The Effects of Oral Consumption of Selenium Nanoparticles on Chemotactic and Respiratory Burst Activities of Neutrophils in Comparison with Sodium Selenite in Sheep

Abstract

The present study was designed to compare the effects of nano-selenium and of sodium selenite on the chemotactic and respiratory burst activities of neutrophils in sheep. Fifteen sheep were randomly divided into three groups. Groups 1 and 2 received selenium nanoparticles (1 mg/kg) or sodium selenite (1 mg/kg) orally, respectively, for ten consecutive days, and the third group was considered as the control. To determine the chemotactic and respiratory burst activities of the neutrophils, the leading front assay and the NBT test were used on heparinized blood samples that were collected at different intervals (days 0, 10th, 20th, and 30th). The results obtained showed that the chemotactic activities in groups 1 and 2 increased significantly on the 10th, 20th, and 30th day, compared to day 0, and on the 20th day in comparison with the 10th day, while in group 2, there was a significant decrease on the 30th day compared to the 20th day. The chemotactic activities in group 1 were significantly higher than in group 2 on the 10th day and in the control group on the 10th, 20th, and 30th day, but the chemotactic activities in group 2 were significantly higher than those in the control group only on the 20th day. On the 30th day into the experiment, the respiratory bursts in groups 1 and 2 were significantly stronger in comparison with those at day 0. Overall, nano-selenium increased the chemotactic and respiratory burst activities more significantly than sodium selenite, which is suggestive of a stronger stimulatory effect of the Se nanoparticles on intracellular activities.

Subacute oral toxicity investigation of selenium nanoparticles and selenite in rats

“…We note that results from our study and from published results showed that the same toxicity endpoints are affected by both SeNPs and dissolved ionic Se species (Zhang et al 2001, Jia et al 2005, Wang et al 2007, Benko et al 2012, Kojouri, Sadeghian, et al 2012, Hadrup et al 2016). An explanation for this could be that SeNP are dissolved in the mammalian body to exert an effect similar to that observed for dissolved selenite.…”

“…Moreover, in a previous study based on the same animal experiment we found that both Se formulations displayed similar effects on the general urine metabolome as indicated by partial least squares discriminant analyses, with specific differences in such putative identified metabolites as decanedioic acid and hydroxydecanedioic acid (Hadrup et al 2016). Data from other studies show that ionic forms of Se exert toxicity at lower doses as compared to SeNPs (Zhang et al 2001, Jia et al 2005, Wang et al 2007, or that equal or almost equal effects occur following dosage of the two formulations (Benko et al 2012, Kojouri, Jahanabadi, et al 2012, Kojouri, Sadeghian, et al 2012. Combined with results from our study, the available data did not lead to any firm conclusion on whether SeNPs are less or more toxic as compared to dissolved Na selenite, i.e.…”

“…In a 13-week rat feeding study, only a slight difference in toxicity between the same two Se preparations was observed based on haematology and organ weight; administered doses were 0.1, 0.2, 0.3 and 0.4 mg Se/kg bw/day as SeNP (20-60 nm in diameter) or selenite and the no observed adverse effect level (NOAEL) was 0.1 mg/kg bw for selenite as compared to NOAEL of 0.2 mg/kg bw for SeNP (Jia et al 2005). Similar toxicity of SeNP and selenite was demonstrated in sheep; an oral dose of 1 mg/kg Se bw/day of SeNPs (smaller than 220 nm) or selenite administered for 10 days resulted in similar effects on serum iron, transferrin expression and increased neutrophil chemotactic activities (Kojouri, Jahanabadi, et al 2012, Kojouri, Sadeghian, et al 2012.…”

Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—as determined by metabolite pattern determination

“…Data from others are also in line with this. Similar effects of Se nanoparticles and sodium selenite on iron, transferrin and on neutrophils have been observed in sheep ( Kojouri et al, 2012a ; Kojouri et al, 2012b ). In contrast, a higher biological effect of Se as selenite ions as compared to nanoparticles has been reported in other studies.…”

Reducción de selenito a Selenio elemental por Pantoea agglomerans

“…Reportes indican que esta actividad biológica es comparable a la que muestran el selenito, el seleniato o bien algunas formas orgánicas de selenio (selenometionina, selenocisteína, etc.) (Kojouri et al 2012 por lo que son una alternativa para reemplazar a cualquiera de estas especies actualmente utilizadas como suplementos de selenio en distintos países.…”