The effects of probiotic Bacillus coagulans on the cytotoxicity and expression of alpha toxin gene of Clostridium perfringens type A

Kawarizadeh, Amin; Tabatabaei, Mohammad; Hosseinzadeh, Saeid; Farzaneh, Mina; Pourmontaseri, Maryam

doi:10.1016/j.anaerobe.2019.05.008

Cited by 15 publications

(14 citation statements)

References 33 publications

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“…For example, the Bacillus licheniformis bacteriocin called Bacillusin 490 that produce at 4°C and at a wide range of pH has antimicrobial activities against pathogens such as Bacillus smithii, Bacillus anthracis, Bacillus cereus and Listeria innocua (30). Previous studies have also shown that extracts obtained from probiotic B. subtilis and B. coagulans cultures showed an inhibitory effect on the Clostridium perfringens (17, 31). In the present study, the MIC of supernatant obtained from the culture of B. coagulans with concentration of 8% showed the highest inhibitory effect on the growth of S .…”

Section: Discussionmentioning

confidence: 94%

The effect of Bacillus coagulans on cytotoxicity and apoptosis induced by Salmonella Typhimurium in HT-29 cell culture

Kawarizadeh

Nojoomi

Tabatabaei

et al. 2019

IJM

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Background and Objectives: Human epithelial cells have been widely used to study the interaction between intestinal cells and pathogens, in vitro. In this study, the effect of probiotic bacteria Bacillus coagulans and its supernatant on the growth inhibition, cytotoxicity and induction of apoptosis caused by Salmonella Typhimurium and its adhesion to HT-29 cells were investigated. Materials and Methods: B. coagulans supernatant was used to obtain the minimum inhibitory concentration. To evaluate the cytotoxicity and percent of apoptotic cells, B. coagulans and its supernatant (2, 4, 6 and 8% concentrations) with S. Typhimurium was added to HT-29 cells. The MTT assay was used in order to evaluate the cytotoxicity. Percent of apoptotic cells was reported using a fluorescence staining method. Additionally, the adhesion of S. Typhimurium to HT-29 cells was investigated. The effect of B. coagulans on the level of adhesion was also studied. Results: The most inhibitory effect was shown at the concentration of 80000 µg/ml supernatant of B. coagulans (54.77% ± 1.43). The simultaneous culture of S. Typhimurium with B. coagulans had the lowest amount of cytotoxicity and induced apoptosis among the all co-culture groups of S. Typhimurium with B. coagulans or its supernatant. The determined cytotoxicity and induced apoptosis were 26.06 % ± 3.79 and 17.63 % ± 2.14 respectively. In the adhesion test, it was observed that B. coagulans can significantly prevent adhesion of S. Typhimurium to HT-29 cell. Conclusion: B. coagulans can reduce the adhesion, cytotoxicity and induction of apoptosis caused by S. Typhimurium in HT-29 cells in vitro.

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Section: Discussionmentioning

confidence: 94%

The effect of Bacillus coagulans on cytotoxicity and apoptosis induced by Salmonella Typhimurium in HT-29 cell culture

Kawarizadeh

Nojoomi

Tabatabaei

et al. 2019

IJM

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“…However, B. amyloliquefaciens can produce gas by utilizing the residual sugar (Meng et al., 2019). C. perfringens is a gram‐positive spore‐producing obligate anaerobic bacterium that widely exists in natural soil, sewage, feces, and human and animal intestinal tracts (Kawarizadeh, Tab atabaei, Hosseinzadeh, Farzaneh, & Pourmontaseri, 2019; Uzal et al., 2012; Xiao, Wagendorp, Abee, & Wills‐Bennik, 2015). The genome of C. perfringens contains typical anaerobic fermentation enzymes that lead to gas production but does not contain enzymes for tricarboxylic acid cycle or respiratory chain (Shimizu et al., 2002).…”

Section: Resultsmentioning

confidence: 99%

Analysis and control of microbial gas production in fermented chili paste

Zheng

Chen

et al. 2020

J. Food Process. Preserv.

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“…2017; Kawarizadeh et al . 2019b) which was measured by the Bradford method (Bradford 1976; Poormontaseri et al . 2017; Kawarizadeh et al .…”

Section: Methodsmentioning

confidence: 99%

“…2011; Kawarizadeh et al . 2019b). Percentage inhibition of bacterial growth was determined using the following equation: [(OD positive control (between 0 and 24 h)−OD sample (between 0 and 24 h))/OD positive control (between 0 and 24 h) × 100].…”

Section: Methodsmentioning

confidence: 99%

“…Cell maintenance and culture were carried out as described previously (Kawarizadeh et al . 2019b). Briefly, the cells were cultured in Roswell Park Memorial Institute medium (RPMI 1640) (Shell Max, Iran) with 10% inactivated fetal bovine serum (Gibco Inc., Grand island, NY, USA), penicillin (100 U ml −1 ) and streptomycin (100 µg ml −1 ) (Bio‐idea Inc., Tehran, Iran).…”

Section: Methodsmentioning

confidence: 99%

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Interleukin‐8 gene expression and apoptosis induced by Salmonella Typhimurium in the presence of Bacillus probiotics in the epithelial cell

Kawarizadeh

Pourmontaseri

Farzaneh

et al. 2020

J. Appl. Microbiol.

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Aims This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin‐8 (IL‐8) gene expression in the co‐culture of the Bacillus and the pathogen in vitro. Methods and Results Bacillus subtilis, Bacillus indicus and Bacillus coagulans were initially turned to spore and heat‐inactivated forms. The cellular damages of the probiotics on the HT‐29 cells were investigated individually and in combination with S. Typhimurium using 3‐(4,5 dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) and fluorescence assays. To extract cell free supernatants (CFS) of the probiotics, they were cultured in selective media. The inhibitory activity of CFSs were then assayed against the pathogen. The gene expression of IL‐8 of the HT‐29 cells was evaluated by real‐time PCR in all the groups. The results showed that the CFSs of three probiotics could inhibit the growth of S. Typhimurium by more than 50%. Inhibitory effects of B. indicus and B. subtilis CFSs were related to the production of pepsin‐sensitive compounds, except B. coagulans in which the high inhibitory effect was due to organic acids. The spores of the three probiotics and the heat‐inactivated forms of B. subtilis and B. coagulans could reduce the cytotoxicity of S. Typhimurium. The cell viability also increased applying both forms probiotics against the pathogen. In all co‐culture groups, the IL‐8 gene expression induced by S. Typhimurium was reduced. Conclusions The three Bacillus probiotics can be considered as proper candidates for the prevention and treatment of S. Typhimurium food poisoning. Significance and Impact of the Study Applying probiotics as live bacteria is universally noted in foods. This study tried to discover the effects of Bacillus probiotics in the form of spore or even heat‐killed bacteria against S. Typhimurium and evaluate ratio of IL‐8 gene expression in cell culture. The most effective Bacillus probiotic will be recommended. This approach will help to use probiotics as nonvegetative cells in foods to fight gastrointestinal pathogens.

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The effects of probiotic Bacillus coagulans on the cytotoxicity and expression of alpha toxin gene of Clostridium perfringens type A

Cited by 15 publications

References 33 publications

The effect of Bacillus coagulans on cytotoxicity and apoptosis induced by Salmonella Typhimurium in HT-29 cell culture

The effect of Bacillus coagulans on cytotoxicity and apoptosis induced by Salmonella Typhimurium in HT-29 cell culture

Analysis and control of microbial gas production in fermented chili paste

Interleukin‐8 gene expression and apoptosis induced by Salmonella Typhimurium in the presence of Bacillus probiotics in the epithelial cell

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