The Effects of Platelet-Derived Growth Factor-BB on Healing of the                     Rabbit Medial Collateral Ligament

Hildebrand, Kevin A.; Woo, Savio L‐Y.; Smith, David W.; Allen, Christina R.; Deie, Masataka; Taylor, Brian J.; Schmidt, Christopher C.

doi:10.1177/03635465980260041401

Cited by 263 publications

(164 citation statements)

References 25 publications

Supporting

Mentioning

154

Contrasting

Unclassified

Order By: Relevance

“…The highest dose in our study was three times less than the dose previously reported in studies investigating the effects of PDGF-BB on soft tissue healing [16,20]. A recent study investigating growth factor expression profile after rotator cuff surgery in a rabbit model noted that PDGF-BB expression was most prominent during Days 7 through 14 postsurgery [18].…”

Section: Discussioncontrasting

confidence: 54%

“…After medial collateral ligament rupture in rabbits, 20 lg of PDGF-BB with fibrin sealant delivered on the ligamentous surface demonstrated greater cellularity and vascularity, increased cross-sectional area, and greater ultimate load and energy absorbed to failure at 6 weeks postsurgery compared with the fibrin sealant group [16]. In a rat patellar tendon-defect model, a single bolus injection of 1 lg PDGF into the wound site led to greater cellular proliferation at 2 weeks for early supplementation and increased peak load, cross-sectional area, and pyridinoline content for later supplementation [5].…”

Section: Discussionmentioning

confidence: 93%

“…PDGF-BB has been found to act as a mitogen and chemotactic cytokine that can potentially enhance ligament and tendon healing in small animal models [5,16,22]. In a rat model of rotator cuff repair, delivery of cells expressing PDGF-BB with a polyglycolic acid scaffold showed restoration of normal crimp patterning and collagen bundle alignment compared with suture repair only [28].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

rhPDGF-BB Promotes Early Healing in a Rat Rotator Cuff Repair Model

Kovacevic

Gulotta

Liang

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Tendon-bone healing after rotator cuff repair occurs by fibrovascular scar tissue formation, which is weaker than a normal tendon-bone insertion site. Growth factors play a role in tissue formation and have the potential to augment soft tissue healing in the perioperative period. Questions/purposes Our study aim was to determine if rhPDGF-BB delivery on a collagen scaffold can improve tendon-to-bone healing after supraspinatus tendon repair compared with no growth factor in rats as measured by (1) gross observations; (2) histologic analysis; and (3) biomechanical testing. Methods Ninety-five male Sprague-Dawley rats underwent acute repair of the supraspinatus tendon. Rats were randomized into one of five groups: control (ie, repair only), scaffold only, and three different platelet-derived growth factor (PDGF) doses on the collagen scaffold. Animals were euthanized 5 days after surgery to assess cellular proliferation and angiogenesis. The remaining animals were analyzed at 4 weeks to assess repair site integrity by gross visualization, fibrocartilage formation with safranin-O staining, and collagen fiber organization with picrosirius red staining, and to determine the biomechanical properties (ie, load-to-failure testing) of the supraspinatus tendon-bone construct. Results The repaired supraspinatus tendon was in continuity with the bone in all animals. At 5 days, rhPDGF-BB delivery on a scaffold demonstrated a dose-dependent response in cellular proliferation and angiogenesis compared with the control and scaffold groups. At 28 days, with the numbers available, rhPDGF-BB had no effect on increasing fibrocartilage formation or improving collagen fiber maturity at the tendon-bone insertion site compared with controls. The control group had higher tensile loads to failure and stiffness (35.5 ± 8.8 N and 20.3 ± 4.5 N/mm) than all the groups receiving the scaffold, including the PDGF groups (scaffold: 27 ± 6.4 N, p = 0.021 and 13 ± 5.7 N/mm, p = 0.01; 30 lg/mL PDGF: 26.5 ± 7.5 N, p = 0.014 and 13.3 ± 3.2 N/mm, p = 0.01; 100 lg/mL PDGF: 25.7 ± 6

show abstract

Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

rhPDGF-BB Promotes Early Healing in a Rat Rotator Cuff Repair Model

Kovacevic

Gulotta

Liang

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…From a biomechanics perspective, rabbit medial collateral ligament scars fail at measures between 30 and 50% of normal values. [2][3][4] Even at subfailure, or low loads, scar has more creep (stretches out more) than normal ligament. 5 Biochemically, water content, collagen amounts and types, and other matrix components are altered.…”

Section: Clinical Problemsmentioning

confidence: 99%

Gene intervention in ligament and tendon: current status, challenges, future directions

Hildebrand

2004

Gene Ther

View full text Add to dashboard Cite

“…This is an additional limitation of this study. Regarding PDGF from main platelet alpha-granule content, Hildebrand et al (1998) reported that the application of PDGF significantly enhanced healing of the medial collateral ligament injury in a rabbit model. Recently, however, Nagumo et al (2005) reported that PDGF has no effect on the mechanical deterioration of in situ frozen ACL.…”

Section: Discussionmentioning

confidence: 99%

Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Yasuda

Sakai

Kondo

et al. 2007

Clinical Biomechanics

View full text Add to dashboard Cite

Introduction. The purpose of this study is to biomechanically and histologically evaluate the effect of bleeding from bone marrow on remodeling of the in situ frozen-thawed anterior cruciate ligament.Methods. Forty-four rabbits were used. Eight rabbits were used to evaluate the amount of bleeding at days 1-4. Thirty-six rabbits were divided into 2 groups, after the right anterior cruciate ligament underwent the freeze-thaw treatment. In Group I, no treatments were applied. In Group II, a tunnel was drilled into the bone marrow the femoral intercondylar notch. Each rabbit was sacrificed at 6 or 12 weeks. We examined the mechanical properties, and the histology of the anterior cruciate ligament. Results. A small amount of blood clot was observed only on the first day inGroup I, while a large amount of blood clot was seen around the anterior cruciate ligament for 3 days after surgery in Group II. In the midsubstance, a number of cells were scattered in Group II, no cells were seen in Group I at 6 weeks. The tangent modulus showed some tendency of difference between Groups I and II at each period.Discussion. Bleeding from the bone marrow obviously enhanced extrinsic cell infiltration into the in situ frozen-thawed anterior cruciate ligament at 6 weeks, and 2 showed some effects on its mechanical properties. This study implied that, in anterior cruciate ligament reconstruction, the blood from a bone tunnel play an important role in graft remodeling.3

show abstract

The Effects of Platelet-Derived Growth Factor-BB on Healing of the Rabbit Medial Collateral Ligament

Cited by 263 publications

References 25 publications

rhPDGF-BB Promotes Early Healing in a Rat Rotator Cuff Repair Model

rhPDGF-BB Promotes Early Healing in a Rat Rotator Cuff Repair Model

Gene intervention in ligament and tendon: current status, challenges, future directions

Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Contact Info

Product

Resources

About