The effects of salts and amino group modification on the iron binding domains of transferrin

Allosteric effects of sulfonate anions on the rates of iron release from serum transferrin

“…The results are shown in Table 1. The acceleration of iron release from the C-terminal site by chloride and perchlorate agrees with several previous studies [27,38,39,[45][46][47][48]. The organic anions used in this study also accelerate iron release, but to a lesser degree compared with chloride and perchlorate.…”

“…A variety of simple inorganic anions alter the rates of iron release by various chelating agents [39,45,46,48,51,52]. These kinetic effects do not correlate well with simple changes in the ionic strength of the solutions [35].…”

Exploring the Fe(III) binding sites of human serum transferrin with EPR at 275 GHz

“…[29,[36][37][38] Binding with a different synergistic anion and mutation of the coordinating ligands alters the spectrum drastically, [39,31,[40][41][42][43] and also mutations further away from the iron-binding site are known to influence the spectrum, [44] as are salt concentration and pH. [45][46][47] Despite many attempts, a satisfactory interpretation of the X-band spectrum of transferrin has not yet been provided. [27,28,30,33,34,65,49] Variations in the spectra are neither understood nor quantified, as in most cases they are merely changes in signal intensity that reshape the spectra and not shifts in resonance fields, which would be more informative.…”

“…Discussion). [10,29,31,[35][36][37][38][39][40][41][42][43][44][45][46][47] A few EPR studies on transferrins at higher microwave frequencies exist. At 34 GHz extremely complex spectra were obtained.…”

Transferrin-Mediated Cellular Iron Delivery

“…In the past, many different spectroscopic techniques, including electron paramagnetic resonance spectroscopy (Chasteen, 1983; Folajtar & Chasteen, 1982; Grady, Mason, Woodworth, & Chasteen, 1995; Price & Gibson, 1972a; Thompson, McCarty, & Chasteen, 1986), nuclear magnetic resonance spectroscopy (Kubal, Mason, Patel, Sadler, & Woodworth, 1993) and UV-difference spectra (Harris, 1985; Harris & Bali, 1988; Harris, Cafferty, Abdollahi, & Trankler, 1998; Harris, Nesset-Tollefson, Stenback, & Mohamed-Hani, 1990; Pecoraro, Harris, Carrano, & Raymond, 1981) have been employed to detect anion binding to hTF. It was clearly demonstrated (Williams, Chasteen, & Moreton, 1982) that at pH 7.4, the presence of salt retards the rate of iron release from the N-lobe while facilitating iron release from the C-lobe.…”