The effects of shear stress and met astatic phenotype on the detachment of transformed cells

Cezeaux, J.L.; Austin, Vince; Hosseinipour, Mina C.; Ward, Ken; Zimmer, Stephen G.

doi:10.3233/bir-1991-283-411

Cited by 9 publications

(6 citation statements)

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“…If such effects are also occurring in tumors between parenchymal cells, they could also be facilitating the dissemination of tumor cells and potentially exacerbating the metastatic process. Cezeaux et al [33], for example, carried out studies using a parallel plate flow chamber looking at the effects of shear stress on cell detachment. They showed that the induced stress significantly increased detachment of cells when using highly metastatic ras-transformed cells compared to nonmetastatic normal cells.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pulsed high intensity focused ultrasound exposures on metastasis in a murine model

Hancock

Dreher

Crawford

et al. 2009

Clin Exp Metastasis

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High intensity focused ultrasound (HIFU) may be employed in two ways: continuous exposures for thermal ablation of tissue (>60°C), and pulsed-exposures for non-ablative effects, including low temperature hyperthermia (37–45°C), and non thermal effects (e.g. acoustic cavitation and radiation forces). Pulsed-HIFU effects may enhance the tissue's permeability for improved delivery of drugs and genes, for example, by opening up gaps between cells in the vasculature and parenchyma. Inducing these effects may improve local targeting of therapeutic agents, however; concerns exist that pulsed exposures could theoretically also facilitate dissemination of tumor cells and exacerbate metastases. In the present study, the influence of pulsed-HIFU exposures on increasing metastatic burden was evaluated in a murine model with metastatic breast cancer. A preliminary study was carried out to validate the model and determine optimal timing for treatment and growth of lung metastases. Next, the effect of pulsed-HIFU on the metastatic burden was evaluated using quantitative image processing of whole-lung histological sections. Compared to untreated controls (2/15), a greater number of mice treated with pulsed-HIFU were found to have lungs “overgrown” with metastases (7/15), where individual metastases grew together such that they could not accurately be counted. Furthermore, area fraction of lung metastases (area of metastases/area of lungs) was ~30% greater in mice treated with pulsed-HIFU; however, these differences were not statistically significant. The present study details the development of an animal model for investigating the influence of interventional techniques or exposures (such as pulsed HIFU) on metastatic burden.

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Section: Discussionmentioning

confidence: 99%

Evaluation of pulsed high intensity focused ultrasound exposures on metastasis in a murine model

Hancock

Dreher

Crawford

et al. 2009

Clin Exp Metastasis

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“…14,15 This apparatus consisted of 2 parallel glass slides and a middle silicone gasket separating them. The cultured corneal endothelial cells (3 3 10 5 ) were seeded on a glass slide and allowed to attach for 1 or 3 hours.…”

Section: Shear Stress Modelmentioning

confidence: 99%

“…Shear stress is known to induce cell detachment and various cytokine expressions that are postulated to lead to vascular damage. 13,14 In the present study, we investigated the effect of shear stress on attachment of cultured corneal endothelial cells. Furthermore, we calculated the possible shear stress on corneal endothelial cell layer in a virtual model of laser iridotomy.…”

mentioning

confidence: 99%

Effect of Shear Stress on Attachment of Corneal Endothelial Cells in Association With Corneal Endothelial Cell Loss After Laser Iridotomy

Kaji¹,

Oshika²,

Usui³

et al. 2005

Cornea

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“…It has been shown that increasing shear stress by enhancing fluid flow can result in increasing the rate of detachment of fibroblasts or various types of tumor cells from cell aggregates or monolayers (69). This ability to detach from a cell mass may be related to the metastatic potential of transformed cells (70).…”

Section: Biophysical Factors Influencing Adhesive Interactionsmentioning

confidence: 99%

Tumor cell adhesion under hydrodynamic conditions of fluid flow

Haier

Nicolson

2001

APMIS

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Current evidence indicates that tumor cell adhesion to the microvasculature in host organs during formation of distant metastases is a complex process involving various types of cell adhesion molecules. Recent results have shown that stabilization of tumor cell adhesion to the microvascular vessel wall is a very important step for successful tumor cell migration and colonization of host organs. We are beginning to understand the influences of fluid flow and local shear forces on these adhesive interactions and cellular responses within the circulation. Mechanosensory molecules or molecular complexes can transform shear forces acting on circulating tumor cells into intracellular signals and modulate cell signaling pathways, gene expression and other cellular functions. Flowing tumor cells can interact with microvascular endothelial cells mediated mainly by selectins, but the strength of these bonds is relatively low and not sufficient for stable cell adhesions. Integrin-mediated tumor cell adhesion and changes in the binding affinity of these adhesion molecules appear to be required for stabilized tumor cell adhesion and subsequent cell migration into the host organ. Failure of the conformational affinity switch in integrins results in breaking of these bonds and recirculation or mechanical damage of the tumor cells. Various cell signaling molecules, such as focal adhesion kinase, pp60src or paxillin, and cytoskeletal components, such as actin or microtubules, appear to be required for tumor cell adhesion and its stabilization under hydrodynamic conditions of fluid flow.

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The effects of shear stress and met astatic phenotype on the detachment of transformed cells

Cited by 9 publications

References 0 publications

Evaluation of pulsed high intensity focused ultrasound exposures on metastasis in a murine model

Evaluation of pulsed high intensity focused ultrasound exposures on metastasis in a murine model

Effect of Shear Stress on Attachment of Corneal Endothelial Cells in Association With Corneal Endothelial Cell Loss After Laser Iridotomy

Tumor cell adhesion under hydrodynamic conditions of fluid flow

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