The Effects of Oxygen on Intestinal Crypt Survival in Cobalt-60-Irradiated Mice

Sigdestad, Curtis P.; Hagemann, Ronald F.; Scott, Ralph M.

doi:10.2307/3573868

Cited by 10 publications

(1 citation statement)

References 3 publications

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“…Hypoxia has been shown to increase intestinal stem cell survival [78,90], and a shift in the cell age distribution of clonogenic cells toward mid to late S-phase of the cell cycle can result in an increased survival after photon irradiation [44,47], The beneficial action of radiosensitizers is believed to be due to their oxidizing activity, which pro motes the formation of high energy radicals leading to the subsequent tissue damage. Consequently, they are only effective in anoxic tissue and their presence in well-oxy genated tissue is superfluous [96,104], Many tumors are anoxic because of their very high metabolic rate, and so the action of these agents is apparently ideal; only tumors will be sensitized to the radiation dose, while normal well-oxygenated tissue will be unaf fected.…”

Section: Methods For Preventing Radiation Enteropathymentioning

confidence: 99%

Radiation and the Small Intestine

Churnratanakul

Wirzba

Lam

et al. 1990

Dig Dis

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Section: Methods For Preventing Radiation Enteropathymentioning

confidence: 99%

Radiation and the Small Intestine

Churnratanakul

Wirzba

Lam

et al. 1990

Dig Dis

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Misonidazole-induced Radio-resistance in Normal and Pre-irradiated Jejunal Muscosa

Daly

Julia

Malaise

1988

International Journal of Radiation Biology

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Pretreatment of mice with a single dose of whole-abdomen irradiation led to a relative decrease in radiosensitivity in jejunal crypts given a second single dose of irradiation 2 months later. Hyperbaric oxygen (3.5 bars) restored the survival level to initial values, suggesting that there was radiation-induced hypoxia in the primed jejunum. However, misonidazole did not sensitize primed jejunal crypts; it reduced the radiosensitivity of both normal and primed crypts. This 'paradoxical' effect of misonidazole could well be due to the acute toxic side-effects of 1 mg/g body weight misonidazole i.p., as there was a sharp drop in the core temperature of mice immediately after drug injection. Artificially maintaining the temperature of miso-treated animals at a normal level produced crypt survival levels similar to those of both normal controls and primed controls. Thus, although the primed gut is chronically hypoxic, as suggested by the effects of hyperbaric oxygen, misonidazole is not a reliable tool for the study of this tissue hypoxia. In all in vivo experiments with misonidazole, core temperature must be controlled in order to avoid misleading interpretations of experimental results.

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Care with radiosensitizers

Hendry¹,

Sutton

1978

BJR

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The Effects of Oxygen on Intestinal Crypt Survival in Cobalt-60-Irradiated Mice

Cited by 10 publications

References 3 publications

Radiation and the Small Intestine

Radiation and the Small Intestine

Misonidazole-induced Radio-resistance in Normal and Pre-irradiated Jejunal Muscosa

Care with radiosensitizers

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