The Effects of Plant Growth Substances on in Vitro Fiber Development From Fertilized Cotton Ovules

Beasley, C. A.; Ting, Irwin P.

doi:10.1002/j.1537-2197.1973.tb10209.x

Cited by 184 publications

(127 citation statements)

References 15 publications

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“…Seeds of Gossypium hirsutum L. ('Acala SJ-1') were obtained from Hubert Cooper, Jr., USDA/ARS U.S. Cotton Research Station, Shafter, Calif. They were germinated and grown as described by Beasley and Ting (9) with the exception that plants were maintained in growth chambers under the following light and temperature regime: 11.5 hr light (10 hr, incandescent and fluorescent lamps, followed by 1.5 hr from incandescent lamps only) at 33 C; 12.5 hr dark at 22 C. Plantings were made at 3-week intervals and the plants were discarded after 4 months. On the morning of anthesis, flowers were labeled with tags and were fertilized by gently brushing the anthers to dislodge pollen.…”

Section: Methodsmentioning

confidence: 99%

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Changes in Biochemical Composition of the Cell Wall of the Cotton Fiber During Development

Meinert¹,

Delmer²

1977

Plant Physiol.

296

199

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The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) has been studied from the early stages of elongation (5 days postaathesis) through the period of secondary wall formation, using cell wals derived both from fibers developing on the plant and from fibers obtained from excised, cultured ovules. The cell wall of the elongating cotton fiber was shown to be a dynamic structure.

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Section: Methodsmentioning

confidence: 99%

“…Unfertilized ovules were taken on the day of anthesis and cultured as described by Beasley and Ting (9,10 (43). Following methylation, 1 ml of chloroform-methanol (1:1, v/v) was added and this solution dialyzed for 2 days against four changes of 2 liters each of distilled H20.…”

Section: Methodsmentioning

confidence: 99%

Changes in Biochemical Composition of the Cell Wall of the Cotton Fiber During Development

Meinert¹,

Delmer²

1977

Plant Physiol.

296

199

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“…Very rapid growth of the corolla occurs during the 2 d just before anthesis. Likewise, evidence has been reported that IAA stimulates elongation ofthe epidermal cells on seed coats that become cotton fibers (4,20). The maximum rate of fiber elongation occurs between 8 and 10 d after anthesis (20), a time when the free and ester IAA contents of bolls are high (Table I).…”

Section: Resultsmentioning

confidence: 93%

Changes in Amide-Linked and Ester Indole-3-Acetic Acid in Cotton Fruiting Forms during Their Development

Guinn¹,

Brummett²

1989

Plant Physiol.

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The concentration of free indoleacetic acid (IAA) is high in cotton (Gossypium hirsutum L.) fruiting forms before anthesis, but is low at and for a few days after anthesis. Amide-linked and ester IAA were measured in fruiting forms at 9, 6, and 3 days before anthesis; at anthesis; and at 2, 4, 7, and 9 days after anthesis to determine if free IAA decreased because it was converted to a conjugated form. Cotton (Gossypium hirsutum L.) fruiting forms rarely abscise during the week before anthesis (12, 22), but are most susceptible to abscission during the week after anthesis (16,22). Abscission rate then decreases with increasing fruit (boll) age until it is essentially zero at 18 d after anthesis (15,16).Because exogenous IAA delays or prevents abscission (2, 7), we measured the concentration of free IAA in fruiting forms from 9 d before until 9 d after anthesis (18). The concentration of free IAA decreased about 80% during the 6 d before anthesis, remained low for the next 4 d, and then increased rapidly between 7 and 9 d after anthesis. Abscission rate increased rapidly after anthesis to a maximum at 5 and 6 d postanthesis. it then decreased as free IAA increased. Because these changes in free IAA were inversely correlated with changes in fruiting-form abscission, we suggested that the high concentration of IAA in large flower buds inhibited abscission before anthesis, whereas the low concentration at and for 4 d after anthesis promoted abscission of young bolls (18).A decrease in the concentration of free IAA could result from decreased synthesis, increased destruction, export, conversion to another form, or a combination of these possible events. We measured the concentrations of ester and amidelinked IAA before, at, and after anthesis to see if the decrease in free IAA was associated with a similar increase in either of the conjugated forms. MATERIALS AND METHODS Plant MaterialCotton (Gossypium hirsutum L.), cv Deltapine 61,' was grown in a field at the Western Cotton Research Laboratory in Phoenix as reported earlier (18). Flower buds were harvested 9, 6, and 3 before anthesis; flowers were harvested at anthesis; and bolls were harvested 2, 4, 7, and 9 d after anthesis. All fruiting forms (buds, flowers, and bolls) were harvested the same day to minimize possible differences due to any change in environment. The fruiting forms were rinsed in cold water, quickly frozen at -80°C, and lyophilized. The dry tissue was ground to pass a 40-mesh screen and stored under N2 at -80°C.Analyses of Ester and Amide IAA Samples of 200 or 500 mg each were extracted overnight in 70% acetone that contained antioxidants (19). '4C-IAA was added as an internal standard. The samples plus three 10-mL rinses with 70% acetone were filtered and the volume reduced to about 9 mL by rotary evaporation at 45°C. Ester IAA was hydrolyzed by adding 1 ml of 10 N KOH, mixing, and incubating 1 h at room temperature (3). Amide-linked IAA was hydrolyzed in 7 N NaOH during 3 h at about 100°C (3). All samples were hydrolyzed in a nitrogen a...

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“…Developing ovules were carefully dissected from the ovaries under sterile conditions and immediately floated on the liquid medium containing 5 μmol/L indole-3-acetic acid (IAA) and 0.5 μmol/L gibberellic acid (GA 3 ) in a 100-ml flask (Beasley and Ting, 1973). The ovules were kept at 30 °C in the dark.…”

Section: Ovule Culture Using Respiratory Inhibitorsmentioning

confidence: 99%

“…The cotton ovule culture technique was established four decades ago and has been applied in research related to cell biology, biochemistry, and the molecular biology of fiber development (Beasley and Ting, 1973;Beasley et al, 1974;Wang et al, 2002;Sun et al, 2005;Shi et al, 2006;Taliercio and Haigler, 2011). There are no differences between cultured ovule and field-grown plant fibers, with respect to morphological and biochemical characters, specifically fiber elongation and wall thickening (Beasley et al, 1974;Meinert and Delmer, 1977;Carpita and Delmer, 1981).…”

Section: Introductionmentioning

confidence: 99%

In vitro inhibition of pigmentation and fiber development in colored cotton

Yuan

Malik

Hua

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:Colored cotton has naturally pigmented fibers. The mechanism of pigmentation in cotton fiber is not well documented. This experiment was conducted to study the effects of respiratory chain inhibitors, i.e., rotenone and thiourea, on pigmentation and fiber development in colored cotton. After 1 d post-anthesis, ovaries were harvested and developing ovules were cultured on the liquid medium containing different concentrations of rotenone and thiourea for 30 d. The results demonstrate that both respiratory inhibitors reduced fiber length and ovule development under ovule culture conditions, and the inhibition efficiency of rotenone was much higher than that of thiourea. Rotenone and thiourea also showed significant effects on fiber pigment (color) development in colored cotton. In green cotton fiber, rotenone advanced fiber pigment development by 7 d at 200 μmol/L, while thiourea inhibited fiber pigmentation at all treatment levels (400, 600, 800, 1 000, and 2 000 μmol/L). Both respiratory inhibitors, however, had no significant effects on pigmentation of brown cotton fibers. The activities of cytochrome c oxidase (COX) and polyphenol oxidase (PPO) decreased significantly with increasing levels of both respiratory inhibitors. It is suggested that both respiratory inhibitors have important roles in deciphering the mechanism of pigmentation and fiber development in colored cotton.

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The Effects of Plant Growth Substances on in Vitro Fiber Development From Fertilized Cotton Ovules

Cited by 184 publications

References 15 publications

Changes in Biochemical Composition of the Cell Wall of the Cotton Fiber During Development

Changes in Biochemical Composition of the Cell Wall of the Cotton Fiber During Development

Changes in Amide-Linked and Ester Indole-3-Acetic Acid in Cotton Fruiting Forms during Their Development

In vitro inhibition of pigmentation and fiber development in colored cotton

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