The effects of selected phytochemicals on biofilm formed by five methicillin-resistant <i>Staphylococcus aureus</i>

Kot, Barbara; Wierzchowska, Kamila; Grużewska, Agata; Lohinau, Dzmitry

doi:10.1080/14786419.2017.1340282

Cited by 38 publications

(33 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, chemical compositions and major compounds of essential oils vary in different habitats, and their bioactivity is closely associated with changes in their composition. Cinnamic aldehyde, for example, showed MIC ranging from 0.5 to 1000 μ g/mL against 20 strains of P. aeruginosa [33] and therapeutic potential by inhibiting infections related to biofilm production by S. aureus [34]. The turmerone compound found in C. longa demonstrated activity against E. coli [35] while showed no activity against Gram-positive bacterial strains [36].…”

Section: Discussionmentioning

confidence: 99%

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Teles

Rosa

Mouchrek

et al. 2019

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The resistance mechanisms of bacteria and protozoans have evidenced the need of discover new compounds with potential pharmaceutical activity against pathogenic microorganisms. Medicinal plants have been for centuries a promising alternative as sources of new drugs. The objective of this work was to evaluate the chemical composition, antimicrobial and antileishmanial activities of Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa essential oils. Chemical analysis was performed by gas chromatography-mass spectrometry. Antimicrobial activity was performed by disk diffusion and minimum inhibitory concentration (MIC) test. Antileishmanial activity was performed against antipromastigote and intracellular amastigote of Leishmania amazonensis. Cytotoxic and nitrite production were realized in BALB/c peritoneal macrophages. The major compounds of the essential oils were cinnamic aldehyde (46.30%) in C. zeylanicum, cis-p-menth-2-en-1-ol (33.88%) and linalyl acetate (13.90%) in O. vulgare, and turmerone (55.43%) in C. longa. The MIC showed significant antimicrobial activity of C. longa essential oil against S. aureus (83.3 ± 14.43 µg/mL). Antipromastigote activity showed IC50 values >500 µg/mL to C. zeylanicum, 308.4 ± 1.402 µg/mL to O. vulgare, and 405.5 ± 1.119 µg/mL to C. longa essential oil. Activity against intracellular amastigote of L. amazonensis showed IC50 of 63.3 ± 1.369 µg/mL and cytotoxic was not observed, resulting in selectivity index higher than 15.79 to parasite. C. longa essential oil decreased nitrite production in peritoneal macrophages, but not in Leishmania-infected cells. The chemical composition of the three essential oils is directly associated to its potential biological action, as the antimicrobial activity. C. longa presented a potent antileishmanial activity against promastigote and intracellular amastigote of L. amazonensis, although this activity is not linked to nitric oxide, since C. longa essential oil inhibits its production.

show abstract

Section: Discussionmentioning

confidence: 99%

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Teles

Rosa

Mouchrek

et al. 2019

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…These results indicate that TC is also an efficient inhibitor of MRSA adhesion to major components of the extracellular matrix such as elastin and laminin, which prevents dissemination of staphylococcal cells and the initiation of host tissue colonization. TC is widely known for its antibacterial properties [23][24][25][26][27][28][29][30][31] but to our knowledge, the influence of this compound on the expression of genes associated with biofilm formation by S. aureus was not yet investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Each test was repeated three times. For the purpose of determining the MBEC, TSB with 0.5% glucose, containing tested TC concentrations, was inoculated in triplicate, in a volume of 200 µL in the wells with established biofilm and incubated for 24 h at 37 • C. Biofilm formation assay was performed as described by Kot et al [25]. Subsequently, the medium was removed, and the biofilms were washed twice with 250 µL of sterile PBS.…”

Section: Determination Of Minimum Biofilm Inhibition Concentrations (mentioning

confidence: 99%

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Kot

Sytykiewicz

Sprawka

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

The effects of trans-cinnamaldehyde (TC) on transcriptional profiles of biofilm-associated genes and the metabolic activity of two methicillin-resistant Staphylococcus aureus (MRSA) strains showing a different degree of adherence to polystyrene, were evaluated. Metabolic activity of S. aureus in biofilm was significantly decreased in the presence of TC at 1/2 minimum biofilm inhibition concentration (MBIC). Expression levels of the genes encoding laminin binding protein (eno), elastin binding protein (ebps) and fibrinogen binding protein (fib) in the presence of TC at 1/2 MBIC were lower than in untreated biofilm in both the weakly and strongly adhering strain. The highest decrease of expression level was observed in case of fib in the strongly adhering strain, in which the amount of fib transcript was 10-fold lower compared to biofilm without TC. In the presence of TC at 1/2 MBIC after 3, 6, 8 and 12 h, the expression level of icaA and icaD, that are involved in the biosynthesis of polysaccharide intercellular adhesin, was above half lower in the weakly adhering strain compared to biofilm without TC. In the strongly adhering strain the highest decrease in expression of these genes was observed after 3 and 6 h. This study showed that TC is a promising anti-biofilm agent for use in MRSA biofilm-related infections.

show abstract

“…Antimicrobial activity against multiresistant clinical isolates of 11 pathogenic bacteria of a carvacrol chemotype of Lavandula coronopifolia was assessed . In a recent study, Thyme EO is able to reduce of 59.7–85.0 % biofilm biomass formed by methicillin resistant Staphylococcus aureus (MRSA) strains, while Lavandula multifida L. EO showed anti‐MRSA activity since the inhibition diameters reached 27 mm and MIC s were lower than 0.1 μg/mL in a study against 14 strains . Synergistic effects of sub‐inhibitory concentrations of Salvia officinalis EO and different antibiotics against MRSA were also evaluated by Milenković et al .…”

Section: Essential Oils: Very Old ‘Guys’ With Great Prospectsmentioning

confidence: 99%

“…Antimicrobial activity against multiresistant clinical isolates of 11 pathogenic bacteria of a carvacrol chemotype of Lavandula coronopifolia was assessed. [280] In a recent study, Thyme EO is able to reduce of 59.7 -85.0 % biofilm biomass formed by methicillin resistant Staphylococcus aureus (MRSA) strains, [281] while Lavandula multifida L. EO showed anti-MRSA activity since the inhibition diameters reached 27 mm and MICs were lower than 0.1 μg/mL in a study against 14 strains. [282] Synergistic effects of sub-inhibitory concentrations of Salvia officinalis EO and different antibiotics against MRSA were also evaluated by Milenković et al [283] Authors highlighted the presence of different interactions between tested oil and antibiotics from synergistic to antagonistic on different clinical isolates of MRSA suggesting the necessity for systematic in vitro studies of interactions of EOs and antibiotics to reveal any undesirable combinations.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

New Tricks for Old Guys: Recent Developments in the Chemistry, Biochemistry, Applications and Exploitation of Selected Species from the Lamiaceae Family

Napoli

Siracusa

Ruberto

2020

Chemistry & Biodiversity

View full text Add to dashboard Cite

Lamiaceae is one of the largest families of flowering plants comprising about 250 genera and over 7,000 species. Most of the plants of this family are aromatic and therefore important source of essential oils. Lamiaceae are widely used as culinary herbs and reported as medicinal plants in several folk traditions. In the Mediterranean area oregano, sage, rosemary, thyme and lavender stand out for geographical diffusion and variety of uses. The aim of this review is to provide recent data dealing with the phytochemical and pharmacological studies, and the more recent applications of the essential oils and the non‐volatile phytocomplexes. This literature survey suggests how the deeper understanding of biomolecular processes in the health and food sectors as per as pest control bioremediation of cultural heritage, or interaction with human microbiome, fields, leads to the rediscovery and new potential applications of well‐known plants.

show abstract

The effects of selected phytochemicals on biofilm formed by five methicillin-resistant Staphylococcus aureus

Cited by 38 publications

References 11 publications

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

New Tricks for Old Guys: Recent Developments in the Chemistry, Biochemistry, Applications and Exploitation of Selected Species from the Lamiaceae Family

Contact Info

Product

Resources

About