The effects of ozone therapy on periodontal therapy: A randomized placebo‐controlled clinical trial

Abstract

Objectives The aim of this randomized split‐mouth clinical trial was to evaluate the effects of ozone therapy on clinical and biochemical parameters of moderate to severe generalized periodontitis patients after non‐surgical periodontal therapy. Methods A total of 36 moderate to severe generalized periodontitis patients were included in the study. The patients were systemically healthy and 18 to 64 years of age. Periodontal parameters, including plaque index (PI), gingival index (GI), probing depth (PD), percentage of bleeding on probing, percentage of pockets deeper than 5 mm and clinical attachment level (CAL), and percentage of ≥3 mm CAL, were evaluated at baseline and 3 months following periodontal therapy. All participants were treated non‐surgically. Topical gaseous ozone was applied into periodontal pockets twice a week for 2 weeks during active periodontal therapy. Gingival crevicular fluid pentraxin‐3 (PTX‐3), interleukin‐1β (IL‐1β), and high sensitivity C‐reactive protein (Hs‐CRP) were evaluated. All statistical data were analyzed using SPSS software. Results Total of 36 participants completed the study (18 males, 18 females). PI, GI, PD, percentage of bleeding on probing, percentage of pockets deeper than 5 mm and CAL, and percentage of ≥3 mm CAL were improved, and there were no significant differences between the two sides. All inflammatory parameters, PTX‐3, Hs‐CRP, and IL‐1, were reduced at 3‐month follow‐up. Only the decrease in PTX‐3 levels between baseline and 3‐month follow‐up was statistically significant. Conclusions Ozone therapy did not have any additional effect on periodontal parameters. All cytokines were reduced after periodontal therapy. Only PTX‐3 levels were significantly lower at ozone sites compared to those at the control sites.

Ozone Therapy Attenuates NF-κB-Mediated Local Inflammatory Response and Activation of Th17 Cells in Treatment for Psoriasis

“…For instance, ozone therapy ameliorates inflammation and endometrial injury in rats with pelvic inflammatory diseases [34]. Local ozone therapy inhibits the expression of inflammatory factors such as pentraxin-3 (PTX-3), IL-1β, and high-sensitivity C-reactive protein (Hs-CRP) in periodontitis patients [35]. We have shown that local ozone therapy not only can kill Staphylococcus aureus in atopic dermatitis but also can inhibit inflammation by inhibiting the expression of IL-4 [36,37].…”

Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats

“…Although there are several studies indicating that topical ozone application with nonsurgical periodontal treatment has no additional benefit in clinical periodontal parameters, 14 , 30 – 32 few studies have reported significant improvement in periodontal parameters in patients with periodontitis treated with scaling and root planing along with ozone application 15 , 16 . Erdemci, et al 21 (2014) evaluated the effect of SO and TO application after tooth extraction and measured mineralized and trabecular bone and osteoid and osteoblast surfaces for determining alveolar bone healing.…”

Utilização Da Ozonioterapia Em Odontologia/ Use of Ozoniotherapy in Dentistry

“…• Quarenta e um pacientes (20 no grupo experimental, comporto por 14 mulheres e 6 homens) de 23-65 anos; • O ozônio aquoso foi preparado portratando água bi-destilada com gásozônio (75-85 μg/mL) por 10-15 minusando um gerador de ozônio (Hyper-NedezonConforto) resultando numa concentração final de ozônio na água de% 20 μg/mL. Tasdemir Z et al, Turquia (2019) 28 Avaliar os efeitos da terapia com ozônio nos parâmetros clínicos e bioquímicos de pacientes com periodontite generalizada moderada a grave após terapia periodontal não cirúrgica.…”