The effects of short-chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype

Comalada, Mònica; Bailón, Elvira; Haro, Oscar de; Lara-Villoslada, Federico; Xaus, Jordi; Zarzuelo, Antonio; Galvez, Julio

doi:10.1007/s00432-006-0172-y

Cited by 21 publications

(23 citation statements)

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“…Further, B. coagulans MTCC 5856 produced notable amount of acetic acid, propionic acid and also a minimal amount of butyric acid while fermenting with CSF as the sole source of nutrition. Propionic acid and butyrate play an important role in cell cycle processes such as proliferation, differentiation and apoptosis (Comalada et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Cranberry seed fibre: a promising prebiotic fibre and its fermentation by the probiotic Bacillus coagulans MTCC 5856

Majeed

Nagabhushanam

Sivakumar

et al. 2018

Int J of Food Sci Tech

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SummaryCranberry, a versatile fruit, is known for nutritional as well as medicinal properties due to the presence of bioactive compounds. However, cranberry fruit has not been fully explored for its prebiotic potential. Therefore, this study was conducted to evaluate prebiotic potential of cranberry seed fibre (CSF) and also evaluate its fermentability by the probiotic strain Bacillus coagulans MTCC 5856. The resistance to gastric acid and porcine pancreatic enzymatic (PPE) hydrolysis of CSF was investigated using an in vitro model. It was found that CSF was resistant to gastric acid and also nondigestible to PPE hydrolysis. CSF as sole nutrition source was evaluated for the fermentability by B. coagulans MTCC 5856. A significant amount of short-chain fatty acids was produced by the B. coagulans MTCC 5856 while fermenting cranberry fibres anaerobically. CSF supported the growth of B. coagulans MTCC 5856 and also inhibited the growth of E. coli ATCC 25922 when cocultured in an anaerobic environment. CSF from the cranberry fruit exhibited prebiotic potential and also found to be fermentable by B. coagulans MTCC 5856. This study provided the scientific evidence of CSF as a prebiotic fibre and also its suitability with the probiotic B. coagulans MTCC 5856 for an ideal synbiotic preparation.

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Section: Discussionmentioning

confidence: 99%

Cranberry seed fibre: a promising prebiotic fibre and its fermentation by the probiotic Bacillus coagulans MTCC 5856

Majeed

Nagabhushanam

Sivakumar

et al. 2018

Int J of Food Sci Tech

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“…For example, propionate has similar properties to butyrate, a result that is consistent with current literature. 14,16,[30][31][32] It is well established that SCFA of three to five carbons in length are active, whereas the two-carbon SCFA acetate has a diminished effect on cell growth, apoptosis and histone hyperacetylation in colorectal adenocarcinoma cell lines such as HT-29, 14,16,30,32 HCT-116 16,31 and VACO-5 cells. 31 The common structural properties of the compounds of interest (i.e.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of butyrate analogues in HT‐29 cancer cells

Ooi

Good

Williams

et al. 2010

Clin Exp Pharma Physio

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1. Butyrate is a well known product of starch fermentation by colonic bacteria and is of interest owing to its ability to induce in vitro apoptosis and cell differentiation, as well as to inhibit cell growth in colorectal and other cancer cells. Synthetic analogues of butyrate may also possess cellular activities in a variety of cultured cells. The aim of the present study was to evaluate the effects of butyrate analogues on apoptosis, proliferation and histone deacetylase (HDAC) activity in HT-29 colorectal cancer cells. In addition, the effects of these analogues on lactate dehydrogenase leakage, as a measure of non-specific cytotoxicity, were evaluated in HT-29 cells. 2. Of the 26 analogues examined, four (propionate, 4-benzoylbutyrate, 4-(4-aminophenyl)butyrate and benzyloxyacetate) exhibited comparable effects to butyrate. Interestingly, no activity was noted for compounds carrying amino, hydroxyl or methyl substitutions at the 2-, 3- or 4-position of the aliphatic moiety of butyrate. 3. In conclusion, chemical changes to the structure of butyrate can significantly modify the biological activity assayed in HT-29 colorectal cancer cells in vitro.

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“…Butyrate inhibits the growth of cancerous colonic cells, but not of normal colonocytes and, depending on the concentration, it actually increases the proliferation of this latter cell type. 12,21 The mechanism proposed for this difference in susceptibility to butyrate involves the so-called Warburg effect. In brief, cancerous cells present a metabolic shift from oxidative metabolism to glycolysis Figure 1 Cellular signaling pathways activated by the short-chain fatty acids.…”

Section: Epithelial Cellsmentioning

confidence: 99%

Regulation of immune cell function by short‐chain fatty acids

et al. 2016

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Short-chain fatty acids (SCFAs) are bacterial fermentation products, which are chemically composed by a carboxylic acid moiety and a small hydrocarbon chain. Among them, acetic, propionic and butyric acids are the most studied, presenting, respectively, two, three and four carbons in their chemical structure. These metabolites are found in high concentrations in the intestinal tract, from where they are uptaken by intestinal epithelial cells (IECs). The SCFAs are partially used as a source of ATP by these cells. In addition, these molecules act as a link between the microbiota and the immune system by modulating different aspects of IECs and leukocytes development, survival and function through activation of G protein coupled receptors (FFAR2, FFAR3, GPR109a and Olfr78) and by modulation of the activity of enzymes and transcription factors including the histone acetyltransferase and deacetylase and the hypoxia-inducible factor. Considering that, it is not a surprise, the fact that these molecules and/or their targets are suggested to have an important role in the maintenance of intestinal homeostasis and that changes in components of this system are associated with pathological conditions including inflammatory bowel disease, obesity and others. The aim of this review is to present a clear and updated description of the effects of the SCFAs derived from bacteria on host immune system, as well as the molecular mechanisms involved on them.

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The effects of short-chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype

Cited by 21 publications

References 0 publications

Cranberry seed fibre: a promising prebiotic fibre and its fermentation by the probiotic Bacillus coagulans MTCC 5856

Cranberry seed fibre: a promising prebiotic fibre and its fermentation by the probiotic Bacillus coagulans MTCC 5856

Efficacy of butyrate analogues in HT‐29 cancer cells

Regulation of immune cell function by short‐chain fatty acids

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