The effects of oat bran and soy flour on humoral immune response in rats fed hypercholesterolaemic diets

Abstract

Estudos anteriores têm demonstrado os efeitos sobre o sistema imune e sobre os níveis de colesterol da dieta com fibra solúvel e proteína de soja em ratos. Nesse estudo, nós avaliamos os efeitos do farelo de aveia e da farinha de soja na resposta imune humoral de ratos Wistar tratados com dietas hipercolesterolêmicas. Os animais (6 grupos) foram alimentados com diferentes dietas por 6 semanas e inoculados duas vezes com antígeno (IgY). As amostras de plasma foram coletadas após cada inoculação e os níveis de anticorpos anti-IgY (IgM, IgG1 e IgG2a) foram avaliados por ELISA. Os animais que receberam 1% de colesterol apresentaram um aumento de IgG1 anti-IgY e uma redução de IgM e IgG2a anti-IgY em relação aos animais controle. Estes efeitos foram anulados em animais alimentados com ARTIGOS / ARTICLES CIÊNCIAS BIOLÓGICAS E DA SAÚDEPrevious studies have demonstrated the immunological and hypercholesterolaemic effects of soluble dietary fibre and soy protein in rats. In this study, we evaluated the effects of oat bran and soy flour on humoral immune response in Wistar rats fed hypercholesterolaemic diets. Animals (6 groups) fed with different diets for 6 wks were inoculated twice with antigen (IgY). Plasma samples were collected after each inoculation and anti-IgY antibody (IgM, IgG1 and IgG2a) levels were evaluated by ELISA. Animals receiving 1% cholesterol presented an increase in anti-IgY IgG1 and a reduction in anti-IgY IgM and IgG2a relative to control animals. These effects were abrogated in animals fed 1% cholesterol and oat bran or oat bran+soy protein, but not in animals fed 1% cholesterol and soy protein. Diets containing 1% cholesterol resulted in hepatic lesions and higher liver and spleen relative weights, but did not affect lipid profile, weight gain, food intake, or food conversion efficiency. In conclusion, a high-cholesterol diet influences classes of antibodies produced in response to an antigen in a way that can be reversed by oat bran. Resumo

Effect of dietary plants on the production of immunoglobulins A in healthy Wistar rats

“…The use of plants and their parts as therapeutic agents is a common practice. (1)(2) Many have medicinal properties and their application, especially in traditional medicine, is well recognized and established. Although they have some aspects similar to modern drugs, in general, they are less aggressive and safer.…”