The effects of ochratoxin A on lipid peroxidation and antioxidant enzymes: a protective role of melatonin

Soyöz, Mustafa; Özçelik, Nurten; Kılınç, İbrahim; Altuntaş, İrfan

doi:10.1023/b:cbto.0000038459.98032.34

Cited by 27 publications

(25 citation statements)

References 25 publications

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“…In our study, rats treated with OTA+MEL had reduced levels of MDA and increased GPx activities in liver and kidney compared with OTA-treated rats (Baundrimont et al 1994;Soyoz et al 2004;Oz and Ilhan 2006). It is most likely that the ability of MEL to stimulate the synthesis of antioxidants enzyme may lead to reduction of MDA levels and increase the GPx.…”

Section: Discussionsupporting

confidence: 50%

Protective Role of Melatonin and Coenzyme Q10 in Ochratoxin A Toxicity in Rat Liver and Kidney

et al. 2007

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Melatonin (MEL) and coenzyme Q10 (CoQ10) both display antioxidant and free radical scavenger properties. In the present study, the effect of MEL and CoQ10 on the oxidative stress and fibrosis induced by ochratoxin A (OTA) administration in rats was investigated. Rats were divided into five equal groups, each consisting of seven rats: (1) controls; (2) OTA-treated rats (289 microg/kg/day); (3) OTA+MEL-treated rats (289 microg/kg/day OTA + 10 mg/kg/day MEL); and (4) OTA+CoQ10-treated rats (289 microg/kg/day OTA + 1 mg/100 g/day body weight (bw) CoQ10). After 4 weeks of treatment, the level of malondialdehyde (MDA), glutathione peroxidase (GPx), and hydroxyproline (Hyp) were measured in the homogenates of liver and kidney. In the OTA-treated group, the levels of MDA and Hyp in both liver and kidney were significantly increased when compared with the levels of control, whereas GPx activities decreased. In OTA+MEL-treated rats, the levels of MDA and Hyp in both liver and kidney were significantly decreased when compared with the levels of OTA-treated rats; however; GPX activities increased. In the OTA+CoQ10-treated group, the levels of MDA and Hyp were decreased when compared with the levels of OTA-treated rats, whereas GPx activities increased. In the OTA+CoQ10-treated group, the levels of MDA, Hyp, and GPx were not significantly changed in kidney when compared with OTA-treated group. MEL has a protective effect against OTA toxicity through an inhibition of the oxidative damage and fibrosis both liver and kidney. Although CoQ10 has protective effect against OTA toxicity in liver tissue, it has no effect in kidney tissue.

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Section: Discussionsupporting

confidence: 50%

Protective Role of Melatonin and Coenzyme Q10 in Ochratoxin A Toxicity in Rat Liver and Kidney

et al. 2007

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“…Since GSH acts as the substrate for GPx, the decreased availability of GSH for the activity of GPx would result in the accumulation of H 2 O 2 . LA treatment restored catalase activity in irradiated fibroblasts; which may be attributed to its free radical scavenging property and its ability to increase cellular levels of NADPH and NADH (Soyöz et al 2004); catalase requires NADPH for its regeneration from inactive form.…”

Section: Radiation Dosagementioning

confidence: 92%

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

et al. 2008

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During the course of cancer radiation treatment, normal skin invariably suffers from the cytotoxic effects of gamma-radiation and reactive oxygen species (ROS), which are generated from the interaction between radiation and the water molecules in cells. The present study was designed to investigate the radioprotective role of alpha-lipoic acid (LA), an antioxidant on murine skin fibroblasts exposed to a single dose of 2, 4, 6, or 8Gy gamma-radiation. Irradiation of fibroblasts significantly increased ROS, nitric oxide, and lipid peroxidation (P < 0.001); all of these factors substantially decreased with 100 microM LA treatment. Hydroxyl radical (OH(.)) production from 8Gy irradiated fibroblasts was measured directly by electron spin resonance using spin-trapping techniques. LA was found to inhibit OH(.) production at 100-microM concentrations. Dose-dependent depletion of antioxidants, such as catalase and glutathione reductase, was observed in irradiated fibroblasts (P < 0.001), along with increased superoxide dismutase (P < 0.001). LA treatment restored antioxidant levels. Concentration of the pro-inflammatory cytokine IL-1beta was significantly reduced in irradiated fibroblasts when treated with LA. MTT and lactate dehydrogenase assays demonstrated that LA treatment reduced cell injury and protected cells against irradiation-induced cytotoxicity. Thus, we conclude that results are encouraging and need further experiments to demonstrate a possible benefit in cancer patients and the reduction of harmful effects of radiation therapy.

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“…A strong antioxidant effect of melatonin seems evident as almost all investigators describe that in liver homogenates, melatonin strongly attenuated hepatic LPO [40][41][42][43][44][45][46][47][48][49]102,103,[106][107][108][109][110][111][112][113][114][116][117][118][119][120][121][122][123] , usually measured by means of malondialdehyde quantification. Furthermore, melatonin appears to increase the activity and/or expression of hepatic antioxidant enzymes, such as GSH, GPx and SOD, after most types of injury [40][41][42][43][44][45][46][47][48][49][53][54][55][56][57][58][59][60][61][62][63][97][98][99]…”

Section: Antioxidant Effectsmentioning

confidence: 99%

“…Furthermore, melatonin appears to increase the activity and/or expression of hepatic antioxidant enzymes, such as GSH, GPx and SOD, after most types of injury [40][41][42][43][44][45][46][47][48][49][53][54][55][56][57][58][59][60][61][62][63][97][98][99]103,[106][107][108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][125][126][127][130][131][132][133][134][135][136][137]…”

Section: Antioxidant Effectsmentioning

confidence: 99%

Hepatoprotective actions of melatonin: Possible mediation by melatonin receptors

Mathes¹

2010

WJG

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Melatonin, the hormone of darkness and messenger of the photoperiod, is also well known to exhibit strong direct and indirect antioxidant properties. Melatonin has previously been demonstrated to be a powerful organ protective substance in numerous models of injury; these beneficial effects have been attributed to the hormone's intense radical scavenging capacity. The present report reviews the hepatoprotective potential of the pineal hormone in various models of oxidative stress in vivo, and summarizes the extensive literature showing that melatonin may be a suitable experimental substance to reduce liver damage after sepsis, hemorrhagic shock, ischemia/reperfusion, and in numerous models of toxic liver injury. Melatonin's influence on hepatic antioxidant enzymes and other potentially relevant pathways, such as nitric oxide signaling, hepatic cytokine and heat shock protein expression, are evaluated. Based on recent literature demonstrating the functional relevance of melatonin receptor activation for hepatic organ protection, this article finally suggests that melatonin receptors could mediate the hepatoprotective actions of melatonin therapy.

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The effects of ochratoxin A on lipid peroxidation and antioxidant enzymes: a protective role of melatonin

Cited by 27 publications

References 25 publications

Protective Role of Melatonin and Coenzyme Q10 in Ochratoxin A Toxicity in Rat Liver and Kidney

Protective Role of Melatonin and Coenzyme Q10 in Ochratoxin A Toxicity in Rat Liver and Kidney

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

Hepatoprotective actions of melatonin: Possible mediation by melatonin receptors

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