The Effects of Preconditioning on Survival of Mesenchymal Stem Cells in Vitro

Taban, Zahra Farzaneh; Khatibi, Shiva; Halabian, Raheleh; Roushandeh, Amaneh Mohammadi

doi:10.17795/gct-40229

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…Taban et al in a report consistent with our results demonstrated that co- preconditioning of MSCs with low serum and H 2 O 2 has prevented cell death induced by 300 µM H 2 O 2 . 23 McAdle et al reported that the pretreatment of cultured skeletal muscle myotubes with a non-damaging concentration of H 2 O 2 caused significant protection against damaging treatment of H 2 O 2 . 24…”

Section: Resultsmentioning

confidence: 99%

Preconditioning of Mesenchymal Stem Cells with Non-ToxicConcentration of Hydrogen Peroxide Against Oxidative StressInduced Cell Death: The Role of Hypoxia-Inducible Factor-1

2019

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Purpose: To investigate the protective effect of preconditioning with non-toxic dose of hydrogenperoxide (H2O2) as a possible cell signaling molecule, against cell death induced by toxicconcentration of H2O2 or by serum deprivation in human Wharton’s jelly-derived mesenchymalstem cells (HWJ-MSCs) and underlying mechanisms.Methods: HWJ-MSCs were isolated and identified using flow cytometry. After finding non-toxicconcentration of H2O2, cells preconditioning was performed by H2O2 (20 μM) for 12 h and celltolerance against serum deprivation or toxic levels of H2O2 was assayed by MTT test. Effect ofpreconditioning on mRNA and protein expression of Akt-1, Bcl-2 and Bax were examined usingreverse transcription polymerase chain reaction (RT-PCR) and western blotting respectively. Roleof hypoxia-inducible factor (HIF)-1α was explored in presence HIF-1α inhibitor.Results: Preconditioning with 20 μM H2O2 for 12 h was non-toxic and decreased cell deathinduced by oxidative stress and serum deprivation in MSC cultures. However, the increasedtolerance reversed in the presence of inhibitor of HIF-1α. By regards to RT-PCR and westernblotting data, although expression of Akt-1, Bcl-2 and Bax was not change considerably butphosphorylated Akt-1 (pAkt-1) was up regulated after treatment with 20 μM H2O2 compared tocontrol group. Moreover after exposure to 100 μM H2O2, western blotting analysis showed thatcell pretreatment with 20 μM H2O2, decremented Bax/Bcl2 ratio and up-regulated HIF-1α andpAkt-1 compared to the control group.Conclusion: Increased tolerance of H2O2-pretreated cells led to the suggestion that transplantationof H2O2 preconditioned MSCs may improve therapeutic potential of stem cells in cell therapyprocedures.

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Section: Resultsmentioning

confidence: 99%

Preconditioning of Mesenchymal Stem Cells with Non-ToxicConcentration of Hydrogen Peroxide Against Oxidative StressInduced Cell Death: The Role of Hypoxia-Inducible Factor-1

2019

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“…Thus, researchers had tried to develop new strategies to improve survival of MSCs, such as preconditioning of MSCs by cultivation under peroxide hydrogen, hypoxic and serum deprivation (Farzaneh Taban et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells

et al. 2016

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Human Gingival Mesenchymal Stem Cells (hGMSCs) are multipotential cells that can expand and differentiate in culture under specific and standardized conditions. In the present study, we have investigated whether in vitro pre-treatment of hGMSCs with Cannabidiol (CBD) can influence their expression profile, improving the therapeutic potential of this cell culture. Following CBD treatment (5 μM) for 24 h, gene expression analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed between CBD-treated hGMSCs (CBD-hGMSCs) and control cells (CTR-hGMSCs) that were linked to inflammation and apoptosis. In particular, we have demonstrated that CBD treatment in hGMSCs prevented the activation of the NALP3-inflammasome pathway by suppressing the levels of NALP3, CASP1, and IL18, and in parallel, inhibited apoptosis, as demonstrated by the suppression of Bax. CBD treatment was also able to modulate the expression of the well-known mesenchymal stem cell markers (CD13, CD29, CD73, CD44, CD90, and CD166), and other surface antigens. Specifically, CBD led to the downregulation of genes codifying for antigens involved in the activation of the immune system (CD109, CD151, CD40, CD46, CD59, CD68, CD81, CD82, CD99), while it led to the upregulation of those implicated in the inhibition of the immune responses (CD47, CD55, CD276). In conclusion, the present study will provide a new simple and reproducible method for preconditioning hGMSCs with CBD, before transplantation, as an interesting strategy for improving the hGMSCs molecular phenotype, reducing the risk of immune or inflammatory reactions in the host, and in parallel, for increasing their survival and thus, their long-term therapeutic efficacy.

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“…This makes a requirement to ponder an approach to refine the efficacy MSCs and improve the survival rate (Spees, Lee, & Gregory, 2016;van Velthoven, Kavelaars, & Heijnen, 2012). In this regard, preconditioning or pretreatment of MSCs with sublethal environmental stress adjusts them to destructive conditions then protects cells from apoptosis and results in a better survival rate (Liu, Wang, Ji, Yu, & Wei, 2014;Saparov, Ogay, Nurgozhin, Jumabay, & Chen, 2016;Taban, Khatibi, Halabian, & Roushandeh, 2016). Latest data show that endotoxin preconditioning could protect MSCs against apoptosis induced by hydrogen peroxide (H 2 O 2 ) or/and serum deprivation, at the same time as increasing the in vitro viability of MSCs (Amiri, Jahanian-Najafabadi, & Roudkenar, 2015;Zhou et al, 2015).…”

mentioning

confidence: 99%

Antimicrobial effects of mesenchymal stem cells primed by modified LPS on bacterial clearance in sepsis

Saeedi

Halabian

Fooladi

2018

Journal Cellular Physiology

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The Effects of Preconditioning on Survival of Mesenchymal Stem Cells in Vitro

Cited by 5 publications

References 20 publications

Preconditioning of Mesenchymal Stem Cells with Non-ToxicConcentration of Hydrogen Peroxide Against Oxidative StressInduced Cell Death: The Role of Hypoxia-Inducible Factor-1

Preconditioning of Mesenchymal Stem Cells with Non-ToxicConcentration of Hydrogen Peroxide Against Oxidative StressInduced Cell Death: The Role of Hypoxia-Inducible Factor-1

Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells

Antimicrobial effects of mesenchymal stem cells primed by modified LPS on bacterial clearance in sepsis

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