The effects of pH level and calcium carbonate on biomass and mineral uptake of blueberry grown in tissue-culture medium

Tamir, Guy; Bar‐Tal, Asher; Zilkha, S.; Rotbaum, A.; Oren, Adi; Freund, G.; Dai, Nir

doi:10.17660/actahortic.2019.1265.28

Cited by 6 publications

(7 citation statements)

References 22 publications

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“…Therefore, based on the results of the present study and of our previous study (Tamir et al 2019), we suggest that rabbiteye blueberry root development is retarded or partially dysfunctional under neutral to alkaline pH conditions and that this root response is aggravated by the high Ca concentrations that are common under such conditions. The adverse effect of neutral to alkaline pH conditions on root growth subsequently limits the aboveground growth of rabbiteye blueberry plants.…”

Section: Discussionsupporting

confidence: 71%

“…However, in the current study, no consistent relationship between Ca and P concentrations was observed (Table 4) to support this mechanism. Tamir et al (2019) showed that under neutral pH 7 conditions, in a tissue-culture system, the addition of CaCO 3 to the growth medium decreased the biomass accumulation of rabbiteye blueberry (Vaccinium virgatum Ait., cv. Ochlockonee) plantlets by 35% and increased Ca accumulation, mainly in roots, but also in shoots, without any significant effect on uptake of Fe and Mn per root dry weight unit.…”

Section: Discussionmentioning

confidence: 99%

“…Ochlockonee) plantlets by 35% and increased Ca accumulation, mainly in roots, but also in shoots, without any significant effect on uptake of Fe and Mn per root dry weight unit. It was demonstrated that under conditions of alkaline (pH 8) hydroponic solution, Ca accumulated on the surface and inside the roots (Tamir et al 2019). Zhang et al (2015) reported a negative effect of alkaline pH on conifer trees, which was aggravated by increased concentrations of Ca.…”

Section: Discussionmentioning

confidence: 99%

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Combined Effects of CaCO3 and the Proportion of N-NH4+ Among the Total Applied Inorganic N on the Growth and Mineral Uptake of Rabbiteye Blueberry

Tamir¹,

Zilkah

Dai

et al. 2020

J Soil Sci Plant Nutr

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Blueberry production is limited to acidic soils (pH < 5.5) for reasons that are not thoroughly understood. The objective of this study was to examine the effects of proportion of N-NH 4 + among the total applied inorganic N {N-NH 4 + /(N-NH 4 + +N-NO 3¯) } (RNH 4 +) on rabbiteye blueberry plant growth and mineral uptake under different CaCO 3 soil levels. Rabbiteye blueberry (Vaccinium virgatum Ait., cv. TitanTM) plants were grown in sandy soil mixed with four levels of applied CaCO 3 [0 (control, without any added CaCO 3), 1%, 5%, or 10% (w/w)] in full factorial combination with three levels of RNH 4 + (33%, 66%, or 100%), which were applied through a fertigation system. Leachate pH, biomass accumulation, the concentration of chlorophyll in the leaves, and the concentrations of minerals in leaves and stems were measured. Increased rates of RNH 4 + induced acidification of the leachate solution, which was diminished by the increasing rates of applied CaCO 3 in the soil. Biomass production decreased linearly as the leachate pH rose above 5.5. Biomass production was positively associated with increased Mn concentrations in the leaves and with decreased Ca concentrations in the stems. The current study demonstrates that the application of high levels of RNH 4 + can reduce the pH of neutral and alkaline soils with moderate level of CaCO 3 below the threshold (5.5), and enables the production of rabbiteye blueberry in unsuitable soils.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Combined Effects of CaCO3 and the Proportion of N-NH4+ Among the Total Applied Inorganic N on the Growth and Mineral Uptake of Rabbiteye Blueberry

Tamir¹,

Zilkah

Dai

et al. 2020

J Soil Sci Plant Nutr

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“…These accumulations were associated with the degradation of root morphology (Figure 4). We demonstrated, in a previous study (Tamir et al, 2018), an accumulation of Ca on the root surface of blueberry in alkaline solution too. In that study we presented that this accumulation of Ca decreases gradually from the root surface toward the xylem (Tamir et al, 2018).…”

Section: Discussionsupporting

confidence: 60%

“…Blueberry plants grown in a medium without CaCO 3 produced more biomass than those grown in a similar medium that contained CaCO 3 (Claussen and Lenz, 1999). Similarly, Tamir et al (2018) showed that the biomass production of rabbiteye blueberry (cv. Ochlockonee) plantlets grown in tissue-culture medium that contained 1% (w/w) CaCO 3 was lower than that of plantlets grown in the same medium at the same pH (7.0), but without CaCO 3 .…”

Section: Introductionmentioning

confidence: 83%

Combined effects of alkaline pH and high Ca concentration on root morphology, cell-wall polysaccharide concentrations and blueberry plant performance

Tamir

Zeng²,

Eli³

et al. 2023

Front. Agron.

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The appropriate soil pH for blueberry production is <5.5. However, the exact factors involved in the retardation of blueberry growth at higher pH levels have not yet been completely identified. In this study, we examined the short- and long-term combined effects of pH level and Ca concentrations on root morphology and development in relation to blueberry plant performance. Southern highbush blueberry (Vaccinium corymbosum, cv. Windsor) plants were grown in solutions of pH 4.5 or 7.5 that contained 0, 5, 10, 50 or 100 mg L-1 of Ca. The dimensions of the epidermal cell walls of the roots and the levels of pectin and hemicellulose 1 and 2 in those cells were determined after 1 and 3 days. Concentrations of essential macro and micro-elements in the plant organs, chlorophyll concentrations in the leaves and whole-plant biomass production were measured after 12 weeks. The results demonstrate a rapid (3 days) negative response of root tips at pH 7.5 expressed as reduced dimensions and deformation of the epidermal cell walls. This response was accompanied by reduced concentrations of pectin and hemicellulose 1 in those cell walls. These negative effects of pH 7.5 were amplified by a 100 mg L-1 of Ca over the long term (12 weeks), where the concentrations of P and Ca on the epidermal cell of the root were increased. Additionally, the translocation of Mn from the root to the shoot was retarded. The negative effect of the pH level on roots development and on the Mn translocation were associated with significant reductions in chlorophyll concentrations and biomass production. The results presented in this study demonstrate, for the first time, the negative effect of the combination of slightly alkaline pH and a high Ca concentration on root development, which associated with retarding blueberry plants performance.

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Regeneration of shoots via direct somatic embryogenesis from the leaf surface of Scaevola taccada (Gaertn.) roxb. – a climate resilient species of coastal areas

Shekhawat

Kannan

Manokari

et al. 2021

South African Journal of Botany

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The effects of pH level and calcium carbonate on biomass and mineral uptake of blueberry grown in tissue-culture medium

Cited by 6 publications

References 22 publications

Combined Effects of CaCO3 and the Proportion of N-NH4+ Among the Total Applied Inorganic N on the Growth and Mineral Uptake of Rabbiteye Blueberry

Combined Effects of CaCO3 and the Proportion of N-NH4+ Among the Total Applied Inorganic N on the Growth and Mineral Uptake of Rabbiteye Blueberry

Combined effects of alkaline pH and high Ca concentration on root morphology, cell-wall polysaccharide concentrations and blueberry plant performance

Regeneration of shoots via direct somatic embryogenesis from the leaf surface of Scaevola taccada (Gaertn.) roxb. – a climate resilient species of coastal areas

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