphrodite, the New Zealand spotty wrasse (Notolabrus celidotus). This culture technique enabled evaluating the effect of various treatments with 17β-estradiol (E2), 11-ketotestosterone (11KT) or cortisol (CORT) on spotty wrasse ovarian architecture for 21 days. A quantitative approach to measuring the degree of ovarian atresia within histological images was also developed, using pixel-based machine learning software. Ovarian atresia likely due to culture was observed across all treatments including no-hormone controls, but was minimised with treatment of at least 10 ng/mL E2. Neither 11KT nor CORT administration induced proliferation of spermatogonia (i.e. sex change) in the cultured ovaries indicating culture beyond 21 days may be needed to induce sex change in vitro. The in vitro gonadal culture and analysis systems established here enable future studies investigating the paracrine role of sex steroids, glucocorticoids and a variety of other factors during gonadal sex change in fish.

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The effects of stress, cortisol administration and cortisol inhibition on black sea bass (Centropristis striata) sex differentiation

Cited by 28 publications

References 64 publications

Cellular calcium and redox regulation: the mediator of vertebrate environmental sex determination?

Cellular calcium and redox regulation: the mediator of vertebrate environmental sex determination?

Effects of dietary cortisol administration on sex ratios in dwarf gouramis and rosy barbs

Anin vitroovarian explant culture system to examine sex change in a hermaphroditic fish

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