The effects of soybean lipoxygenase-1 on chloroplasts from wheat

Köckritz, Annerose; Schewe, Tankred; Hieke, B.; Hass, Werner

doi:10.1016/s0031-9422(00)80732-3

Cited by 15 publications

(5 citation statements)

References 12 publications

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“…65,71,143,188) or lipoxygenase in the presence of linolenic acid (e.g. 68). Chl is also bleached when chloroplast membranes (11,76,82) or isolated photosystem preparations (77) are incubated in the presence of unsaturated fatty acids.…”

Section: Chlorophyll-bleaching Enzymes Of Unknown Functionsmentioning

confidence: 99%

Chlorophyll Degradation

Matile

Hörtensteiner

Thomas

1999

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

501

358

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Although the loss of green color in senescent leaves and ripening fruits is a spectacular natural phenomenon, research on chlorophyll breakdown has been largely neglected until recently. This review summarizes knowledge about the fate of chlorophyll in degreening tissues that has been gained during the past few years. Structures of end- and intermediary products of degradation as well as the biochemistry of the porphyrin-cleaving reaction have been elucidated. The intracellular localization of the catabolic pathway is particularly important in the regulation of chlorophyll breakdown. None of the genes encoding the related catabolic enzymes has so far been isolated, which makes chlorophyll degradation an area of opportunity for future research.

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Section: Chlorophyll-bleaching Enzymes Of Unknown Functionsmentioning

confidence: 99%

Chlorophyll Degradation

Matile

Hörtensteiner

Thomas

1999

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

501

358

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“…This process was proposed as the general mechanism of peroxidative destruction of thylakoid membranes in isolated chloroplasts and photosynthesis inhibition. The wide distribution of lipoxygenase in green plants also suggests its possible involvement in the developmental inactivation and breakdown of chloroplasts (Kockritz et al, 1985).…”

Section: Ultrastructure and Lipid Alterations Induced By Cadmium In Tmentioning

confidence: 99%

Ultrastructure and Lipid Alterations Induced by Cadmium in Tomato (Lycopersicon esculentum) Chloroplast Membranes

Zarrouk²,

et al. 2005

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The effects of cadmium (Cd) uptake on ultrastructure and lipid composition of chloroplasts were investigated in 28-day-old tomato plants (Lycopersicon esculentum var. Ibiza F1) grown for 10 days in the presence of various concentrations of CdCl2. Different growth parameters, lipid and fatty acid composition, lipid peroxidation, and lipoxygenase activity were measured in the leaves in order to assess the involvement of this metal in the generation of oxidative stress. We first observed that the accumulation of Cd increased with external metal concentration, and was considerably higher in roots than in leaves. Cadmium induced a significant inhibition of growth in both plant organs, as well as a reduction in the chlorophyll and carotenoid contents in the leaves. Ultrastructural investigations revealed that cadmium induced disorganization in leaf structure, essentially marked by a lowered mesophyll cell size, reduced intercellular spaces, as well as severe alterations in chloroplast fine structure, which exhibits disturbed shape and dilation of thylakoid membranes. High cadmium concentrations also affect the main lipid classes, leading to strong changes in their composition and fatty acid content. Thus, the exposure of tomato plants to cadmium caused a concentration-related decrease in the fatty acid content and a shift in the composition of fatty acids, resulting in a lower degree of fatty acid unsaturation in chloroplast membranes. The level of lipid peroxides and the activity of lipoxygenase were also significantly enhanced at high Cd concentrations. These biochemical and ultrastructural changes suggest that cadmium, through its effects on membrane structure and composition, induces premature senescence of leaves.

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“…Furthermore, Tab the wide distribution of lipoxygenases in green plants also suggests its role In the developmental inactivation and break-down of chloroplasts (H. Hertel 1982, Thesis, Univ. of Humboldt, Berlin, Germany; Kockritz et al 1985).…”

Section: Lipoxygenase Activity In Cd^^'^-treated Seedlingsmentioning

confidence: 99%

Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation

Somashekaraiah¹,

Padmaja²,

Prasad³

1992

Physiol Plant

137

140

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Phytotoxicity of cadmium ions on germinating seedlings of mung bean {Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation -Physiol. Plant. 85: 85-89.Germinating seedlings of mung bean {Phaseolus vulgaris L. cv. K-16) were treated with different concentrations of cadmium acetate (10, 50 and 100 nM), Cd'* lowered the chlorophyll and heme levels. The level of lipid peroxides were higher on day 3 than on day 6. However, Cd*^ treatment significantly enhanced the level of lipid peroxides. Similarly, a dose-dependent induction of lipoxygenase (EC 1.13.11.12) activity was observed with Cd-* treatment. Further, the activities of antioxidant enzymes such as superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) were decreased. Our results suggest that lipoxygenase-mediated accumulation of lipid peroxides on the one hand and inhibition of free radical scavenging enzymes like superoxide dismutase and catalase on the other caused a pronounced reduction in the chlorophyll and heme levels of the seedlings. The experiments conducted on the effect of Cd"^ on dark-grown seedlings did not conform with the result of light-grown seedlings. Though chlorophyll and heme levels decreased in a dose-dependent manner, no accumulation of lipid peroxides was observed. Suggesting that the inhibition of chlorophyll synthesis by Cd^* is achieved both by reaction with constituent biosynthetic enzymes as well as peroxide-mediated degradation.

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The effects of soybean lipoxygenase-1 on chloroplasts from wheat

Cited by 15 publications

References 12 publications

Chlorophyll Degradation

Chlorophyll Degradation

Ultrastructure and Lipid Alterations Induced by Cadmium in Tomato (Lycopersicon esculentum) Chloroplast Membranes

Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation

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