The effects of short-term exposition to Cd, excess Cu ions and jasmonate on oxidative stress appearing in Arabidopsis thaliana

Maksymiec, Waldemar; Krupa, Zbigniew

doi:10.1016/j.envexpbot.2005.05.006

Cited by 192 publications

(120 citation statements)

References 54 publications

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“…Elevated levels of ROS can cause cellular damage, but are also important in stress signalling (Mittler et al 2004). Multiple authors have demonstrated responses at the biochemical-physiological level and on signal transduction pathways induced by exposure to relatively high (acute) metal concentrations in plants (Jonak et al 2004;Maksymiec andKrupa, 2006, Yeh et al 2007), whereas in our studies, we expose Arabidopsis thaliana to lower sub-lethal concentrations of Cu or Cd. At these low exposures to metals no visible changes to the plant can be detected, but a number of changes are taking place at the cellular level.…”

Section: Introductionmentioning

confidence: 73%

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Remans

Smeets

Opdenakker

et al. 2008

Planta

302

225

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Accurate quantification by real-time RT-PCR relies on normalisation of the measured gene expression data. Normalisation with multiple reference genes is becoming the standard, but the best reference genes for gene expression studies within one organism may depend on the applied treatments or the organs and tissues studied. Ideally, reference genes should be evaluated in all experimental systems. A number of candidate reference genes for Arabidopsis have been proposed, which can be used as a starting point to evaluate their expression stability in individual experimental systems by available computer algorithms like geNorm and NormFinder. Using this approach, we identified the best three reference genes from a set of ten candidates -that included three traditional "housekeeping" genes -for normalisation of gene expression when roots and leaves of Arabidopsis thaliana are exposed to cadmium (Cd) and copper (Cu). The expression stabilities of AT5G15710 (F-box protein), AT2G28390 (SAND family protein), and AT5G08290 (mitosis protein YLS8) were the highest when considering the effect to the roots and shoots of Cd and Cu treatments. Even though the effect of Cd and excess Cu on the plants is very different, the same best reference genes were identified when considering Cd or Cu treatments separately. This suggests that these three genes may also be suitable when studying the gene expression after exposure of Arabidopsis thaliana to increased concentrations of other metals.

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Section: Introductionmentioning

confidence: 73%

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Remans

Smeets

Opdenakker

et al. 2008

Planta

302

225

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“…In contrast, JA was found to increase metal biosorption and ROS generation in the green microalga Chlorella vulgaris (Chlorophyceae) exposed to excessive Cd, Cu, or Pb (Piotrowska-Niczyporuk et al, 2012). Moreover, ROS production was triggered by JA in Arabidopsis treated with Cu or Cd (Maksymiec and Krupa, 2006). However, it has also been reported that JA-induced ROS is mediated by the oxidative status of GSH and that JA induced the expression of GSH metabolic genes (Xiang and Oliver, 1998;Mhamdi et al, 2010).…”

Section: Ethylene and Its Cross Talk With Other Hormones And Signalinmentioning

confidence: 96%

Role of Ethylene and Its Cross Talk with Other Signaling Molecules in Plant Responses to Heavy Metal Stress

et al. 2015

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Excessive heavy metals (HMs) in agricultural lands cause toxicities to plants, resulting in declines in crop productivity. Recent advances in ethylene biology research have established that ethylene is not only responsible for many important physiological activities in plants but also plays a pivotal role in HM stress tolerance. The manipulation of ethylene in plants to cope with HM stress through various approaches targeting either ethylene biosynthesis or the ethylene signaling pathway has brought promising outcomes. This review covers ethylene production and signal transduction in plant responses to HM stress, cross talk between ethylene and other signaling molecules under adverse HM stress conditions, and approaches to modify ethylene action to improve HM tolerance. From our current understanding about ethylene and its regulatory activities, it is believed that the optimization of endogenous ethylene levels in plants under HM stress would pave the way for developing transgenic crops with improved HM tolerance.

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“…In the leaves, decreased SOD, CAT, and POD activity in both castor cultivars after Cd treatment (Fig. 3) may be attributed to the lack of an effective antioxidant enzyme system (Liu et al, 2007) or the excessive production of oxygen free radicals (Maksymiec and Krupa, 2006;Li et al, 2011a). Nouairi et al (2009) showed that SOD and CAT activity in the leaves of Brassica napus decreased approximately 40% and 37%, respectively, to that of the control, and GPX activity was drastically reduced ( $ 57%) in the leaves of Brassica juncea plants, at a 50 mM Cd dose.…”

Section: Antioxidant Enzymesmentioning

confidence: 98%

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Zhang

Guo

Yang

et al. 2015

Ecotoxicology and Environmental Safety

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a b s t r a c tThe aims of this study were: (1) the study of cadmium (Cd) accumulation and toxicity in different castor cultivars (Ricinus communis L.); (2) to investigate changes in antioxidant enzymatic activities and the subcellular distribution of Cd in young and old leaves from two different castor cultivars, after exposure to two different Cd concentrations, and explore the underlying mechanism of Cd detoxification focusing on antioxidant enzymes and subcellular compartmentalization. The Cd concentration, toxicity, and subcellular distribution, as well as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were measured in Zibo-3 and Zibo-9 cultivars after exposure to two different concentrations of Cd (2 mg/L and 5 mg/L) for 10 days. This research revealed Cd accumulation characteristics in castor are root4 stem4 young leaf 4old leaf. Castor tolerance was Cd dose exposure and the cultivars themselves dependent. Investigation of subcellular Cd partitioning showed that Cd accumulated mainly in the heat stable protein (HSP) and cellular debris fractions, followed by the Cd rich granule (MRG), heat denatured protein (HDP), and organelle fractions. With increasing Cd concentration in nutrient solution, the decreased detoxified fractions (BDM) and the increased Cd-sensitive fractions (MSF) in young leaves may indicate the increased Cd toxicity in castor cultivars. The BDM-Cd fractions or MSF-Cd in old leaves may be linked with Cd tolerance of different cultivars of castor. The antioxidant enzymes that govern Cd detoxification were not found to be active in leaves. Taken together, these results indicate Cd tolerance and toxicity in castor can be explained by subcellular partitioning.Crown

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The effects of short-term exposition to Cd, excess Cu ions and jasmonate on oxidative stress appearing in Arabidopsis thaliana

Cited by 192 publications

References 54 publications

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Role of Ethylene and Its Cross Talk with Other Signaling Molecules in Plant Responses to Heavy Metal Stress

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

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