The Effects of Salt Stress on Photosynthetic Electron Transport and Thylakoid Membrane Proteins in the Cyanobacterium Spirulina platensis

Sudhir, P.; Pogoryelov, Denys; Kovács, László; Garab, Győző; Murthy, S. D. S.

doi:10.5483/bmbrep.2005.38.4.481

Cited by 102 publications

(82 citation statements)

References 21 publications

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“…Similar findings were also reported by Seki et al (2002) and Chaves et al (2009). A decrease in carbon assimilation may also be attributed to the selective inhibition of photosystem II as reflected in NADPH production (Lu & Zhang, 2000;Srivastava et al, 2008;Sudhir et al, 2005). An enhanced oxygenase activity of Rubisco is therefore postulated due to a reduction in photosynthetic carbon assimilation that may decrease the amount of carbon in the carboxysome, exposing Rubisco to a higher oxygen partial pressure.…”

Section: Gene Encodingsupporting

confidence: 77%

Assessment of salinity-induced photorespiratory glycolate metabolism in Anabaena sp. PCC 7120

et al. 2011

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This paper reports an investigation of salinity-induced glycolate metabolism in the cyanobacterium Anabaena sp. PCC 7120 (hereafter Anabaena PCC 7120). Quantitative analysis of transcripts for the photosynthesis-associated genes encoding ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco), phosphoribulokinase and transketolase, as well as those involved in glycolate metabolism (phosphoglycolate phosphatase, glycolate oxidase, alanine-glyoxylate aminotransferase and serine hydroxymethyltransferase) was performed. The expression of all investigated photosynthesis-associated genes except Rubisco was downregulated after 24 h NaCl treatment. However, under the same conditions, the transcripts encoding enzymes involved in glycolate metabolism were overexpressed. This was further confirmed by the quantitative analysis of the intermediates involved in glycolate metabolism. The intracellular levels of organic acids (glyceric, glycolic and glyoxylic acids) and amino acids (glycine and serine) were elevated in salt-treated cells as compared to those in the control cells. Transcriptional inhibition of photosynthesis-associated genes, and upregulation of genes and enhanced synthesis of intermediates associated with glycolate metabolism, indicate the occurrence of this photorespiratory metabolic pathway metabolism in Anabaena PCC 7120 under salt stress. INTRODUCTIONThe evolution of cyanobacteria over 2.7 billion years significantly contributed to a major transition in the history of life on Earth (Buick, 1992). Cyanobacterial photosynthesis resulted in a tremendous increase in the oxygen concentration of the atmosphere and the emergence of oxygen-dependent life. Due to their ability to adapt frequently to changing environments, cyanobacteria currently occupy most ecological niches on our planet.An important habitat for cyanobacteria is rice fields, where they significantly contribute to the availability of nitrogen for the crop (Singh, 1961). The rice agro-ecosystem, including cyanobacteria, is subjected to several abiotic stresses, including salt stress caused by anthropogenic activities (Srivastava et al., 2009). It has been reported that high salinity not only inhibits photosynthesis and hence the carbon pool of freshwater cyanobacteria but also decreases the amount of fixed carbon available for the synthesis of compatible solutes (Ferjani et al., 2003). Responses of freshwater cyanobacteria to salt stress have been studied by several research groups at the genomic, transcriptomic and proteomic levels Huang et al., 2006;Kanesaki et al., 2002; Marin et al., 2004;Srivastava et al., 2008). These studies reported salt stress responses of several cyanobacterial species in relation to specific genes encoding stress proteins, photosynthetic proteins and proteins involved in the synthesis of compatible solutes. However, salinity-induced changes in metabolism related to glycolate metabolism (photorespiration) have been little studied in cyanobacteria.Photorespiration evolved as an essential disadvantage of oxygenic life, as...

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Section: Gene Encodingsupporting

confidence: 77%

Assessment of salinity-induced photorespiratory glycolate metabolism in Anabaena sp. PCC 7120

et al. 2011

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“…(Sudhir et al, 2005 ). Under salt stress, these ROS can cause damage to membranes and other essential macromolecules such as pigment, proteins, DNA, RNA, and lipids (Ashraf and Ali, 2008).…”

Section: +2mentioning

confidence: 99%

Impact of salt stress on photosystem II efficiency and antioxidant enzyme activities of safflower (Carthamus tinctorius L.) cultivars

Erdal¹,

Çakirlar²

2014

Turk J Biol

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This study was conducted to determine the tolerance of safflower (Carthamus tinctorius L.) cultivars (Dinçer, Remzibey-05, and Yenice) against salt stress based on some physiological and biochemical parameters at the vegetative stage. Eighteen-day-old plants were subjected to salt stress [0 (control), 75, 150, 225, and 300 mM NaCl concentrations] for 12 days, which led to a significant decrease in growth parameters (stem growth, fresh weight, and relative water content) and the photosynthetic pigment contents of the safflower cultivars. The photochemical activities of photosystem II of the cultivars were negatively affected by salinity, especially at the highest concentration (300 mM). Salt stress decreased K + content and K + /Na + ratio while it increased Na + content. Malondialdehyde and free proline contents in the leaves of cultivars increased gradually in proportion to the increase of NaCl concentration. Analysis of antioxidant enzyme activities showed that these enzymes responded differently to the NaCl concentrations. Dinçer, with higher antioxidant enzyme activities, had a more effective response than the other cultivars. Considering growth and biochemical and chlorophyll fluorescence parameters with the endogenous defense system, Dinçer had a higher withstanding capacity against salinity than the other cultivars.

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“…Vonshak et al (1996) demostraron que se produce una respuesta por parte del alga a un stress salino, las células se adaptan cambiando su composición bioquímica y reduciendo las proteínas y el contenido de clorofila. Estudios han demostrado que el estrés salino (0,8 M de NaCl) en A. platensis, inhibe la actividad del PSII (fotosistema II) de electrones estimulando la actividad del transporte de la PSI (fotosistema I), donde la actividad del PSII de células enteras se redujo hasta 60% durante las primeras 9 h del tratamiento, manteniéndose en ese nivel durante por lo menos 15 h. El estrés salino causó una pérdida del 40% en la eficiencia máxima del PSII y 54% de pérdida con la variable luminosidad (Sudhir et al, 2005).…”

Section: Discussionunclassified

“…platensis tiene la capacidad de crecer en condiciones de cultivo autótrofo (Shichi & Ogawa, 1977;Vieira et al, 2003;Batthacharya & Shivaprakash, 2005;Sudhir et al, 2005), mixotrófico y heterótrofo (Márquez et al, 1993;Chen et al, 1996;Zhang et al, 1999, Vonshak et al, 2000, Lodi et al, 2005Andrade & Costa, 2007;Dhiab et al, 2010).…”

Section: Introductionunclassified

Aplicacion de estrategias nutricionales y su efecto en el crecimiento en el cultivo continuo de Spirulina (Arthrospira platensis)

Rojas

Avila

Parada

2012

lajar

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Aplicación de estrategias nutricionales y su efecto en el crecimiento en el cultivo continuo de Spirulina (Arthrospira platensis) RESUMEN. Se realizó un cultivo continuo de Spirulina (Arthrospira platensis) durante 78 días en un fotobiorreactor tubular de 300 L de volumen. Durante este periodo se registró el crecimiento en biomasa y la respuesta del cultivo a la estrategia de adición del medio nutritivo. Utilizando como base el medio Zarrouk modificado, se elaboraron tres medios: medio inicial (MI), medio crecimiento (SA1+SA2) y medio mantenimiento (SB1+SB2) para las distintas etapas del cultivo. La entrega de los medios se efectuó paulatinamente y de acuerdo a los requerimientos del crecimiento en biomasa (en peso seco) diario del cultivo. La biomasa inicial del cultivo fue de 2,0 g L -1, al día 20 se efectuó la primera cosecha parcial (50%) dando inicio a una nueva etapa del cultivo con una biomasa inicial de 1,7 g L -1 , 20 días después se realizó una segunda cosecha parcial del cultivo, iniciando una tercera etapa de cultivo con una biomasa de 1,6 g L -1 . Los resultados obtenidos al cabo de las cosechas fueron, en promedio, de 3,7; 4,1 y 9,7 g L -1 , respectivamente. El análisis proximal efectuado al final del cultivo continuo indicó un 71,6% de proteína. Palabras clave: Arthrospira platensis, Spirulina, medio nutritivo, crecimiento, cultivo, Chile. Application of nutritional strategies and their effect in continuous culture of Spirulina (Arthrospira platensis)ABSTRACT. A continuous culture of Spirulina (Arthrospira platensis) was performed for 78 in a tubular photobioreactor of 300 L volume. During this period there was growth in biomass and crop response to the strategy of adding the nutrient medium. Using as a basis the modified Zarrouk medium, three nutritive combinations were elaborated: initial medium (MI), growth medium (SA1+SA2) and maintenance medium (SB1+SB2) for different crop stages. The media delivery is affected gradually and in accordance with requirements of growing biomass (dry weight) daily cultivation. The initial crop biomass was 2.0 g L -1, on day 20 of culture was made the first partial harvest (50%) starting a new stage of the crop with an initial biomass of 1.7 g L -1, 20 days after a second crop was partially culture initiating a third culture step with a biomass of 1.6 g L -1 . The results obtained after harvest were, on the average, 3.7; 4.1 and 9.7 g L -1 respectively. The proximal analysis was performed at the end of the continuous cultivation showed a 71.6% of protein content. Keywords: Arthrospira platensis, Spirulina, nutrient medium, growth, culture, Chile. ___________________Corresponding author: Elizabet Rojas (elizabet.rojas@cordunap.cl) INTRODUCCIÓNLa especie Arthrospira platensis, conocida como Spirulina, es una cianobacteria, capaz de convertir la energía del sol en compuestos químicos usando dióxido de carbono y liberando oxígeno. Por sus características, ha adquirido gran importancia en la industria de alimentos, farmacéutica y cosmética, debido a su alto valor nutri...

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The Effects of Salt Stress on Photosynthetic Electron Transport and Thylakoid Membrane Proteins in the Cyanobacterium Spirulina platensis

Cited by 102 publications

References 21 publications

Assessment of salinity-induced photorespiratory glycolate metabolism in Anabaena sp. PCC 7120

Assessment of salinity-induced photorespiratory glycolate metabolism in Anabaena sp. PCC 7120

Impact of salt stress on photosystem II efficiency and antioxidant enzyme activities of safflower (Carthamus tinctorius L.) cultivars

Aplicacion de estrategias nutricionales y su efecto en el crecimiento en el cultivo continuo de Spirulina (Arthrospira platensis)

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