The Effects of Platelet-Rich-Plasma Gel Application to the colonic anastomosis in Hyperthermic intraperitoneal chemotherapy: An Experimental Rat Model

OCAK, SÖNMEZ; Bük, Ömer Faruk; Genç, Buğra; Avcı, Bahattin; Uzuner, Hatice Ölger; Gündoğdu, Salih Burak

doi:10.31923/pooltext-preprint-1062-4329-0098

Cited by 2 publications

(5 citation statements)

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“…These results show that the HIPEC procedure decreases tissue hydroxyproline levels in colon anastomosis, but this situation can be improved by the PRP gel application that increases these levels. In our study, significantly lower tissue hydroxyproline levels were detected in the 5‐FU HIPEC groups, which is consistent with the literature 10,24 …”

Section: Discussionsupporting

confidence: 93%

“…There was also a statistically significant difference between the 5‐FU group and the 5‐FU + PRP group ( P = 0.047), which indicates that PRP significantly increases ABP. These findings suggest that the negative effect of HIPEC on anastomotic healing can be reversed by the application of PRP gel, which is supported by similar studies in the literature 13,23,24,27 …”

Section: Discussionsupporting

confidence: 81%

“…In our study, significantly lower tissue hydroxyproline levels were detected in the 5-FU HIPEC groups, which is consistent with the literature. 10,24 The measurement of ABP is a reliable method for assessing early post-operative anastomotic mechanical strength, especially within *The difference between groups 2 and 3 is significant (P = 0.020). **The difference between groups 2 and 3 is significant (P = 0.015).…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that the negative effect of HIPEC on anastomotic healing can be reversed by the application of PRP gel, which is supported by similar studies in the literature. 13,23,24,27 Since the 1970s, the positive effects of administration of autologous PRP gel obtained from the patients' own blood have been shown to improve healing in chronic wounds, such as diabetic foot ulcers and venous ulcers. Due to the advantage of preparing the treatment material from the patients' own blood, some researchers referred to PRP as 'key for self-regeneration'.…”

Section: Discussionmentioning

confidence: 99%

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Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

Göksu

Alakuş

Ertan

et al. 2020

ANZ Journal of Surgery

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Background: Hyperthermic intra-peritoneal chemotherapy (HIPEC) performed in patients with peritoneal carcinomatosis has a cytotoxic effect on tumour cells. This cytotoxic effect also adversely affects the wound healing of anastomosis, which is frequently undertaken in this patient group, and causes anastomotic leakage and intestinal fistulas. Platelet-rich plasma (PRP) gel has been used in wound healing and has shown efficacy. We investigated the effect of PRP gel on colon anastomosis healing in rats treated with 5-fluorouracil (5-FU). Methods: Twenty-four rats were divided into three groups, each containing eight rats: group 1 (control) underwent colon anastomosis alone; group 2 (5-FU) underwent a colon anastomosis and HIPEC; and group 3 (5-FU + PRP) underwent a colon anastomosis and HIPEC and received topical PRP gel. The rats were sacrificed on the post-operative day 7 and the anastomotic bursting pressure (ABP) was measured. Tissue samples were obtained to measure the hydroxyproline level and investigate the histopathological changes on the anastomosis line. Results: ABP was higher in the control and 5-FU + PRP groups than in the 5-FU group (P = 0.009, P = 0.047). The tissue hydroxyproline levels were higher in the control and 5-FU + PRP groups than in the 5-FU group (P = 0.001, P = 0.030). The histopathological findings of the 5-FU + PRP group were better than those of the 5-FU group. Conclusion: This study showed that the application of PRP gel to colon anastomoses increased ABP in rats treated with HIPEC using 5-FU. It also showed positive effects on anastomotic healing by increasing the tissue hydroxyproline levels on the anastomosis line and reducing the inflammatory response.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

Göksu

Alakuş

Ertan

et al. 2020

ANZ Journal of Surgery

View full text Add to dashboard Cite

show abstract

“…Our results encouraged us to design a novel experimental model. Recently, its anastomotic healing effect has been evaluated by two similar studies regarding intraperitoneal administration of oxaliplatin and cisplatin with HIPEC 17 , 18 . However, 5-FU is commonly used for early EPIC 1 .…”

Section: Introductionmentioning

confidence: 99%

The influence of platelet-rich plasma (PRP) on colonic anastomosis healing impaired by intraperitoneal 5-flourouracil application. An experimental study

et al. 2020

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Purpose 5-flourourasil (5-FU) is commonly used for early intraperitoneal chemotherapy in colorectal or appendiceal cancer patients with peritoneal carcinomatosis. Due to its effect, anastomosis healing can be impaired and leads to anastomotic leakage. In this study, we aimed to investigate the potential healing effect of platelet-rich plasma (PRP) on colonic anastomosis impaired by intraperitoneal 5-flourouracil application. Methods After ten rats were sacrificed for preparing PRP, forty Wistar-albino rats were subjected to colonic anastomosis, and randomly allocated into four groups including 10 rats each. According to receiving PRP and/or 5-FU application, the groups were formed as control (C), 5-FU without PRP (CT), anastomosis with PRP (C-PRP), and 5-FU with PRP (CT-PRP). CT and CT-PRP groups also received 5-FU intraperitoneally on postoperative day 1 (POD 1). All animals were euthanized on pod 7. The body weight change, anastomotic bursting pressure (ABP), tissue hydroxiprolin (TH) and histopathological examination of each group were analyzed. Results 5-FU application significantly reduced ABP levels when compared with group C, C-PRP and CT-PRP (for each comparison, p<0,01). PRP application in CT-PRP group raised the measure of ABP up to the levels of C group. Although tissue hydroxyproline levels (THL) levels of CT-PRP group were found higher than CT group, it was not significant (p=0.112). Microscopically, comparing with CT group, PRP application significantly promoted the healing of colonic anastomosis subjected to 5-FU application by improving tissue edema, necrosis, submucosal bridging and collagen formation (p<0.05). Tissue healing in CT-PRP group was observed as good as the control groups. (C, C-PRP, p=0.181, p=0.134; respectively). Conclusion PRP administration on colonic anastomosis significantly promotes the healing process of anastomosis in rats receiving 5-FU. This result encourages further clinical use of PRP to reduce the frequency of AL in patients receiving EPIC.

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The Effects of Platelet-Rich-Plasma Gel Application to the colonic anastomosis in Hyperthermic intraperitoneal chemotherapy: An Experimental Rat Model

Cited by 2 publications

References 0 publications

Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

The influence of platelet-rich plasma (PRP) on colonic anastomosis healing impaired by intraperitoneal 5-flourouracil application. An experimental study

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