The effects of rose bengal‐ and erythrosine‐mediated photodynamic therapy on Candida albicans

Abstract

The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using rose bengal or erythrosine with light emitting diode (LED) on Candida albicans planktonic cultures and biofilms. Seven C. albicans clinical strains and one standard strain (ATCC 18804) were used. Planktonic cultures and biofilms of each C. albicans strain were submitted to the following experimental conditions: (a) treatment with rose bengal and LED (RB+L+); (b) treatment with erythrosine and LED (E+L+); and (c) control group, without LED irradiation or photosensitiser treatment (P-L-). After irradiation of the planktonic cultures and biofilms, the cultures were seeded onto Sabouraud dextrose agar (37 °C at 48 h) for counting of colony-forming units (CFU ml(-1) ) followed by posterior anova and Tukey's test analyses (P < 0.05). The biofilms were analysed using scanning electron microscopy (SEM). The results revealed a significant reduction of planktonic cultures (3.45 log(10) and 1.97 log(10) ) and of biofilms (<1 log(10) ) for cultures that were subjected to PDT mediated using either erythrosine or rose bengal, respectively. The SEM data revealed that the PDT was effective in reducing and destroying of C. albicans blastoconidia and hyphae. The results show that erythrosine- and rose bengal-mediated PDT with LED irradiation is effective in treating C. albicans.

Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

“…The data presented in this study indicated that a 15-min application of erythrosine at the concentrations of 220 or 440 μM and stimulated by 420-480 nm blue LED light (15 J/cm 2 ) for 1 min significantly decreased C. albicans survival. These results are in accordance with a previous study by where erythrosine at 40 μM stimulated by the blue LED 95 J/cm 2 was shown to kill C. albicans [15]. We used the irradiation time of 1 min to mimic the practical time in a clinical setting.…”

Spray layer-by-layer films for photodynamic inactivation

“…The acridine orange photosensitizer could not only be 18 deposited on surface of C. albicans but also penetrate into the different cellular domains of 19 the microorganism such as the plasma membrane or mitochondria. The surface 20 morphological results are comparable to those reported by Costa et al [28]. Their scanning 21 tunneling microscopy (STM) images reveal that C. albicans cells were deformed after a PDI 22 procedure with erythrosine and LED light.…”

An in vitro study of the photodynamic effect of rose bengal on trichophyton rubrum

“…They are chosen on the basis of their charge, ability to be non-toxic without illumination (dark toxicity) and the ability to become genotoxic or mutagenic following irradiation [23]. Rose Bengal (RB) is a photosensitizer that has been found to be toxic to various microbes, including Streptococcus mutans and Candida albicans, when used with PDT [24,25]. It is an amphoteric dye that absorbs visible light between the wavelengths of 500 nm to 550 nm (encompassing the green light spectrum) [26] and its antimicrobial action is mostly mediated via the Type II photodynamic pathway [24].…”