The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer’s disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line

Mairuae, Nootchanat; Connor, James R.; Lee, Sang Yong; Cheepsunthorn, Poonlarp; Tongjaroenbuangam, Walaiporn

doi:10.1016/j.neulet.2015.07.011

Cited by 17 publications

(12 citation statements)

References 24 publications

(32 reference statements)

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“…The form of iron used to treat the cells in the present study was ferric citrate ammonium (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Levels of intracellular iron were measured using calcein acetoxymethyl ester (calcein-AM) (21,22). This reagent contains AM ester derivatives of fluorescent indicators, and its lipophilic structure is able to permeate cell membranes.…”

Section: Methodsmentioning

confidence: 99%

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

Mairuae

Cheepsunthorn

2018

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Abstract. Iron accumulation in activated microglia has been consistently reported in neurodegenerative diseases. Previous results suggest that these cells facilitate neuroinflammation leading to neuronal cell death. Therefore, chemical compounds that alleviate the activation of iron-rich microglia may result in neuroprotection. In the present study, the effect of valproic acid (VPA) on microglial activation under iron-rich conditions was investigated. BV-2 microglial cells were exposed to lipopolysaccharide (LPS; 1 µg/ml) and iron (300 µg/ml) with or without VPA (1.6 mM). The results demonstrated that VPA attenuated the activation of iron-rich BV2 cells induced by LPS by down-regulating the mRNA expression of inducible nitric oxide (NO) synthase and interleukin 1β (IL-1β; P<0.01), to ultimately reduce the production of NO and IL-1β (P<0.01). These events were accompanied by an attenuation in the nuclear translocation of nuclear factor-κB p65 subunit (P<0.01). These findings suggest that VPA may be therapeutically useful for attenuating the activation of iron-rich microglia.

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Section: Methodsmentioning

confidence: 99%

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

Mairuae

Cheepsunthorn

2018

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“…Yu et al [53] demonstrated the neuroprotective role of rutin against amylin-induced neurocytotoxicity in neuronal cells and concluded that the antioxidant property of rutin might have played a role in the protection of neuronal cells. Mairuae et al [54] showed the in vitro neuroprotective effect of okra in SH-SY5Y cells and suggested that the antioxidant effect of okra was responsible for the protective role. Uddin et al [55] pointed out that the potent phenolic antioxidants present in the Vanda roxburghii could be responsible for the inhibition of the activation of acetylcholinesterase and butyrylcholinesterase.…”

Section: Natural Products and Alzheimer's Diseasementioning

confidence: 99%

The Role of Reactive Oxygen Species in the Pathogenesis of Alzheimer’s Disease, Parkinson’s Disease, and Huntington’s Disease: A Mini Review

Manoharan

Guillemin

Abiramasundari

et al. 2016

Oxidative Medicine and Cellular Longevity

396

289

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Neurodegenerative diseases affect not only the life quality of aging populations, but also their life spans. All forms of neurodegenerative diseases have a massive impact on the elderly. The major threat of these brain diseases includes progressive loss of memory, Alzheimer's disease (AD), impairments in the movement, Parkinson's disease (PD), and the inability to walk, talk, and think, Huntington's disease (HD). Oxidative stress and mitochondrial dysfunction are highlighted as a central feature of brain degenerative diseases. Oxidative stress, a condition that occurs due to imbalance in oxidant and antioxidant status, has been known to play a vital role in the pathophysiology of neurodegenerative diseases including AD, PD, and HD. A large number of studies have utilized oxidative stress biomarkers to investigate the severity of these neurodegenerative diseases and medications are available, but these only treat the symptoms. In traditional medicine, a large number of medicinal plants have been used to treat the symptoms of these neurodegenerative diseases. Extensive studies scientifically validated the beneficial effect of natural products against neurodegenerative diseases using suitable animal models. This short review focuses the role of oxidative stress in the pathogenesis of AD, PD, and HD and the protective efficacy of natural products against these diseases.

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“…Okra extract and its bioactive constituents, quercetin and rutin, also protect neuronal function and improve learning and memory deficits in dexamethasone-treated mice [41]. Recently, a study demonstrated that okra treatment decreases ROS and H 2 O 2 production, tau phosphorylation, and cellular events associated with AD in a stably expressed HFE neuroblastoma SH-SY5Y cell line [42]. Literature review revealed that okra attenuates the production of the proin-…”

Section: Neurological Disordersmentioning

confidence: 99%

“…The lady's finger provides important in vitro data on the effects of various AlD-associated cellular processes in H63D variant HFE cells. These results suggest it may be beneficial in people expressing the H63D variant to reduce the risk of Alzheimer's disease and other neurodegenerative diseases related to oxidative stress[44] [45].…”

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confidence: 99%

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant

Chowdhury¹,

Jamaly²,

Farjana³

et al. 2019

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Abelmoschus is a genus of about fifteen species belongs to the family Malvaceae. The herb, popularly known as Lady's Finger or Okra (in English) is a nutritional source used for both medicinal and culinary purposes. The plant is widely distributed from Africa to Asia, Southern Europe, and America. This comprehensive account provides a botanical description of the plant, its phytochemical constituents and pharmacological activities focusing anti-diabetic, anti-oxidant, anti-adhesive, gastro-protective, hepatoprotective, and immunomodulating actions. Most of the pharmacological effects can be explained by the constituents like tannins, terpenoids, flavonoids and glycosides present in all plant parts. However, future efforts should concentrate more on in vitro and in vivo studies and also on clinical trials in order to confirm traditional wisdom in the light of a rational phytotherapy. The present review is an overview of phytochemistry and ethnopharmacological studies that support many of the traditional ethnomedicinal uses of the plant.

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The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer’s disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line

Cited by 17 publications

References 24 publications

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

The Role of Reactive Oxygen Species in the Pathogenesis of Alzheimer’s Disease, Parkinson’s Disease, and Huntington’s Disease: A Mini Review

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant

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The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer’s disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line

Cited by 17 publications

References 24 publications

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

The Role of Reactive Oxygen Species in the Pathogenesis of Alzheimer’s Disease, Parkinson’s Disease, and Huntington’s Disease: A Mini Review

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (&lt;i&gt;Abelmoschus esculentus&lt;/i&gt; L.) Plant

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A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant